A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus

Wu, Yanping; Bai, Jinrong; Zhong, Kai; Huang, Yina; Gao, Hong

doi:10.1016/j.foodchem.2016.07.090

Cited by 132 publications

(59 citation statements)

References 36 publications

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“…DMY not only changed membrane integrity, fluidity and membrane protein conformation, but also bound to intracellular DNA through the groove-binding mode in S. aureus. DMY achieved bactericidal activity by dual effects of cell membrane damage and DNA binding (Wu, Bai, Zhong, Huang, & Gao, 2017). However, in the study of Huang, Huang, Chen, Yang, and Huang (2015), the effect of DMY on the inhibition of S. aureus PriA (SaPriA), an essential helicase for DNA replication restart, was not so significant.…”

Section: Antimicrobial and Antiviral Activitymentioning

confidence: 98%

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Liú

Mao

Ding

et al. 2019

Trends in Food Science & Technology

114

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Background: Dihydromyricetin (DMY) is an important plant flavonoid, which has received great attention due to its health-benefiting activities, including antioxidant, antimicrobial, anti-inflammatory, anticancer, antidiabetic and neuroprotective activities. DMY capsules have been sold in US as a nutraceutical supplement to prevent alcoholic hangovers. The major disadvantage associated with DMY is its chemical instability and poor bioavailability caused by the combined effects of its low solubility and poor membrane permeability. This limits its practical use in the food and pharmaceutical fields. Scope and approach: The present paper gives an overview of the current methods for the identification and quantification of DMY. Furthermore, recent findings regarding the main biological properties and chemical stability of DMY, the metabolism of DMY as well as different approaches to increase DMY bioavailability in both aqueous and lipid phases are discussed. Key findings and conclusions: Current trends on identification and quantification of DMY have been focused on spectral and chromatographic techniques. Many factors such as heat, pH, metal ions, could affect the chemical stability of DMY. Despite the diverse biological effects of DMY, DMY faces with the problem of poor bioavailability. Utilization of different delivery systems including solid dispersion, nanocapsule, microemuslion, cyclodextrin inclusion complexes, co-crystallization, phospholipid complexes, and chemical or enzymatic acylation has the potential to improve both the solubility and bioavailability. DMY digested in laboratory animals undergoes reduction, dehydroxylation, methylation, glucuronidation, and sulfation. Novel DMY delivery systems and basic pharmacokinetic studies of encapsulated DMY on higher animals and humans might be required in the future.

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Section: Antimicrobial and Antiviral Activitymentioning

confidence: 98%

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Liú

Mao

Ding

et al. 2019

Trends in Food Science & Technology

114

View full text Add to dashboard Cite

show abstract

“…Dihydromyricetin (DMY), the most abundant and bioactive flavonoid in vine tea, could reach the content of more than 30%, which is rarely seen and unique in plant kingdom (Wang, Xiong, Reddy Perumalla, Fang, & Sun Calvin 2016). The antioxidant (Liao et al, 2014), anti-inflammation (Hou et al, 2015b;Liu et al, 2017), and antibacterial (Wu, Bai, Zhong, Huang, & Gao, 2017) effects of DMY were already demonstrated in vitro. The anti-alcoholic intoxication of DMY on the animal models was also reported (Shen et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Interactions of Dihydromyricetin, a Flavonoid from Vine Tea (Ampelopsis grossedentata) with Gut Microbiota

Fan

Zhao

Tong

et al. 2018

Journal of Food Science

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“…Dihydromyricetin (DMY), a type of flavonoid, is the main bioactive component of Ampelopsis grossedentata, which is a widely distributed herbal medicine in Southern China (Okazaki et al, 2005). Previous reports demonstrated that DMY has numerous biologic and pharmacological activities, including antioxidative, anti-inflammatory, antiapoptotic, anticarcinogenic, and antidiabetic effects Li et al, 2015;Ye et al, 2015;Le et al, 2016;Wu et al, 2017). Considering that DMY possesses an anti-inflammatory property, we investigated its potential capacity to inhibit the chronic autoimmune inflammation of RA.…”

Section: Introductionmentioning

confidence: 99%

Dihydromyricetin Inhibits Inflammation of Fibroblast-Like Synoviocytes through Regulation of Nuclear Factor-κB Signaling in Rats with Collagen-Induced Arthritis

Zhao

Fan

et al. 2018

J Pharmacol Exp Ther

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Dihydromyricetin (DMY), the main flavonoid of Ampelopsis grossedentata, has potent anti-inflammatory activity. However, the effect of DMY on chronic autoimmune arthritis remains undefined. In this study, we investigated the therapeutic effects of DMY on collageninduced arthritis (CIA). Wistar rats were immunized with bovine type II collagen to establish CIA and were then administered DMY intraperitoneally (5, 25, and 50 mg/kg) every other day for 5 weeks. Paw swelling, clinical scoring, and histologic analysis were assessed to determine the therapeutic effects of DMY on the development of arthritis in CIA rats. The results showed that treatment with DMY significantly reduced erythema and swelling in the paws of CIA rats. Pathologic analysis of the knee joints and peripheral blood cytokine assay results confirmed the antiarthritic effects of DMY on synovitis and inflammation.Fibroblast-like synoviocytes (FLSs) were isolated from the synovium of CIA rats and treated with 10 ng/ml interleukin (IL)-1b. DMY significantly inhibited the proliferation, migration, and inflammation of IL-1b-induced FLSs, whereas it significantly increased IL-1b-induced FLS apoptosis in a dosedependent manner (6.25-25 mM). Moreover, DMY suppressed phosphorylation of IkB kinase (IKK) and inhibitor of NF-kB a and subsequently reduced the IL-1b-induced nucleus translocation of NF-kB in FLSs. Through a molecular docking assay, we demonstrated that DMY could directly bind to the Thr9 and Asp88 residues in IKKa and the Asp95, Asn142, and Gln167 residues in IKKb. These findings demonstrate that DMY could alleviate inflammation in CIA rats and attenuate IL-1b-induced activities in FLSs through suppression of NF-kB signaling.

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A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus

Cited by 132 publications

References 36 publications

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Interactions of Dihydromyricetin, a Flavonoid from Vine Tea (Ampelopsis grossedentata) with Gut Microbiota

Dihydromyricetin Inhibits Inflammation of Fibroblast-Like Synoviocytes through Regulation of Nuclear Factor-κB Signaling in Rats with Collagen-Induced Arthritis

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