A dual extraction technique combined with HPLC-ICP-MS for speciation of seleno-amino acids in rice and yeast samples

Guo, Xueqin; He, Man; Nan, Kai; He, Yan; Chen, Beibei; Hu, Bin

doi:10.1039/c5ja00286a

Cited by 16 publications

(5 citation statements)

References 26 publications

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“…39,40 Once inside the cells, Se (+4) ions are reduced to Se (−2) ions, then the Cd 2+ interacts with the seleno-precursors and forms QDs crystals. The main seleno-amino acids emerging during the biosynthesis, as the previous study suggested, 26 were detected by HPLC-ICP-MS. 41 In Figure 5, it is shown that the major seleno-amino acid peak heights in Δatp1 and WT samples were consistent with the fluorescence intensities between them ( Figure 2B). The result was proof that the accumulation of the seleno-precursor was an energy-consuming process.…”

Section: Discussionmentioning

confidence: 50%

ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in <em>Saccharomyces cerevisiae</em>

Zhang¹,

Shao

Han

et al. 2017

IJN

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Due to a growing trend in their biomedical application, biosynthesized nanomaterials are of great interest to researchers nowadays with their biocompatible, low-energy consumption, economic, and tunable characteristics. It is important to understand the mechanism of biosynthesis in order to achieve more efficient applications. Since there are only rare studies on the influences of cellular energy levels on biosynthesis, the influence of energy is often overlooked. Through determination of the intracellular ATP concentrations during the biosynthesis process, significant changes were observed. In addition, ATP synthesis deficiency caused great decreases in quantum dots (QDs) biosynthesis in the Δ atp1 , Δ atp2 , Δ atp14 , and Δ atp17 strains. With inductively coupled plasma-atomic emission spectrometry and atomic absorption spectroscopy analyses, it was found that ATP affected the accumulation of the seleno-precursor and helped with the uptake of Cd and the formation of QDs. We successfully enhanced the fluorescence intensity 1.5 or 2 times through genetic modification to increase ATP or SeAM (the seleno analog of S -adenosylmethionine, the product that would accumulate when ATP is accrued). This work explains the mechanism for the correlation of the cellular energy level and QDs biosynthesis in living cells, demonstrates control of the biosynthesis using this mechanism, and thus provides a new manipulation strategy for the biosynthesis of other nanomaterials to widen their applications.

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Section: Discussionmentioning

confidence: 50%

ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in <em>Saccharomyces cerevisiae</em>

Zhang¹,

Shao

Han

et al. 2017

IJN

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“…In order to avoid the alteration of the sample during the preconcentration process, a solution of ethylenediamine was used as eluent. This method showed the following analytical performance for the analysis of Se compounds (SeCys 2 , MeSeCys, SeMet, and SeEt) in rice: linear range 158e278 mg L À1 , LOD 0.0075e0.013 mg L À1 and standard deviation 6.8e10.2% [90].…”

Section: Seleniummentioning

confidence: 94%

“…Selenium is an essential element with antioxidant and antitumoral effects [89], but the excess of this element can produce serious damage in the organism [90]. The toxicity of Se depends on the concentration and the chemical form, being more toxic selenite and selenate than the organic compounds such as selenoaminoacids, selenopeptides, selenoproteins, etc.…”

Section: Seleniummentioning

confidence: 99%

“…Guo et al reported a MSPE combined with a hole fibre liquid-liquid microextraction (HF-LLLME), using MGO (Fe 3 O 4 @GO) modified with polyaniline (PANI) and Cu II as sorbent (Fe 3 O 4 @SiO 2 @PANI@GO/Cu) and ICP MS as detector. Coordinated complexes of polar selenoaminoacids and Cu II were formed, allowing the speciation [90]. In order to avoid the alteration of the sample during the preconcentration process, a solution of ethylenediamine was used as eluent.…”

Section: Seleniummentioning

confidence: 99%

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Magnetic graphene oxide as a valuable material for the speciation of trace elements

Morales-Benítez

Montoro-Leal²,

García-Mesa³

et al. 2022

TrAC Trends in Analytical Chemistry

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“…The second concern was the operation of rGO. Because the high-speed centrifugation-based collection of rGO dispersed in water is time-consuming, , GO or rGO has been combined with magnetic beads as a magnetic solid-phase extraction (MSPE) material in many studies for the extraction of a variety of molecules such as amino acids and proteins, making the collection of GO/rGO easier and faster. However, to the best of our knowledge, GO or rGO-based MSPE materials have never been used to extract miRNA before.…”

mentioning

confidence: 99%

Reduced Graphene Oxide-Based Solid-Phase Extraction for the Enrichment and Detection of microRNA

Lü

et al. 2017

Anal. Chem.

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MicroRNAs (miRNAs) are endogenous molecules with regulatory functions. The purification and enrichment of miRNA are essential for its precise and sensitive detection. miRNA isolated using commercial kits contains abundant interfering RNAs, and the concentration of miRNA may not be adequate for detection. Herein, we prepared a reduced graphene oxide (rGO)-based magnetic solid-phase extraction material for the enrichment and ultrasensitive detection of miRNA from intricate nucleic acid solutions. In situ reverse transcription (RT) was developed as the most efficient approach to desorb miRNA from rGO among the methods that are compatible for the subsequent amplification reported thus far. Additionally, rolling circle amplification and qPCR were used to detect let-7a with a decrease of the limit of detection by 24.7- and 31.3-fold, respectively. This material was also successfully used to extract and detect miRNA from total RNA isolated from human plasma. Our results show that the material prepared in this study has the potential for cancer biopsy in clinics and the discovery of new miRNAs in scientific research.

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A dual extraction technique combined with HPLC-ICP-MS for speciation of seleno-amino acids in rice and yeast samples

Abstract: MSPE combined with HF-LLLME for speciation of seleno-amino acids with high EFs and good anti-interference ability.

Cited by 16 publications

References 26 publications

ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in <em>Saccharomyces cerevisiae</em>

ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in <em>Saccharomyces cerevisiae</em>

Magnetic graphene oxide as a valuable material for the speciation of trace elements

Reduced Graphene Oxide-Based Solid-Phase Extraction for the Enrichment and Detection of microRNA

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