A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells

Wang, Zeming; Detomasi, Tyler C.; Chang, Christopher J.

doi:10.1039/d0sc03844j

Cited by 30 publications

(24 citation statements)

References 70 publications

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“…Extracellular K + regulates cellular osmolality, cellular environmental homeostasis, and membrane potential. An abnormally fluctuant concentration of potassium ion above the maximal background concentration (extracellular 5 mM K + ) can cause neurological disorder, diabetes, kidney failure, heart disorder, and various cancers ( 36 , 37 ). The K + -specific signal convertor was based on G-quadruplex–mediated transduction, in which a fluctuating concentration of K + could be converted into a fluctuating concentration of strand I released from complex AI (fig.…”

Section: Resultsmentioning

confidence: 99%

“…S13). Then, we defined above 5 mM K + as the meaningful stimulus of our system and set the cutoff value of the threshold gate (BC) to see whether K + -specific DMPS could filter out background K + noise and accumulate dynamic signal only when environmental K + exceeded 5 mM ( 37 ). When AI:BC was 4:1, there was an obvious fluorescence rise from 5 to 6 mM K + (fig.…”

Section: Resultsmentioning

confidence: 99%

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Network topology–directed design of molecular CPU for cell-like dynamic information processing

et al. 2022

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Natural cells (NCs) can automatically and continuously respond to fluctuant external information and distinguish meaningful stimuli from weak noise depending on their powerful genetic and protein networks. We herein report a network topology–directed design of dynamic molecular processing system (DMPS) as a molecular central processing unit that powers an artificial cell (AC) able to process fluctuant information in its immediate environment similar to NCs. By constructing a mixed cell community, ACs and NCs have synchronous response to fluctuant extracellular stimuli under physiological condition and in a blood vessel–mimic circulation system. We also show that fluctuant bioinformation released by NCs can be received and processed by ACs. The molecular design of DMPS-powered AC is expected to allow a profound understanding of biological systems, advance the construction of intelligent molecular systems, and promote more elegant bioengineering applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Network topology–directed design of molecular CPU for cell-like dynamic information processing

et al. 2022

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“…Improved APG-2 ( Figure 5B ) and APG-4 were applied in permeabilized Jurkat and U937 cells to visualize changes in intracellular K + concentration ( Rana et al, 2019 ). Recently, Ratiometric Potassium Sensor-1 (RPS-1) was developed with dual fluorophores: a K + -responsive fluorophore Potassium Sensor 525 (PS525) and a non-responsive Coumarin 343 for internal calibration in living tissues ( Wang et al, 2021 ). The dye displays a ∆ F / F 0 of 6 and an apparent K d of 137 mM.…”

Section: K + Indicatorsmentioning

confidence: 99%

Fluorescent Indicators For Biological Imaging of Monatomic Ions

Shen

Shkolnikov

et al. 2022

Front. Cell Dev. Biol.

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Monatomic ions play critical biological roles including maintaining the cellular osmotic pressure, transmitting signals, and catalyzing redox reactions as cofactors in enzymes. The ability to visualize monatomic ion concentration, and dynamic changes in the concentration, is essential to understanding their many biological functions. A growing number of genetically encodable and synthetic indicators enable the visualization and detection of monatomic ions in biological systems. With this review, we aim to provide a survey of the current landscape of reported indicators. We hope this review will be a useful guide to researchers who are interested in using indicators for biological applications and to tool developers seeking opportunities to create new and improved indicators.

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“…Notably, the incorporation of essential biological elements has been proven to be beneficial for activating the peripheral nervous system ( Liu et al, 2021 ) and regulating bone regeneration ( Li et al, 2020 ). Potassium is one of the most abundant cations in intracellular fluid and plays an essential role in cell function, particularly in excitable cells, such as muscles and nerves ( Fortin et al, 2008 ; Wang et al, 2020 ). The cellular membrane potential can be determined using the potassium gradient across the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Potassium Titanate Assembled Titanium Dioxide Nanotube Arrays Endow Titanium Implants Excellent Osseointegration Performance and Nerve Formation Potential

et al. 2022

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Titanium based materials have been widely applied in bone-tissue engineering. However, inefficient bone repair remains to be solved due to the lack of neural network reconstruction at the bone-implant interface. Herein, we propose a functional surface modification approach to promote neurogenesis. Using an electrochemical technique and a hydrothermal approach, a potassium titanate nanorod-decorated titanium oxide (K2Ti6O13-TiO2) nanotube array is constructed on the surface of titanium implants. The K2Ti6O13-TiO2 hybrid nanotube array on titanium implants can enhance the osteogenic differentiation of mesenchymal stem cells due to the special nanostructures of titanium oxide nanorods. Meanwhile, the release of potassium ions is able to accelerate the neural differentiation of neural stem cells. This study provides a new approach to promote neuralization on the surface of implants, which is promising for future applications in constructing a fully functional interface in bone repair.

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A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells

Abstract: We report a dual-fluorophore approach for ratiometric fluorescent imaging of K+ levels in live cells. Intracellular esterases cleave RPS-1 to detach the K+-responsive fluorophore (PS525) from its internal standard (Coumarin 343).

Cited by 30 publications

References 70 publications

Network topology–directed design of molecular CPU for cell-like dynamic information processing

Network topology–directed design of molecular CPU for cell-like dynamic information processing

Fluorescent Indicators For Biological Imaging of Monatomic Ions

Potassium Titanate Assembled Titanium Dioxide Nanotube Arrays Endow Titanium Implants Excellent Osseointegration Performance and Nerve Formation Potential

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