A dual-response fluorescent probe for simultaneously monitoring polarity and ATP during autophagy

Abstract

Autophagy plays a vital role in maintaining intracellular homeostasis through a lysosome-dependent intracellular degradation pathway, which is closely related to the polarity and ATP. Herein, the first example of the...

Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics

“…For elucidating whether intracellular polarity affects intracellular temperature sensing, we investigated the variation in fluorescence of TPEBT-P­(NIPAM- co -TPSS) with the change in intracellular polarity. The previous report indicated that DMSO can be quickly absorbed by cells to decrease the intracellular polarity Figure S25 showed no significant change in the average fluorescence intensity of HeLa cells treated with DMSO, indicating that fluorescence of TPEBT-P­(NIPAM- co -TPSS) is insensitive to polarity compared with temperature in the real intracellular environment.…”

“…The previous report indicated that DMSO can be quickly absorbed by cells to decrease the intracellular polarity. 27 Figure S25 showed no significant change in the average fluorescence intensity of HeLa cells treated with DMSO, indicating that fluorescence of TPEBT-P(NIPAM-co-TPSS) is insensitive to polarity compared with temperature in the real intracellular environment.…”

Construction of Rhodamine-Based Conjugated Polymer Sensing Platform for Ratiometric Detection of ATP

“…Fluorescence assay has emerged as an attractive technique in the field of biomolecular sensing due to its high sensitivity, selectivity, simplicity, and capability of real-time imaging in biological systems. − Conjugated polymers coordinate many absorbing units and have powerful light-harvesting and signal amplification features, making it a highly sensitive platform for biomolecules detection compared to their small molecule counterparts. − In recent years, several small-molecule-based fluorescent ATP sensors considering electrostatic interactions between phosphates and positively charged recognition groups, covalent bonds between ribose and electronegative atoms, or π–π interactions between adenine and conjugated structures have been fabricated successively (Table S1). − However, polymer-based sensors for ATP detection are still rare, especially for ratiometric methods.…”

NIR Mitochondrial Fluorescent Probe for Visualizing SO2/Polarity in Drug Induced Inflammatory Mice

“…In biological systems, polarity is an important microenvironmental parameter in cells and plays an important role in maintaining a normal biological metabolism . Polarity is closely related to various physiological activities, such as membrane fusion, protein denaturation, conformational changes of enzymes, and peptide aggregation. , Abnormal changes in intracellular polarity cause many diseases, such as diabetes, Alzheimer’s disease, diabetes mellitus, liver cirrhosis, inflammation, and cancer. , At present, polar fluorescent probes have been used to monitor starvation or drug-induced mitotic processes as well as to visualize cellular lipid droplets. , Therefore, real-time detection of intracellular polarity changes is crucial for early diagnosis and preventive treatment of related diseases.…”

“…15 Polarity is closely related to various physiological activities, such as membrane fusion, protein denaturation, conformational changes of enzymes, and peptide aggregation. 16,17 Abnormal changes in intracellular polarity cause many diseases, such as diabetes, Alzheimer's disease, diabetes mellitus, liver cirrhosis, inflammation, and cancer. 18,19 At present, polar fluorescent probes have been used to monitor starvation or drug-induced mitotic processes as well as to visualize cellular lipid droplets.…”