A Dual‐Response Fluorescent Probe Reveals the H₂O₂‐Induced H₂S Biogenesis through a Cystathionine β‐Synthase Pathway

Abstract

The two signaling molecules H2 S and H2 O2 play key roles in maintaining intracellular redox homeostasis. The biological relationship between H2 O2 and H2 S remains largely unknown in redox biology. In this study, we rationally designed and synthesized single- and dual-response fluorescent probes for detecting both H2 O2 and H2 S in living cells. The dual-response probe was shown to be capable of mono- and dual-detection of H2 O2 and H2 S selectively and sensitively. Detailed bioimaging studies based on the probes revealed that both exogenous and endogenous H2 O2 could induce H2 S biogenesis in living cells. By using gene-knockdown techniques with bioimaging, the H2 S biogenesis was found to be majorly cystathionine β-synthase (CBS)-dependent. Our finding shows the first direct evidence on the biological communication between H2 O2 (ROS) and H2 S (RSS) in vivo.

Dual‐Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2S

“…Next, cells were treated with l ‐Cys followed by incubation with 2 , and a strong fluorescence was observed in the Cys‐stimulated HEK293 cells (Figure a). As our study revealed sparfloxacin to be a potent CBS inhibitor, bioimaging of its inhibitory effect was further tested in l ‐Cys‐stimulated HEK293 cells, considering that H 2 S can be produced from l ‐Cys in HEK293 cells via the CBS pathway . The results showed the suppression of l ‐Cys‐induced H 2 S production by sparfloxacin (Figure 6 a, b), implying that sparfloxacin could inhibit endogenous H 2 S biosynthesis.…”

Fast‐Response Turn‐on Fluorescent Probes Based on Thiolysis of NBD Amine for H2S Bioimaging

“…To further demonstrate the biological applicability of the NBD‐based probes, we examined whether they can detect H 2 O 2 ‐induced H 2 S in living cells. In our previous work, the first H 2 O 2 –H 2 S dual‐response probe was prepared for visualization of H 2 O 2 ‐induced H 2 S in living cells for the first time . Here, when cells were treated with different concentrations of H 2 O 2 followed by 1 , fluorescent signals were observed (Figure , Figure S10).…”

A new H2S-specific near-infrared fluorescence-enhanced probe that can visualize the H2S level in colorectal cancer cells in mice

“…Changes of the endogenous H 2 S level in living cells by inhibitors AOAA or siRNA were further performed by the gene-knockdown-based fluorescence imaging strategy from our previous work. 11d,23 Normal cells and specific gene-silencing cells were used for the bioimaging of endogenous H 2 S with probe 1 (Fig. S20†).…”