A dual therapeutic system based on corrole-siRNA conjugates

Abstract

Corrole molecules are a new generation of photosensitizers (PS) due to their ease of tunability for different medical applications. Their ability to initiate cellular death using a wide range of...

Direct Conjugation of Gallium‐(III)‐Corroles to Short Interfering RNA(siRNA) Providing Real‐Time siRNA Imaging and Gene Silencing

“…This is not surprising, because these siRNA modifications did not exhibit any PDT activity in our cytotoxicity assays therefore, we do not expect to observe an increase in potency due to red‐light irradiation. Additionally, this was expected according to our previous work that showed no significant difference between red light and no light treatment of a free base corrole siRNA at the concentrations tested [9] . In conclusion, the results of this experiment demonstrate the ability of the modified sequences to produce potent gene silencing effects comparable to the WT siRNA with no negative effects due to the presence of Ga‐(III)‐corrole modification.…”

“…In our previous study, we explored the direct conjugation of free‐base corrole ligands to the sense strand of siRNAs [9] . This system resulted in a combination therapy that consisted of gene silencing and photodynamic therapy [9] .…”

“…[8b] In our previous study, we explored the direct conjugation of free-base corrole ligands to the sense strand of siRNAs. [9] This system resulted in a combination therapy that consisted of gene silencing and photodynamic therapy. [9] In this research, we are interested in expanding on our previous study and exploring the effect of metalated-corrole ligands to the sense strand of siRNAs.…”

“…[9] This system resulted in a combination therapy that consisted of gene silencing and photodynamic therapy. [9] In this research, we are interested in expanding on our previous study and exploring the effect of metalated-corrole ligands to the sense strand of siRNAs. We hypothesized that the addition of the Ga-(III)-corrole to the siRNA would result in a combination therapy consisting of gene silencing, imaging and potentially an increase in photodynamic therapy.…”

Construction of 5-Fluorouracil and Gallium Corrole Conjugates for Enhanced Photodynamic Therapy

“…In addition, the corrole macrocycle has a direct pyrrole–pyrrole linkage, which makes it more easily metabolized after PDT . Although there have been reports about the synthesis of corrole conjugates for antitumor application such as cyclodextrin, coumarin, amino acid, protein, peptide, and siRNA conjugates, the synthesis of corrole and the chemotherapeutic drug conjugate and its application in chemo-photodynamic combination therapy have not been reported so far.…”