2005
DOI: 10.1074/jbc.m509340200
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A Dual Upstream Open Reading Frame-based Autoregulatory Circuit Controlling Polyamine-responsive Translation

Abstract: A novel form of translational regulation is described for the key polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC). Plant AdoMetDC mRNA 5 leaders contain two highly conserved overlapping upstream open reading frames (uORFs): the 5 tiny and 3 small uORFs. We demonstrate that the small uORF-encoded peptide is responsible for constitutively repressing downstream translation of the AdoMetDC proenzyme ORF in the absence of increased polyamine levels. This first example of a sequence-depen… Show more

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Cited by 137 publications
(141 citation statements)
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“…1 A). In plant mRNAs encoding S-adenosylmethionine decarboxylase, which is another key enzyme in the biosynthesis of polyamines, a short, highly conserved, regulatory, AUG-initiated uORF also ends with the dipeptide PS (16). Data supporting the assertion that the observed conservation near the C terminus of the antizyme inhibitor uCC is at the amino acid level and not at the nucleotide level is provided in SI Text and Fig.…”
Section: Conserved Upstream Coding Region In Homologs Of Odcmentioning
confidence: 51%
See 1 more Smart Citation
“…1 A). In plant mRNAs encoding S-adenosylmethionine decarboxylase, which is another key enzyme in the biosynthesis of polyamines, a short, highly conserved, regulatory, AUG-initiated uORF also ends with the dipeptide PS (16). Data supporting the assertion that the observed conservation near the C terminus of the antizyme inhibitor uCC is at the amino acid level and not at the nucleotide level is provided in SI Text and Fig.…”
Section: Conserved Upstream Coding Region In Homologs Of Odcmentioning
confidence: 51%
“…The comparable situation in plant S-adenosylmethionine decarboxylase 5Ј leaders, which have evolved independently from vertebrates, may be even more relevant because initiation of the first of two highly conserved uORFs may be influenced by polyamine-dependent leaky scanning (16). Yet another example of translational autoregulation in genes influencing polyamine synthesis is the programmed ribosomal frameshifting required for antizyme synthesis acting as a sensor of polyamine levels (34,35).…”
Section: Discussionmentioning
confidence: 99%
“…The net accumulation of GRIK1 and GRIK2 most likely reflects a balance between these two processes. Interestingly, the 5#-untranslated regions of both GRIK1 and GRIK2 mRNAs contain multiple upstream small open reading frames (data not shown), which have been associated with translational regulation of other mRNAs (Morris and Geballe, 2000;Wiese et al, 2004;Hanfrey et al, 2005). AL1 binding to GRIK1 and GRIK2 may protect them from degradation.…”
Section: Discussionmentioning
confidence: 99%
“…The polyamine biosynthetic and catabolic enzymes are tightly regulated in animals, plants, and yeast (3,4,24). Unusually, in the trypanosomatid parasites, analogous regulatory mechanisms for the control of polyamine biosynthesis have not been identified.…”
mentioning
confidence: 99%