A dye combination for the staining of pollen coat and pollen wall

Jia, Xin‐Lei; Xue, Jing-Shi; Zhang, Fang; Yao, Cheng; Shen, Shiyi; Sui, Chang-Xu; Peng, Yu-Jia; Xu, Qin-Lin; Feng, Yi-Feng; Hu, Wenjing; Xu, Ping; Yang, Zhong‐Nan

doi:10.1007/s00497-021-00412-5

Cited by 16 publications

(12 citation statements)

References 41 publications

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“…B0904, Sigma) stains the phenylpropanoid phenolics. We showed the expression of these pathway genes in tapetum (Xue et al, 2020), and this dye also stains the sporopollenin wall (Jia et al, 2021). Furthermore, these two dyes show non-overlapped emission wavelengths.…”

Section: Chemicalsmentioning

confidence: 76%

Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida

et al. 2022

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Reconstructing the development of sporangia in seed-free vascular plants provides crucial information about key processes enabling the production of spores that are important in the life cycle of these plants. By applying fluorescence imaging in intact tissues using dyes and confocal microscopy, this study aimed to reconstruct the key steps during the development of sporangia. Special emphasis was taken on the cell wall structures of tapetum and spore mother cells that have been challenged by microscopical documentation in the past. After staining the cell wall and cytoplasm using calcofluor white and basic fuchsin, the sporangium development of Pteris multifida was observed using confocal microscopy. The clear cell lineages from the sporangial initial cell to stalk, epidermis, inner tapetum, outer tapetum, and sporogenous cells were revealed by confocal imaging. The sporangium development improved in this work will be useful for a general understanding of fern spore formation.

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Section: Chemicalsmentioning

confidence: 76%

Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida

et al. 2022

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“…The membrane transporters AtCHX17, AtCHX18, and AtCHX19 regulate K + and pH homeostasis, and a triple mutant of these genes exhibits disordered reticulate morphology of pollen walls, indicating a defect in pollen wall formation during male gametophyte development [ 72 ] The walls of the pollen first touch the stigma of the pistil during pollination to determine if the pollen is adhering to the stigma [ 73 ]. The pollen wall is implicated in pollen adhesion, hydration, germination, pollen–stigma recognition and interactions [ 74 ], and the polar growth of the pollen tube [ 72 ] Therefore, we speculate that CHX18 may affect the hydration of pollen and the pollen–stigma interaction by altering pollen wall formation to affect self-incompatibility.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa

Yang

Liu

et al. 2023

Cells

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Heavy metal-associated proteins (HMPs) participate in heavy metal detoxification. Although HMPs have been identified in several plants, no studies to date have identified the HMPs in Brassica rapa (B. rapa). Here, we identified 85 potential HMPs in B. rapa by bioinformatic methods. The promoters of the identified genes contain many elements associated with stress responses, including response to abscisic acid, low-temperature, and methyl jasmonate. The expression levels of BrHMP14, BrHMP16, BrHMP32, BrHMP41, and BrHMP42 were upregulated under Cu2+, Cd2+, Zn2+, and Pb2+ stresses. BrHMP06, BrHMP30, and BrHMP41 were also significantly upregulated after drought treatment. The transcripts of BrHMP06 and BrHMP11 increased mostly under cold stress. After applying salt stress, the expression of BrHMP02, BrHMP16, and BrHMP78 was induced. We observed increased BrHMP36 expression during the self-incompatibility (SI) response and decreased expression in the compatible pollination (CP) response during pollen–stigma interactions. These changes in expression suggest functions for these genes in HMPs include participating in heavy metal transport, detoxification, and response to abiotic stresses, with the potential for functions in sexual reproduction. We found potential co-functional partners of these key players by protein–protein interaction (PPI) analysis and found that some of the predicted protein partners are known to be involved in corresponding stress responses. Finally, phosphorylation investigation revealed many phosphorylation sites in BrHMPs, suggesting post-translational modification may occur during the BrHMP-mediated stress response. This comprehensive analysis provides important clues for the study of the molecular mechanisms of BrHMP genes in B. rapa, especially for abiotic stress and pollen–stigma interactions.

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“…For SEM, after fixation with FAA fixative, the anthers were dehydrated to 100% alcohol by gradient, treated by a critical point drying method, coated with 8 nm gold and observed under a JSM‐840 microscope. Pollen wall staining was performed as previously described (Jia et al., 2021). The pollen exine was stained with basic fuchsin solution, while the pollen intine was stained with calcofluor white 28 solution.…”

Section: Methodsmentioning

confidence: 99%

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

Zhang,

Guo,

Wang

et al. 2024

The Plant Journal

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SUMMARYThermosensitive genic female sterility (TGFS) is a promising property to be utilized for hybrid breeding. Here, we identified a rice TGFS line, tfs2, through an ethyl methyl sulfone (EMS) mutagenesis strategy. This line showed sterility under high temperature and became fertile under low temperature. Few seeds were produced when the tfs2 stigma was pollinated, indicating that tfs2 is female sterile. Gene cloning and genetic complementation showed that a point mutation from leucine to phenylalanine in HEI10 (HEI10tfs2), a crossover formation protein, caused the TGFS trait of tfs2. Under high temperature, abnormal univalents were formed, and the chromosomes were unequally segregated during meiosis, similar to the reported meiotic defects in oshei10. Under low temperature, the number of univalents was largely reduced, and the chromosomes segregated equally, suggesting that crossover formation was restored in tfs2. Yeast two‐hybrid assays showed that HEI10 interacted with two putative protein degradation‐related proteins, RPT4 and SRFP1. Through transient expression in tobacco leaves, HEI10 were found to spontaneously aggregate into dot‐like foci in the nucleus under high temperature, but HEI10tfs2 failed to aggregate. In contrast, low temperature promoted HEI10tfs2 aggregation. This result suggests that protein aggregation at the crossover position contributes to the fertility restoration of tfs2 under low temperature. In addition, RPT4 and SRFP1 also aggregated into dot‐like foci, and these aggregations depend on the presence of HEI10. These findings reveal a novel mechanism of fertility restoration and facilitate further understanding of HEI10 in meiotic crossover formation.

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A dye combination for the staining of pollen coat and pollen wall

Cited by 16 publications

References 41 publications

Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida

Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida

Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

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