A facile enzyme-assisted multiple recycling amplification strategy for ultrasensitive fluorescence detection of HIV-1 DNA

Label-free DNA quantification using isothermal amplification on an exposed core optical fiber microfluidic platform

“…23,24 In the above isothermal amplification methods, as for traditional qPCR, DNA is detected and quantified by monitoring a change in fluorescence intensity. [25][26][27][28][29] This requires fluorescent labelling reagents as well as a fluorescence excitation and detection device, which increases the complexity and is not favorable to the needs of on-site detection.…”