A facile large-scale microwave synthesis of highly fluorescent carbon dots from benzenediol isomers

Wang, Jun; Cheng, C.S. Agnes; Zhang, Shuai; Zheng, Baozhan; Yuan, Huatang; Lin, Bo; Zheng, Mingwu; Yang, Shengyong; Guo, Yong; Xiao, Dan

doi:10.1039/c3tc32131b

Cited by 84 publications

(46 citation statements)

References 53 publications

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“…The UV−vis absorption spectrum of CNDs showed typically absorption peaks at 228 and 283 nm, which was attributed to the π–π* transition of CC, and n–π* transition of CO, respectively. These features were consistent with previously reported CNDs . Photoluminescent excitation spectra displayed a prominent peak at 370 nm.…”

Section: Resultssupporting

confidence: 92%

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

Jiang

et al. 2016

Part & Part Syst Charact

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1 of 7) 1600197Temperature measurement in biology and medical diagnostics is of great importance. Herein, a novel carbon nanodot (CND) based fluorescent nanothermometry device for spatially resolved temperature measurements is demonstrated. The fluorescence CNDs are prepared by a simple one-pot solvothermal method using sucrose as carbon source. Resultant CNDs show stable green fluorescence at 520 nm with high quantum yield (≈6%). The fluorescence of resultant CNDs exhibits a reversible linear response to temperature in a wide range of 20-85 °C. Moreover, the temperature resolution better than 0.5 °C and high sensitive variation of ≈1.3% °C −1 are observed in a broad physiological temperature range of 20-40 °C. Therefore, the as-prepared CNDs possess high water solubility, stable fluorescence, small size, and good biocompatibility, which make them promising candidate for thermometry and cell imaging in biological media. stability, serious toxicity, and high cost, have limited their further application.The carbon nanodots (CNDs), a new family member of carbon nanomaterials, are generally composed of sp 2 hybridized carbon atoms with abundant oxygenous and hydrogenous residues with sizes below 10 nm. [10,11] Compared to the conventional fluorescent nanomaterials (SQDs, metal nanoclusters, and organic fluorescent dyes), CNDs possess numerous superior features including easy preparation and functionalization, no/low toxicity, and high photostability, thus demonstrating a variety of potential applications, such as sensing, biomedicine, catalysis, and optoelectronic devices. [12][13][14][15] To date, many efforts have been done to study the preparations, properties, and applications of CNDs, but most of them show intense emission only in the blue-light region, and the long-wavelength (i.e., green-, yellow-, to red-light) emissions are usually very weak. [16][17][18] Such a drawback restricts further applications of CNDs, particularly in the biology-relevant fields because of the common blue autofluorescence of biological matrix and photodamage of biological tissues by ultraviolet excitation light. Furthermore, many CNDs-based nanosensors have been designed and played an important role in bio-analysts in vitro and vivo. [19][20][21][22] For example, Zhu et al. reported a facile and high output strategy for the fabrication of highly photoluminescent CNDs which could be utilized as a biosensor reagent for the detection of Fe 3+ in bio-systems; [19] Zhang group reported an effective fluorescent probe for sensing mercuric and iodide ions based on the nitrogen-doped CNDs; [20] Nie et al. applied the CNDs as an internal reference to construct a ratiometric pH sensor in living cells, [21] while few CNDs-based thermometers are reported. [23][24][25][26] More recently, our group used a hydrothermal route to prepare water-stable luminescent CNDs as nanosensors for the detection of pH and temperature; [23] then we also designed a "turn-on" chemosensor for detecting temperature through surface-modified CNDs and the carbon-dot-based...

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Section: Resultssupporting

confidence: 92%

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

Jiang

et al. 2016

Part & Part Syst Charact

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“…Some of the methodologies required delicate instrumentation or are tedious in procedures or harmful chemical reagents are unavoidable, making the synthesis technically unfeasible to perform in general laboratories. Among all the available protocols, microwave-assisted methods are efficient, time-saving, cost-effective, environment-friendly, and narrow size-distributed through the homogeneous heating [21,22]. Therefore, microwave-assisted heating was employed in the synthetic procedure, which was attempted to provide a facile and feasible route of preparing carbon dots without surface passivation or heteroatom doping.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of fluorescent carbon dots via microwave carbonization of citric acid in presence of tetraoctylammonium ion, and their application to cellular bioimaging

et al. 2015

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A jelly-like form of carbon dots (C-dots) was prepared by microwave-assisted synthesis from citric acid in the presence of tetraoctylammonium bromide. The effect of the concentration of tetraoctylammonium bromide was examined. The synthesized carbon dots were characterized by UV-vis, XRD, FTIR, fluorescence and HR-TEM. Fluorescence extends from 350 to 600 nm, and the corresponding excitation wavelengths range from 300 to 460 nm. Quantum yields are at around 0.11. A cytotoxicity study showed carbon dots to be cell permeable and biocompatible which renders them appropriate for imaging applications. The dots were used to image HeLa cell lines via the blue fluorescence of the dots.

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“…In view of the outstanding properties, CDs have been widely used in bioimaging, biosensing, photocatalysis and drug/gene delivery 33–38 . However, only a few fluorescent assays based on CDs have been reported for PPi or ALP activity detection.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Geng

Zhang

et al. 2017

Sci Rep

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In this report, a novel fluorescent sensing platform using nitrogen-doped carbon dots (N-CDs) as probes for fluorescence signal transmission has been designed for the detection of significant biomolecules pyrophosphate (PPi) and alkaline phosphatase (ALP). The high fluorescent N-CDs could be selectively quenched by Cu2+, and recovered by the addition of PPi because PPi preferentially binds to Cu2+. Once ALP was introduced into the system, ALP can specifically hydrolyze PPi into Pi, the intense fluorescence of N-CDs could be quenched again due to the recombination of the as-released Cu2+ with N-CDs. So, fluorescence of N-CDs is regulated by an ALP-triggered reaction. Based on this strategy, we demonstrated that N-CDs could serve as a very effective fluorescent sensing platform for label-free, sensitive and selective detection of PPi and ALP with low detection limit of 0.16 μM and 0.4 U/L for PPi and ALP, respectively. Moreover, the assay time is just around 0.5 min for PPi and 30 min for ALP. This developed strategy shows remarkable advantages including sensitive, rapid, simple, convenient, and low-cost and so forth. Furthermore, this method was also successfully applied to monitor ALP in human serum, which indicates its great potential for practical applications in biological and clinical diagnosis.

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A facile large-scale microwave synthesis of highly fluorescent carbon dots from benzenediol isomers

Abstract: Three different carbon dots with high quantum yields were prepared via microwave method within 2 min. The hydroquinone-derived CDs could easily penetrate cells in 30 min.

Cited by 84 publications

References 53 publications

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

Synthesis of fluorescent carbon dots via microwave carbonization of citric acid in presence of tetraoctylammonium ion, and their application to cellular bioimaging

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

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