A facile low-cost enzymatic paper-based assay for the determination of urine creatinine

Talalak, Kwanrutai; Noiphung, Julaluk; Songjaroen, Temsiri; Chailapakul, Orawon; Laiwattanapaisal, Wanida

doi:10.1016/j.talanta.2015.07.040

Cited by 55 publications

(29 citation statements)

References 42 publications

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“…Paper-based analytical devices (PADs) as demonstrated by Whitesides's group 24 offer a promising solution for point-of-care diagnostics 25 - 27 . PADs can provide potentially relevant analysis for biomarker detection in many types of biological fluids, including tear fluids 28 , 29 , whole blood 30 - 32 , serum 33 , urine 34 and saliva 35 - 37 . To date, the development of PADs for saliva analysis have been reported for biomarkers such as nitrite 38 , thiocyanate 36 and aldehydes 37 using colorimetric assay and dengue specific antibodies using stacked flow immunoassay 35 .…”

Section: Introductionmentioning

confidence: 99%

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Noiphung¹,

Nguyen²,

Punyadeera³

et al. 2018

Theranostics

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Rationale: Saliva as a sample matrix is rapidly gaining interest for disease diagnosis and point-of-care assays because it is easy to collect (non-invasive) and contains many health-related biomarkers. However, saliva poses particular problems relative to more common urine and blood matrices, which includes low analyte concentrations, lack of understanding of biomolecule transportation and inherent viscosity variability in human samples. While several studies have sought to improve assay sensitivity, few have addressed sample viscosity specifically. The goal of this study is to minimize the effect of sample viscosity on paper-based analytical devices (PADs) for the measurement of pH and nitrite in human saliva.Methods: PADs were used to measure salivary pH from 5.0 to 10.0 with a universal indicator consisting of chlorophenol red, phenol red and phenolphthalein. Nitrite determination was performed using the Griess reaction. Artificial saliva with viscosity values between 1.54 and 5.10 mPa∙s was tested on the proposed PAD. To ensure the proposed PADs can be tailored for use in-field analysis, the devices were shipped to Australia and tested with human specimens.Results: Initial experiments showed that viscosity had a significant impact on the calibration curve for nitrite; however, a more consistent curve could be generated when buffer was added after the sample, irrespective of sample viscosity. The linear range for nitrite detection was 0.1 to 2.4 mg/dL using the improved method. The nitrite measurement in artificial saliva also showed a good correlation with the standard spectrophotometry method (p=0.8484, paired sample t-test, n=20). Measured pH values from samples with varying viscosities correlated well with the results from our pH meter.Conclusions: The inherent variation of salivary viscosity that impacts nitrite and pH results can be addressed using a simple washing step on the PAD without the need for complex procedures.

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Section: Introductionmentioning

confidence: 99%

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Noiphung¹,

Nguyen²,

Punyadeera³

et al. 2018

Theranostics

Self Cite

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“…Several other efforts have also prepared fully autonomous paper-based analytical devices for blood analysis, 154−158 and urinalysis. 159−163…”

Section: Paper Diagnostics For Ehealth Applicationsmentioning

confidence: 99%

From Point-of-Care Testing to eHealth Diagnostic Devices (eDiagnostics)

2018

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Point-of-care devices were originally designed to allow medical testing at or near the point of care by health-care professionals. Some point-of-care devices allow medical self-testing at home but cannot fully cover the growing diagnostic needs of eHealth systems that are under development in many countries. A number of easy-to-use, network-connected diagnostic devices for self-testing are needed to allow remote monitoring of patients’ health. This Outlook highlights the essential characteristics of diagnostic devices for eHealth settings and indicates point-of-care technologies that may lead to the development of new devices. It also describes the most representative examples of simple-to-use, point-of-care devices that have been used for analysis of untreated biological samples.

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“…cancer, non‐communicable diseases, and metabolic disorders) relating to current life‐styles of the world population. Tables and describe the target biomarkers, types of paper substrates, fabrication, and detection techniques, analytical performance characteristics with their respective applications in real sample matrices, for tumor marker and other diseased‐related biomarker detection, respectively. Highlights of selected examples are discussed.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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A facile low-cost enzymatic paper-based assay for the determination of urine creatinine

Cited by 55 publications

References 42 publications

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

From Point-of-Care Testing to eHealth Diagnostic Devices (eDiagnostics)

Recent applications of paper‐based point‐of‐care devices for biomarker detection

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