A facile one-pot method to high-quality Ag-graphene composite nanosheets for efficient surface-enhanced Raman scattering

Zhang, Zhe; Xu, Fugang; Yang, Wenshu; Guo, Mingyi; Wang, Xiaodan; Zhang, Bailin; Tang, Jilin

doi:10.1039/c1cc11125f

Cited by 255 publications

(162 citation statements)

References 33 publications

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“…As shown in Figure 2D, the nanocomposites exhibited two characteristic peaks at 25.88° and 32.8°, corresponding to rGO and AgNPs, which indicated that the nanocomposites contains both nanomaterials in a single platform and also confirming that Ag existed in a crystalline state. 21,28 The purity of peaks represented by graphene and Ag clearly indicated the reduction of GO and Ag ions, and suggested that the rGO-AgNP nanocomposite lattice was unaffected by other molecules in C-phycocyanin.…”

Section: 35mentioning

confidence: 99%

“…26 In addition, graphene offers a suitable platform for the development of nanocomposites, allowing the combination of nanomaterials with different properties to give novel materials improved or new functionality. Particularly, GO is used for the attachment of AgNPs, 21,27,28 due to its high surface area that serves as a support for growth and stabilization of NPs, which prevents them from aggregating. 29 However, studies related to anticancer activity of GO-silver nanocomposites have been very limited.…”

Section: Introductionmentioning

confidence: 99%

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Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Yuan¹,

Gurunathan²

2017

IJN

116

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Background Cisplatin (Cis) is a widely used chemotherapeutic drug for treating a variety of cancers, due to its ability to induce cell death in cancer cells significantly. Recently, graphene and its modified nanocomposites have gained much interest in cancer therapy, due to their unique physicochemical properties. The objective of this study was to investigate the combination effect of Cis and a reduced graphene oxide–silver nanoparticle nanocomposite (rGO-AgNPs) in human cervical cancer (HeLa) cells. Materials and methods We synthesized AgNPs, rGO, and rGO-AgNP nanocomposites using C-phycocyanin. The synthesized nanomaterials were characterized using various analytical techniques. The anticancer properties of the Cis, rGO-AgNPs, and combination of Cis and rGO-AgNPs were evaluated using a series of cellular assays, such as cell viability, cell proliferation, LDH leakage, reactive oxygen species generation, and cellular levels of oxidative and antioxidative stress markers such as malondialdehyde, glutathione, SOD, and CAT. The expression of proapoptotic, antiapoptotic, and autophagy genes were measured using real-time reverse-transcription polymerase chain reaction. Results The synthesized AgNPs were well dispersed, homogeneous, and spherical, with an average size of 10 nm and uniformly distributed on graphene sheets. Cis, GO, rGO, AgNPs, and rGO-AgNPs inhibited cell viability in a dose-dependent manner. The combination of Cis and rGO-AgNPs showed significant effects on cell proliferation, cytotoxicity, and apoptosis. The combination of Cis and rGO-AgNPs had more pronounced effects on the expression of apoptotic and autophagy genes, and also significantly induced the accumulation of autophagosomes and autophagolysosomes, which was associated with the generation of reactive oxygen species. Conclusion Our findings substantiated rGO-AgNPs strongly potentiating Cis-induced cytotoxicity, apoptosis, and autophagy in HeLa cells, and hence rGO-AgNPs could be potentially applied to cervical cancer treatment as a powerful synergistic agent with Cis or any other chemotherapeutic agents.

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Section: 35mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Yuan¹,

Gurunathan²

2017

IJN

116

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“…High quality single-layer HRG/Ag nanocomposites, which were used as SERS substrate for the detection of 4-aminothiophenol in liquid, were obtained using poly-vinyl-2-pyrrolidone (PVP) as the reductant and stabilizer. 574 Similarly, poly(diallyldimethyl ammonium chloride (PDDA) was used stabilizer for preparing cationic polyelectrolyte functionalized graphene oxide and as support for Ag NPs for detecting folic acid by SERS. 575 Apart from silver, Au NPs (like Ag NPs) induce a large enhancement of the Raman signal when deposited on graphene.…”

Section: Surface-enhanced Raman Scattering Substratesmentioning

confidence: 99%

Graphene based metal and metal oxide nanocomposites: synthesis, properties and their applications

et al. 2015

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“…[30] Furthermore, UV spectrum of GO with AgNPs exhibits the peak at 422 nm from the surface plasmon band of AgNPs. [29,31] Hence, these results suggest that AgNPs have successfully labeled on the GO, and then it would be used as an enhanced agent of CT imaging.…”

Section: Preparation and Characterization Of Go/agnpsmentioning

confidence: 70%

Graphene Oxide/Ag Nanoparticles Cooperated with Simvastatin as a High Sensitive X‐Ray Computed Tomography Imaging Agent for Diagnosis of Renal Dysfunctions

Zhan

Tian

Liu

et al. 2017

Adv Healthcare Materials

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Graphene oxides (GO) are attracting much attention in the diagnosis and therapy of the subcutaneous tumor as a novel biomaterial, but its diagnosis to tissue dysfunction is yet to be found. Here, a novel application of GO for diagnosis of renal dysfunction via contrast‐enhanced computed tomography (CT) is proposed. In order to serve as contrast‐enhanced agent, Ag nanoparticles (AgNPs) are composited on the surface of GO to promote its X‐ray absorption, and then simvastatin is coinjected for eliminating in vivo toxicity induced by AgNPs. It is found that GO/AgNPs can enhance the imaging of CT into the lung, liver, and kidney of mice for a long circulation time (≈24 h) and a safety profile in vivo in the presence of simvastatin. Interestingly, the lower dose of GO/AgNPs (≈0.5 mg per kg bw) shows an excellent performance for CT imaging of renal perfusion, and visually exhibits the right renal dysfunction in model mice. Hence, this work suggests that graphene nanoparticles will play a vital role for the future medical translational development including drug carrier, biosensing, and disease therapy.

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A facile one-pot method to high-quality Ag-graphene composite nanosheets for efficient surface-enhanced Raman scattering

Cited by 255 publications

References 33 publications

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Graphene based metal and metal oxide nanocomposites: synthesis, properties and their applications

Graphene Oxide/Ag Nanoparticles Cooperated with Simvastatin as a High Sensitive X‐Ray Computed Tomography Imaging Agent for Diagnosis of Renal Dysfunctions

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A facile one-pot method to high-quality Ag-graphene composite nanosheets for efficient surface-enhanced Raman scattering

Cited by 255 publications

References 33 publications

Combination of graphene oxide&ndash;silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Combination of graphene oxide&ndash;silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Graphene based metal and metal oxide nanocomposites: synthesis, properties and their applications

Graphene Oxide/Ag Nanoparticles Cooperated with Simvastatin as a High Sensitive X‐Ray Computed Tomography Imaging Agent for Diagnosis of Renal Dysfunctions

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Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells