A Fast and Inexpensive Procedure for Drying Polyacrylamide Gels

Fadouloglou, Vasiliki E.; Glykos, Nicholas M.; Kokkinidis, Michael

doi:10.1006/abio.2000.4852

Cited by 7 publications

(6 citation statements)

References 3 publications

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“…To understand the reversible swelling mechanism of polyacrylamide-bisacrylamide hydrogels in ethanol, it is important to know why these hydrogels have a sensitivity to ethanol. Fadouloglou et al used ethanol to dry out polyacrylamide hydrogels, which are the standard system for gel electrophoresis of proteins, reproducibly after electrophoresis and observed a uniform shrinking of the gel after 20 min in 95 vol% ethanol (Fadouloglou et al, 2000). However, the authors have not given any explanation for this effect.…”

Section: Reversibility Of Hydrogel Swellingmentioning

confidence: 99%

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Hydrogel-based piezoresistive sensor for the detection of ethanol

Erfkamp

Guenther

Gerlach

2018

J. Sens. Sens. Syst.

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Abstract. This article describes a low-cost sensor for the detection of ethanol in alcoholic beverages, which combines alcohol-sensitive hydrogels based on acrylamide and bisacrylamide and piezoresistive sensors. For reproducible measurements, the reversible swelling and deswelling of the hydrogel were shown via microscopy. The response time of the sensor depends on the swelling kinetics of the hydrogel. The selectivity of the hydrogel was tested in different alcohols. In order to understand the influence of monomer and crosslinker content on the swelling degree and on the sensitivity of the hydrogels, gels with variable concentrations of acrylamide and bisacrylamide were synthesized and characterized in different aqueous solutions with alcohol contents. The first measurements of such hydrogel-based piezoresistive ethanol sensors demonstrated a high sensitivity and a short response time over several measuring cycles.

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Section: Reversibility Of Hydrogel Swellingmentioning

confidence: 99%

“…As expected, the response time of the sensors amounting to a few minutes is shorter than of hydrogels in free-swelling mode. The response times of the sensors could be further reduced by using porous hydrogels (Franke et al, 2017).…”

Section: Piezoresistive Ethanol Sensormentioning

confidence: 99%

Hydrogel-based piezoresistive sensor for the detection of ethanol

Erfkamp

Guenther

Gerlach

2018

J. Sens. Sens. Syst.

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“…14,15) Briefly, after treatment with asiatic acid, SW480 cells were lysed and suspended in Trisethylenediaminetetraacetic acid (EDTA) buffer (pH 7.5). Precipitation solution was added, mixed and each sample was placed on ice for 15 min.…”

Section: Asiatic Acid Induces Colon Cancer Cell Growth Inhibition Andmentioning

confidence: 99%

Asiatic Acid Induces Colon Cancer Cell Growth Inhibition and Apoptosis through Mitochondrial Death Cascade

Tang

Xia

Jung

et al. 2009

Biological & Pharmaceutical Bulletin

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Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis.

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“…Reduced and denatured samples were loaded onto NuPAGE s Novex s 4-12% Bis-Tris polyacrylamide gels (Life Technologies) and electrophoresed at constant voltage in 1 Â Novex s 2-(N-morpholino)ethanesulfonic acid running buffer (MES; 145 V) (Life Technologies) or 1 Â Novex s 3-(N-morpholino)propanesulfonic acid running buffer (MOPS; 125 V) (Life Technologies) supplemented with NuPAGE s antioxidant (Life Technologies) per manufacturer's instructions. PAGE separated gels were incubated in gel drying buffer [50% methanol (HPLC grade; Honeywell), 7.5% glacial acetic acid (Fisher Chemical), and 10% glycerol (ACS grade; Macron)] for 30 min at room temperature (RT), further dehydrated in 200 proof ethanol (ETOH, Decon Laboratories) for 15 min at RT (Fadouloglou et al, 2000), and then submerged in Amersham™ Amplify™ fluorographic reagent (GE Healthcare) for 30 min at RT to infuse the gel with the fluorographic reagent. PAGE gels were dried completely under vacuum at 80 1C for 2 h. PAGE separated [35] Scysteine labeled products were imaged using BIOMAX MR HighResolution Film (Carestream).…”

Section: Polyacrylamide Gel Electrophoresismentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 (nsp2) topology and selective isoform integration in artificial membranes

2015

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The membrane insertion and topology of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) strain VR-2332 was assessed using a cell free translation system in the presence or absence of artificial membranes. Expression of PRRSV nsp2 in the absence of all other viral factors resulted in the genesis of both full-length nsp2 as well as a select number of C-terminal nsp2 isoforms. Addition of membranes to the translation stabilized the translation reaction, resulting in predominantly full-length nsp2 as assessed by immunoprecipitation. Analysis further showed full-length nsp2 strongly associates with membranes, along with two additional large nsp2 isoforms. Membrane integration of full-length nsp2 was confirmed through high-speed density fractionation, protection from protease digestion, and immunoprecipitation. The results demonstrated that nsp2 integrated into the membranes with an unexpected topology, where the amino (N)-terminal (cytoplasmic) and C-terminal (luminal) domains were orientated on opposite sides of the membrane surface.

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A Fast and Inexpensive Procedure for Drying Polyacrylamide Gels

Cited by 7 publications

References 3 publications

Hydrogel-based piezoresistive sensor for the detection of ethanol

Hydrogel-based piezoresistive sensor for the detection of ethanol

Asiatic Acid Induces Colon Cancer Cell Growth Inhibition and Apoptosis through Mitochondrial Death Cascade

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 (nsp2) topology and selective isoform integration in artificial membranes

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