A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations

Abstract

A simple, fast, and validated HPLC method was developed for the simultaneous quantization of five cardiovascular agents: dopamine (DPM), dobutamine (DBM), phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion samples or in an injection dosage form. The proposed method was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column, by using a simple linear gradient. Mobile phase A was buffer (50 mM KH2PO4) and mobile Phase B was acetonitrile at a flow rate of 1.0 mL/min. The column temperature was kept at 30°C, and the injection volume was 20 μL. All analytes were separated simultaneously at a retention time (tr) of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, FSM, and APL, respectively, with a total run time of less than 15.0 min. The proposed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. Linearity was obtained over a concentration range of 12.0–240.0, 12.0–240.0, 20.0–200.0, 6.0–240.0, and 10.0–200.0 μg/mL DPM, DBM, PTM, FSM, and APL, respectively. Interday and intraday accuracy and precision data were recorded in the acceptable limits. The new method has successfully been applied for quantification of all five drugs in their injection dosage form, infusion samples, and for evaluation of the stability of investigated drugs in mixtures for endovenous use. The results of the stability study showed that mixtures of DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were stable for 48 hours when stored in polypropylene syringes at 25°C.

The Application of Dopamine Combined with Intravenous Furosemide Infusion Therapy Has an Apparent Clinical Effect in Treating Patients with Heart Failure

“…The progression of the condition and the use of other antihypertensive medications may further impair renal function, resulting in efficacy and diuretics [ 12 , 13 ]. Moreover, dopamine is the most abundant neurotransmitter in the brain, which regulates various physiological functions of the central nervous system as a neurotransmitter [ 14 , 15 ]. Therefore, the use of diuretics to relieve the symptoms of patients with nephrogenic edema and cirrhosis and the addition of dopamine to stabilize the patient's cardiac function to meet the patient's therapeutic needs and enhance the efficacy are mostly recommended in clinical practice [ 16 ].…”

A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

“…The limit of detection for each 5-HT 3 receptor antagonist was determined at a signal-to-noise ratio (S/N) of 3. For the limit of quantification, the ratio considered was 10 : 1 with an RSD% value less than 10% [ 34 ].…”

Ultrasensitive and Simple Dopamine Electrochemical Sensor Based on the Synergistic Effect of Cu-TCPP Frameworks and Graphene Nanosheets

“…In recent years, researchers have made a great deal of effort around the development of high sensitivity and selectivity determination techniques of DA. Spectrometric techniques [ 4 ], high-performance liquid chromatography–diode array (HPLC-DA) [ 5 ], fluorometry [ 6 ], and chemiluminescence method [ 7 ] have all been reported for the detection of DA. However, most of these methods are complicated to operate, high cost, and time consuming.…”