A Fast Colourimetric Assay for Lead Detection Using Label-Free Gold Nanoparticles (AuNPs)

Zhong, Guowei; Liu, Jinxia; Liu, Xinyu

doi:10.3390/mi6040462

Cited by 28 publications

(18 citation statements)

References 37 publications

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“…This binding changes the orientation of the terminal polar Glu and Gly residues and exposes them to the anionic carboxylic acids which assist in the solubility and stability of the NPs in water. Cysteine possesses a thiol group (-SH) that is characterized by a high affinity to gold and, consequently, an Au-S covalent bond is formed 27 . In parallel, intermolecular hydrogen bonds are formed among GSH molecules, even during their adsorption to the surfaces of the gold nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

“…In parallel, intermolecular hydrogen bonds are formed among GSH molecules, even during their adsorption to the surfaces of the gold nanoparticles. Hence, these combined molecular mechanisms lead to the aggregation of AuNPs on GSH molecules at certain conditions 27 . The second step includes the adjustment of pH to 8, while the third step includes the formation of AuNPs by the addition of NaBH 4 .…”

Section: Resultsmentioning

confidence: 99%

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Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Sulaiman

Waheeb

Jabir

et al. 2020

Sci Rep

191

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Hesperidin is a flavonoid glycoside with proven therapeutic activities for various diseases, including cancer. However, its poor solubility and bioavailability render it only slightly absorbed, requiring a delivery system to reach its therapeutic target. Hesperidin loaded on gold nanoparticles (Hsp-AuNPs) was prepared by a chemical synthesis method. Various characterization techniques such as UV-VIS spectroscopy, ftiR, XRD, feSeM, teM and eDX, Zeta potential analysis, particle size analysis, were used to confirm the synthesis of Hsp-AuNPs. The cytotoxic effect of Hsp-AuNPs on human breast cancer cell line (MDA-MB-231) was assessed using MTT and crystal violet assays. The results revealed significant decrease in proliferation and inhibition of growth of the treated cells when compared with human normal breast epithelial cell line (HBL-100). Determination of apoptosis by fluorescence microscope was also performed using acridine orange-propidium iodide dual staining assay. The in vivo study was designed to evaluate the toxicity of Hsp-AuNPs in mice. The levels of hepatic and kidney functionality markers were assessed. No significant statistical differences were found for the tested indicators. Histological images of liver, spleen, lung and kidney showed no apparent damages and histopathological abnormalities after treatment with Hsp-AuNPs. Hsp-AuNPs ameliorated the functional activity of macrophages against Ehrlich ascites tumor cells-bearing mice. The production of the pro-inflammatory cytokines was also assessed in bone marrow-derived macrophage cells treated with Hsp-AuNPs. The results obviously demonstrated that Hsp-AuNPs treatment significantly inhibited the secretion of IL-1β, IL-6 and TNF. The burden caused by cancer to the global public health has been markedly increasing, as evidenced by an estimate of 9.6 million mortalities in 2018 (around one sixth of the overall worldwide mortalities), around 70% of which being recorded in nations with low to middle income 1,2. The most frequently diagnosed type of cancer in females is breast cancer, being the reason for more than one tenth of annual newly diagnosed cases and the second cause of mortality. In Iraq, breast cancer occupies the second rank, following cardiovascular diseases, as a major cause of mortality (23% of cancer-related deaths in women) 3. Direct administration of the available chemical drugs is generally problematic, while these chemicals often exert toxicity and unwanted side effects to the patient's

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Sulaiman

Waheeb

Jabir

et al. 2020

Sci Rep

191

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“…The absorbance of the colloidal Ag nanoparticle did not change appreciably upon addition of Pb 2+ , even when Pb 2+ was as high as 10 mg/L. Previous reports showed that colloidal AuNPs capped with L-glutathione [27] did not change the absorbance significantly when a solution of transition metal ions other than Pb 2+ ions was added [12].…”

Section: Resultsmentioning

confidence: 80%

“…In this case, it could be due to the Pb(II)-dithizone, since the peaks increase along with an increase in Pb(II)-dithizone concentration. The colloid of AuNPs can be stabilized by dithizone, which has the absorbance peak at 470 nm [27].…”

Section: Resultsmentioning

confidence: 99%

Colorimetric Sensing of Pb2+ Ion by Using Ag Nanoparticles in the Presence of Dithizone

et al. 2019

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Colorimetric analysis of heavy metal ions can be realized by the aid of Ag nanoparticles to improve the analytical characteristics. The method is based on the localized surface plasmon resonance (LSPR) properties of the Ag nanoparticles (AgNPs). In this work, we applied the AgNPs with the addition of dithizone to further improve the selectivity and sensitivity of Pb2+ analysis. Colorimetric sensing of Pb2+ ions based on the polyvinyl alcohol (PVA)-stabilized-colloidal AgNPs in the presence of dithizone is reported. A linear decrease in the AgNPs LSPR absorbance at 421 nm was observed along with the increase in the Pb2+ concentration in the range of 0.50–10 µg/L. The other ions give a minor change in the LSPR absorbance of colloidal AgNPs. The Pb2+ limit of detection, the limit of quantification, and sensitivity were found to be 0.64 ± 0.04 µg/L, 2.1 ± 0.15 µg/L, 0.0282 ± 0.0040 L/µg (n = 5), respectively. The obtained sensitivity is comparable with that of the immunosensing method. The proposed method could offer a good alternative for colorimetric analysis of Pb2+ ions by using nanoparticles in the presence of ligands, which can improve selectivity.

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“…1 ). GSH molecules are believed to form certain intermolecular hydrogen binding among themselves, even when they are adsorbed on AuNPs 24 . The concentration ratio of GSH and AuNP, thus, is one of the important factors that affects the aggregation rate in the solution.…”

Section: Resultsmentioning

confidence: 99%

Fabrication and Verification of Conjugated AuNP-Antibody Nanoprobe for Sensitivity Improvement in Electrochemical Biosensors

Khashayar

Amoabediny

Larijani

et al. 2017

Sci Rep

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This study was designed to obtain covalently coupled conjugates as means for achieving higher stability and better coverage of the AuNPs by antibodies on the particle surface suitable for sensor performance enhancement. Starting by using a modified protocol, colloid gold solution, with mean AuNP core size of ~6 nm was synthesized. The protocol used for conjugation of AuNPs to osteocalcin antibody in this study relies on covalent and electrostatic attractions between constituents. Varieties of conjugates with varying combinations of crosslinkers and different concentrations were successfully synthesized. The obtained products were characterized and their properties were studied to determine the best candidate in sense of antibody - antigen reactivity. Using AuNP-GSH-NHS-Ab combination (1:1:1), the tertiary structure of the protein was maintained and thus the antibody remained functional in the future steps. This one-pot method provided a simple method for covalently coupling antibodies on the particle surface while keeping their functionality intact. The AuNP content of the solution also accelerated electron transfer rate and thus amplifies the detection signal. With the developed and discussed technique herein, a simple solution is modeled to be used for measuring serum levels of biomarkers in single and/or multiplexed sensor systems.

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A Fast Colourimetric Assay for Lead Detection Using Label-Free Gold Nanoparticles (AuNPs)

Cited by 28 publications

References 37 publications

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Colorimetric Sensing of Pb2+ Ion by Using Ag Nanoparticles in the Presence of Dithizone

Fabrication and Verification of Conjugated AuNP-Antibody Nanoprobe for Sensitivity Improvement in Electrochemical Biosensors

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