A fast ethanol assay to detect seed deterioration

Kodde, Jan; Buckley, W. T.; Groot, Corine C. de; Retiere, Marie; Zamora, Ana Marcela Viquez; Groot, S.P.C.

doi:10.1017/s0960258511000274

Cited by 35 publications

(45 citation statements)

References 28 publications

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“…Mendes et al (2009) and Aumonde et al (2012) On the other hand, Crispin et al (1994), Buckley and Huang (2011) and Kodde et al (2012) reported the opposite trend in seeds of G. max, B. napus and Brassica oleracea L. respectively with the use of titration or by gas chromatography. The difference in results may result from incubation temperature.…”

Section: Lotsmentioning

confidence: 99%

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Vigor of canola seeds through quantification of CO2 emission

et al. 2013

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Seed marks the beginning of grain production from cultivated crops. Ensuring seed quality is the priority in the field of seed technology. In this context, the purpose of this study was to evaluate the use of carbon dioxide concentration to classify Brassica napus seed lots of different quality resulting from various sowing dates. Seed lots were evaluated by percentage of normal plantlets and the germination rate index. CO 2 concentration was quantified in samples submitted to five periods of incubation at 40° C with the aid of a gas exchange analyzer coupled to an injection and airflow system. The results were subjected to analysis of variance and the mean values were compared by the Duncan test and by regression analysis at the 5% level of significance. B. napus seeds from all lots showed a high percentage of normal plantlets and germination rate index. Percentage of normal plantlets was correlated with CO 2 concentration. Determination of the CO 2 concentration was more efficient with three hours of incubation which allowed classification of canola seed lots into four vigor classes. CO 2 concentration was inversely proportional to the percentage of normal plantlets.Index terms: Brassica napus L., seed respiration, infrared gas analyser, physiological quality. RESUMOA semente marca o inicio da produção de grãos de plantas cultivadas. Assegurar a qualidade da semente é a prioridade da tecnologia de sementes. Neste contexto, o trabalho objetivou aferir o uso da concentração de dióxido de carbono na classificação de sementes de Brassica napus de diferentes níveis de qualidade resultante de diferentes datas de semeadura. nos quais avaliou-se a porcentagem de plântulas normais e o índice de velocidade de germinação. A concentração CO 2 foi quantificada em amostras mantidas por cinco períodos de incubação a 40 °C e mensuradas com o auxílio de um medidor de trocas gasosas, acoplado a um sistema de injeção e fluxo de ar. Os resultados foram submetidos à análise de variância e as médias comparadas pelo teste de Duncan e pela análise de regressão a 5%. Os resultados da porcentagem de plântulas normais foram correlacionados com os resultados da concentração de CO 2 . Sementes de B. napus apresentaram alta porcentagem de plântulas normais. Contudo, para o índice de velocidade de germinação houve possibilidade de classificação em pelo menos duas classes de vigor. A determinação da concentração de CO 2 foi mais eficiente após três horas de incubação, sendo possível agrupar os lotes em quatro classes de vigor. A concentração de CO 2 foi inversamente proporcional à porcentagem de plântulas normais, com melhor coeficiente de correlação obtido após três horas de incubação.Termos para indexação: Brassica napus L., respiração de sementes, analisador infravermelho de gases, qualidade fisiológica.

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Section: Lotsmentioning

confidence: 99%

“…Seed performance in the field is a complex characteristic related to the environmental, physiological and genetic aspects of seeds which cannot be measured in a single location or with few replications (MARCOS-FILHO, 2005;KODDE et al, 2012).…”

Section: Lotsmentioning

confidence: 99%

Vigor of canola seeds through quantification of CO2 emission

et al. 2013

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“…(CGR): 100 × GR/FGP, where FGP corresponds to the final GP (Dewir et al 2011) and T 50 : number of days at which the seeds attained 50 % of their final GP (Kodde et al 2012).…”

Section: The Test Duration Was 60 Daysmentioning

confidence: 99%

Seed germination in <em>Ormosia macrocalyx</em>, an endangered tropical forest tree

et al. 2017

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Background: Ormosia macrocalyx is a tropical forest tree classified as endangered. Its seeds experience problems of dispersion and apparent physical dormancy due to their hard seed coating. Hypotheses: 1) The stages of dehiscence of the fruits of Ormosia macrocalyx influence the germinative behavior of its seeds. 2) Pregerminative treatments will improve the germination process of the seeds stored under refrigeration. Study species: Ormosia macrocalyx Study site and period: Villahermosa, Tabasco, Mexico from October 2012 to October 2014 Methods: Two completely randomized experiments were conducted in order to analyze the germination process by 1) evaluating germination in seeds from fruit at three stages of dehiscence (closed, semi-open, and open fruit) and 2) applying four treatments, including three pregerminative treatments (water soaking for 24 h, mechanical scarification and scarification+1% gibberellic acid) plus an untreated control, to seeds stored under refrigeration for 17 months. Results: Differences were found in germination rate (GR), corrected germination rate (CGR) and time to attain 50 % germination (T 50 ) among treatments in the first experiment, with the seeds from open fruits presenting the lowest response (3.31 % day -1 for GR and CGR, and 15.8 days for T 50 ), although all treatments showed similar times for the initiation of germination (GI) and final germination percentage (GP). In the second experiment, the effect of scarification was greater than both the control and soaking treatments. Scarification treatment values were 68.0 % (GP), 12.0 days (GI), 4.53 % day -1 (GR), 6.65 (CGR) and 14.5 days (T 50 ). Addition of gibberellic acid did not produce any further advantage over scarification alone. Conclusions:Seeds from open fruits have lower germination rates, although the final GP is not affected. Stored seeds present physical dormancy but lose viability with storage and, when collected after remaining attached to the tree for undefined periods, scarification can greatly improve their germination rate. Key words: fruit maturation, dormancy, pregerminative treatments, storage Germinación de semillas de Ormosia macrocalyx, un árbol forestal amenazado ResumenAntecedentes: Ormosia macrocalyx es un árbol tropical amenazado. Sus semillas tienen problemas de dispersión y aparente latencia física por cubierta seminal dura. Hipótesis: 1) Las etapas de dehiscencia de los frutos de Ormosia macrocalyx repercutirán en el comportamiento germinativo de sus semillas. 2) Los tratamientos pregerminativos mejorarán el proceso germinativo de las semillas almacenadas en refrigeración Especie de estudio: Ormosia macrocalyx Ducke. Sitio de estudio y fechas: Villahermosa, Tabasco, México, octubre 2012-octubre 2014 Métodos: Se establecieron dos experimentos completamente aleatorios para analizar el proceso de la germinación: 1) Evaluación de la germinación en semillas de frutos en tres estados de madurez (cerrados, semi-abiertos y abiertos), y 2) Aplicación de cuatro tratamientos, tres pregerminativos (r...

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“…However, deterioration occurs in the process of seeds storage, which causes a drop of seed vigor, poor seedling emergence and bad influence on intracellular physiological reactions (McDonald, 1999;Kapoor et al, 2011). Although the exact mechanisms of seed vigour losing are still under research, the lipid peroxidation has been considered as the main explanation for seed deterioration due to accumulation of reactive oxygen species (ROS) (Bailly et al, 2008;Kodde et al, 2012). Furthermore, deterioration is related to loss of membrane integrity, cell structural disruptions and other various cellular changes such as DNA degradation, protein denaturation and ultimately causes cell death (McDonald, 1999;Karuppanapandian et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Impacts of Ascorbic Acid on Germination, Antioxidant Enzymes and Ultrastructure of Embryo Cells of Aged Elymus sibiricus Seeds with Different Moisture Contents

HuiFang¹,

Mao²,

Sun³

et al. 2015

IJAB

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This study determined the effects of exogenous ascorbic acid (AsA) treatment on germination, membrane lipid peroxidation, activities of antioxidant enzymes and ultra-structure of embryo cells of Siberian wildrye (Elymus sibiricus L.) seeds with different moisture contents (4%, 10% and 16%) after ageing at 45°C for 48 h. Germination percentage and activities of enzymes declined, electrical conductivity value of seeds increased significantly as moisture content increased from 4% to 16%. However, ascorbic acid treatment had protective effects on alleviating the damage of ageing to seeds membrane integrity and structure of mitochondria, which was in accordance with the decrease of electrical conductivity and malondialdehyde (MDA) content, especially for seeds with 10% moisture content. Ascorbic acid treatment significantly (P<0.05) inhibited the loss of seed germination at 10% moisture content, and correspondingly electrical conductivity decreased and activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) improved. In addition, ultra structure observation of embryo cells showed that serious damage occurred in seeds with 16% moisture content, including the broken cellular and nuclear membrane, pyknotic nucleolus and swollen mitochondria. Although activities of CAT, APX and GR were improved by the exogenous AsA, it had no obviously protective effect on integrity of membrane system and mitochondria. Seed moisture of Siberian wildrye played a vital role in the function of AsA, which provided an efficient treatment to deal with the antioxidant effects of storage and ageing at seed moisture of 10%.

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A fast ethanol assay to detect seed deterioration

Cited by 35 publications

References 28 publications

Vigor of canola seeds through quantification of CO2 emission

Vigor of canola seeds through quantification of CO2 emission

Seed germination in <em>Ormosia macrocalyx</em>, an endangered tropical forest tree

Impacts of Ascorbic Acid on Germination, Antioxidant Enzymes and Ultrastructure of Embryo Cells of Aged Elymus sibiricus Seeds with Different Moisture Contents

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