A fatal affair: Circulating tumor cell relationships that shape metastasis

Ozimski, Lauren L; Gremmelspacher, David; Aceto, Nicola

doi:10.1016/j.isci.2021.103073

Cited by 9 publications

(3 citation statements)

References 93 publications

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“…Single-cell DNA methylation sequencing has been widely used to profile rare cells and investigate cellular heterogeneity. CTCs preserve tumor genetic and epigenetic information well and are excellent candidates for cancer prognosis and diagnosis [138][139][140]. It is foreseeable that single-cell methylation sequencing and site-specific methylation assays will be incorporated into clinical testing.…”

Section: Perspectivesmentioning

confidence: 99%

Technologies and applications of single-cell DNA methylation sequencing

Liu¹,

Wang²,

Gu³

et al. 2023

Theranostics

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DNA methylation is the most stable epigenetic modification. In mammals, it usually occurs at the cytosine of CpG dinucleotides. DNA methylation is essential for many physiological and pathological processes. Aberrant DNA methylation has been observed in human diseases, particularly cancer. Notably, conventional DNA methylation profiling technologies require a large amount of DNA, often from a heterogeneous cell population, and provide an average methylation level of many cells. It is often not realistic to collect sufficient numbers of cells, such as rare cells and circulating tumor cells in peripheral blood, for bulk sequencing assays. It is therefore essential to develop sequencing technologies that can accurately profile DNA methylation using small numbers of cells or even single cells. Excitingly, many single-cell DNA methylation sequencing and single-cell omics sequencing technologies have been developed, and applications of these methods have greatly expanded our understanding of the molecular mechanism of DNA methylation. Here, we summaries single-cell DNA methylation and multi-omics sequencing methods, delineate their applications in biomedical sciences, discuss technical challenges, and present our perspective on future research directions.

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Section: Perspectivesmentioning

confidence: 99%

Technologies and applications of single-cell DNA methylation sequencing

Liu¹,

Wang²,

Gu³

et al. 2023

Theranostics

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“…Cluster formation is produced in part by contact between a cell-cell ligand-protein or cytokine receptor and another cell [74]. An aggregate of more than one cancer cell is a homotypic CTC cluster; in combination with other cell types, such as immune cells or stromal cells, are heterotypic CTC clusters [75]. Compared to the single cells present in the circulation, these CTC clusters have an intercellular adhesion component that can promote tumor metastasis [76].…”

Section: Introductionmentioning

confidence: 99%

The role of extracellular vesicles in circulating tumor cell-mediated distant metastasis

Guo,

Huang,

et al. 2023

Mol Cancer

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Current research has demonstrated that extracellular vesicles (EVs) and circulating tumor cells (CTCs) are very closely related in the process of distant tumor metastasis. Primary tumors are shed and released into the bloodstream to form CTCs that are referred to as seeds to colonize and grow in soil-like distant target organs, while EVs of tumor and nontumor origin act as fertilizers in the process of tumor metastasis. There is no previous text that provides a comprehensive review of the role of EVs on CTCs during tumor metastasis. In this paper, we reviewed the mechanisms of EVs on CTCs during tumor metastasis, including the ability of EVs to enhance the shedding of CTCs, protect CTCs in circulation and determine the direction of CTC metastasis, thus affecting the distant metastasis of tumors.

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“…A versatile and facile strategy for evaluating drug sensitivity of cancer patients is highly needed.Circulating tumor cells (CTCs) shed from solid tumor lesions and circulate in the bloodstream, and they contain the whole information relating to their origin. [9][10][11] Currently, CTCs can be enriched from vein blood non-invasively by various strategies [12][13][14] and are expected to reflect drug sensitivity of the tumors. [15,16] A pharmacogenomic profiling technology was developed to reveal gene expression of CTCs to identify pancreatic cancer patients who were sensitive to chemotherapy regimens.…”

mentioning

confidence: 99%

Gelatin‐Coated ZnO Array Nanoplatform for Capture, Drug Resistance Identification, and Recovery of Circulating Tumor Cells

Dai

Cai

Zhang

et al. 2023

Adv Materials Technologies

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Drug resistance causes failure of cancer treatment. Identifying drug sensitivity of tumors can guide therapeutic administration for cancer patients, however this approach lacks a facile, fast, and noninvasive evaluation platform. Herein, a gelatin‐coated ZnO array nanoplatform to isolate circulating tumor cells (CTCs) is developed, and their drug resistance for inspecting tumor sensitivity is assessed. The nanoplatform is comprised of a nanosubstrate of ZnO submicropillars (ZnO SMPs) for efficiently isolating cancer cells, and Au nanoparticles (AuNPs) based electrochemical probes for evaluating drug resistance of the captured cells. Gelatin, an extracellular matrix‐like and enzyme‐degradable material, is used to coat ZnO SMPs and AuNPs, endowing the nanoplatform with the ability to maintain cancer cell viability and realize enzyme‐responsive cell recovery for downstream bioanalysis. This nanoplatform is expected to noninvasively evaluate drug sensitivity of cancer patients through simple liquid biopsy of CTCs in peripheral blood and benefit the treatment.

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A fatal affair: Circulating tumor cell relationships that shape metastasis

Cited by 9 publications

References 93 publications

Technologies and applications of single-cell DNA methylation sequencing

Technologies and applications of single-cell DNA methylation sequencing

The role of extracellular vesicles in circulating tumor cell-mediated distant metastasis

Gelatin‐Coated ZnO Array Nanoplatform for Capture, Drug Resistance Identification, and Recovery of Circulating Tumor Cells

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