A feasibility study of chemosensitivity assay by adhesive tumor cell culture system using biopsy specimens for gastric cancer

Suzuki, Takao; Ochiai, Takenori; Nakajima, Kiyokazu; Matsubara, Hisahiro; Arima, Miwako; Hori, Seiji; Hayashi, Hideki; Shimada, Hideaki; Takeda, Akihiko; Yanagawa, Tetsuo; Sen, Katsunei

doi:10.1002/1096-9098(200006)74:2<141::aid-jso12>3.0.co;2-x

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Based on the overall results, GC cells were more susceptible to CDDP and 5-FU than to ADM and MMC, which coincided with previous reports [24,25]. TAX is a new drug used in chemotherapy for GC [26,27], to which GC cells were the most susceptible, and should be widely used in GC.…”

Section: Discussionsupporting

confidence: 88%

Correlation between chemosensitivity to anticancer drugs and telomerase reverse transcriptase mRNA expression in gastric cancer

Wang

Geng

et al. 2013

Diagn Pathol

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BackgroundThe determination of sensitive chemotherapy drugs for gastric cancer (GC) is one of the greatest challenges of adjuvant therapy. Here we evaluated the chemosensitivity of GC to anticancer drugs and the telomerase reverse transcriptase (hTERT) mRNA expression, and investigated the relationship of them.MethodsThe GC cells which were collected from 68 patients with primary GC were primary cultured. The chemosensitivity of GC cells to anticancer drugs was evaluated successfully using the MTT assay for 60 cases of GC cells, and the hTERT mRNA expression was examined in 60 cases of GC tissues and corresponding normal gastric mucosa and 6 cases of chronic superficial gastritis mucosa by in situ hybridization.ResultsTaxol, Cisplatin and 5-Fluorouracil were in general more effective than Adriamycin and Mitomycin for GC cells, and the chemosensitivity to anticancer drugs was associated with tumor histological types and a worse tumor grade. Compared to normal gastric mucosa tissues, hTERT mRNA expression was significantly increased in GC (P<0.05), which was related with a worse differentiation and drug-resistance to 5-Fluorouracil or Adriamycin in GC.ConclusionsThese data demonstrate for the first time that examinations of hTERT mRNA expression as an important factor could be used to select the chemotherapeutic drugs for GC patients.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1793217009875483

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Section: Discussionsupporting

confidence: 88%

Correlation between chemosensitivity to anticancer drugs and telomerase reverse transcriptase mRNA expression in gastric cancer

Wang

Geng

et al. 2013

Diagn Pathol

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Drug sensitivity testing platforms for gastric cancer diagnostics

Lau¹,

Wong

et al. 2015

J Clin Pathol

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Gastric cancer diagnostics has traditionally been histomorphological and primarily the domain of surgical pathologists. Although there is an increasing usage of molecular and genomic techniques for clinical diagnostics, there is an emerging field of personalised drug sensitivity testing. In this review, we describe the various personalised drug sensitivity testing platforms and discuss the challenges facing clinical adoption of these assays for gastric cancer.

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A feasibility study of chemosensitivity assay by adhesive tumor cell culture system using biopsy specimens for gastric cancer

Abstract: Although lacking in statistical significance, the results suggest that the drug to which the tumor revealed sensitivity in the ATCCS would produce histological effects and the drug to which the tumor was resistant would have no histological effect.

Cited by 2 publications

References 19 publications

Correlation between chemosensitivity to anticancer drugs and telomerase reverse transcriptase mRNA expression in gastric cancer

Correlation between chemosensitivity to anticancer drugs and telomerase reverse transcriptase mRNA expression in gastric cancer

Drug sensitivity testing platforms for gastric cancer diagnostics

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