2003
DOI: 10.1016/s0014-5793(03)00559-3
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A ferredoxin Arg‐Glu pair important for efficient electron transfer between ferredoxin and ferredoxin‐NADP+ reductase

Abstract: In order to elucidate the importance of a ferredoxin (Fd) Arg-Glu pair involved in dynamic exchange from intra-to intermolecular salt bridges upon complex formation with ferredoxin-NADP + oxidoreductase (FNR), Equisetum arvense FdI and FdII were investigated as normal and the pair-lacking Fd, respectively. The FdI mutant lacking this pair was unstable and rapidly lost the [2Fe^2S] cluster. The catalytic constant (k cat ) of the electron transfer for FdI is 5.5 times that for FdII and the introduction of this p… Show more

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Cited by 11 publications
(8 citation statements)
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“…K m values of both FdI and FdII were ϳ0.6 M, and this similarity is in contrast to our previous measurements of electron transfer in the opposite direction (from FNR to Fd) where the K m value of FdI was around 7 times greater than that of FdII (13). Consistent with our previous measurements, V max for electron transfer with FNR was greater with FdI than FdII, although it was 1.3 times greater in electron transfer from Fd to FNR, as compared with 5.5 times greater in the opposite direction (13).…”
Section: Quality Of E Arvense Fdiicontrasting
confidence: 99%
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“…K m values of both FdI and FdII were ϳ0.6 M, and this similarity is in contrast to our previous measurements of electron transfer in the opposite direction (from FNR to Fd) where the K m value of FdI was around 7 times greater than that of FdII (13). Consistent with our previous measurements, V max for electron transfer with FNR was greater with FdI than FdII, although it was 1.3 times greater in electron transfer from Fd to FNR, as compared with 5.5 times greater in the opposite direction (13).…”
Section: Quality Of E Arvense Fdiicontrasting
confidence: 99%
“…Preparation of Recombinant Fds-E. arvense recombinant FdII, FdI, and the FdII mutants were expressed and accumulated as the apo-form in Escherichia coli cells and successfully converted to the holo-form by chemical reconstitution of the [2Fe-2S] cluster as described previously (13). Further purification of the Fds was carried out as described previously (15,16).…”
Section: Methodsmentioning
confidence: 99%
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