A first-generation porcine whole-genome radiation hybrid map

Hawken, Rachel; Murtaugh, J.; Flickinger, Gail H.; Yerle, Martine M.; Robic, Annie; Milan, Denis; Gellin, Joël; Beattie, Craig W.; Schook, Lawrence B.; Alexander, L. J.

doi:10.1007/s003359901097

Cited by 249 publications

(219 citation statements)

References 45 publications

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“…These clones contained genes or microsatellite markers previously located on the porcine cytogenetic (http://www.toulouse.inra.fr/lgc/pig/ cyto/cyto.htm) and RH maps [18] (Tab. I).…”

Section: Preparation Of the Probesmentioning

confidence: 99%

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

2004

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-Many chromosomal rearrangements are detected each year in France on young boars candidates for reproduction. The possible use of these animals requires a good knowledge of the potential effect of the rearrangements on the prolificacy of their mates. This effect can be estimated by an accurate determination of the rate of unbalanced spermatozoa in the semen of boars which carry the rearrangements. Indeed, these spermatozoa exhibiting normal fertilizing ability are responsible for an early embryonic mortality, and then, for a decrease of the litter sizes. The "spermFISH" technique, i.e. fluorescent in situ hybridization on decondensed sperm heads, has been used on several occasions in Man, in this perspective. In livestock species, this method was formerly used mainly for semen sexing purposes. We used it, for the first time, to estimate the rates of imbalance in the semen of four boars carrying chromosomal rearrangements: two reciprocal translocations, rcp(3;15)(q27;q13) and rcp(12;14)(q13;q21), as well as two independent cases of trisomy 18 mosaicism. The rates of unbalanced gametes were relatively high for the two reciprocal translocations (47.83% and 24.33%, respectively). These values differed from the apparent effects of the rearrangements estimated using a limited number of litters: a decrease in prolificacy of 23% (estimation obtained using the results of 6 litters) and 39% (57 litters), respectively for the 3/15 and 12/14 translocations. The imbalance rates were much lower for the trisomy mosaics (0.58% and 1.13%), suggesting a very moderate effect of this special kind of chromosomal rearrangement.

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“…These clones contained genes or microsatellite markers previously located on the porcine cytogenetic (http://www.toulouse.inra.fr/lgc/pig/ cyto/cyto.htm) and RH maps [18] (Tab. I).…”

Section: Preparation Of the Probesmentioning

confidence: 99%

Estimation of the proportion of genetically unbalanced spermatozoa in the semen of boars carrying chromosomal rearrangements using FISH on sperm nuclei

2004

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“…An advantage of RH mapping is that different doses of radiation can be used to construct maps with varying levels of resolution Lunetta et al 1996;Schuler et al 1996;Stewart et al 1997). This work has been followed by RH mapping in a number of other animal species (McCarthy et al 1997;Priat et al 1998;Yerle et al 1998;Hawken et al 1999;Hukriede et al 1999;Kwok et al 1999;Murphy et al 1999;Vignaux et al 1999;Watanabe et al 1999). In addition to whole-genome RH mapping, other researchers have focused on mapping specific chromosomes and chromosomal regions in different species as well (Hoover and Floros 1998;Barendse et al 2000;Ozawa et al 2000;Liu et al 2002;Rink et al 2002).…”

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confidence: 99%

High-Resolution Radiation Hybrid Map of Wheat Chromosome 1D

Kalavacharla

Hossain

et al. 2006

Genetics

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Physical mapping methods that do not rely on meiotic recombination are necessary for complex polyploid genomes such as wheat (Triticum aestivum L.). This need is due to the uneven distribution of recombination and significant variation in genetic to physical distance ratios. One method that has proven valuable in a number of nonplant and plant systems is radiation hybrid (RH) mapping. This work presents, for the first time, a high-resolution radiation hybrid map of wheat chromosome 1D (D genome) in a tetraploid durum wheat (T. turgidum L., AB genomes) background. An RH panel of 87 lines was used to map 378 molecular markers, which detected 2312 chromosome breaks. The total map distance ranged from $3,341 cR 35,000 for five major linkage groups to 11,773 cR 35,000 for a comprehensive map. The mapping resolution was estimated to be $199 kb/break and provided the starting point for BAC contig alignment. To date, this is the highest resolution that has been obtained by plant RH mapping and serves as a first step for the development of RH resources in wheat.

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“…Six groups of fibres (pure: slow, 2A, 2X and 2B, and hybrid: 2A-2X and 2X-gene expresssion in the pig is quickly growing (Hawken et al, 1999;Yao et al, 2002) and makes the pig a candidate for complete genome sequencing.…”

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confidence: 99%