A First Step in the Quest for the Active Constituents in Filipendula ulmaria (Meadowsweet): Comprehensive Phytochemical Identification by Liquid Chromatography Coupled to Quadrupole-Orbitrap Mass Spectrometry

Bijttebier, Sebastiaan; Auwera, Anastasia Van der; Voorspoels, Stefan; Noten, Bart; Hermans, Nina; Pieters, Luc; Apers, Sandra

doi:10.1055/s-0042-101943

Cited by 45 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One solution is the use of pseudo-MS 3 spectra, which is obtained from a combination of in-source fragmentation with high cone voltage and subsequent CID or HCD fragmentation in conventional mass spectrometer 23 . The aglycone moieties of flavonoids were identified by comparing pseudo-MS 3 spectra with the MS 2 spectra of the authentic standards of the aglycones 3, 24–26 . Therefore, it will become possible to apply FlavonoidSearch to pseudo-MS 3 spectra.…”

Section: Discussionmentioning

confidence: 99%

FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry

Akimoto

Ara

Nakajima

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Currently, in mass spectrometry-based metabolomics, limited reference mass spectra are available for flavonoid identification. In the present study, a database of probable mass fragments for 6,867 known flavonoids (FsDatabase) was manually constructed based on new structure- and fragmentation-related rules using new heuristics to overcome flavonoid complexity. We developed the FlavonoidSearch system for flavonoid annotation, which consists of the FsDatabase and a computational tool (FsTool) to automatically search the FsDatabase using the mass spectra of metabolite peaks as queries. This system showed the highest identification accuracy for the flavonoid aglycone when compared to existing tools and revealed accurate discrimination between the flavonoid aglycone and other compounds. Sixteen new flavonoids were found from parsley, and the diversity of the flavonoid aglycone among different fruits and vegetables was investigated.

show abstract

Section: Discussionmentioning

confidence: 99%

FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry

Akimoto

Ara

Nakajima

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The HPLC-DAD-QTOF analysis of the Coccoloba cowellii extract was carried out following the standard protocols from the Natural Products and Food Research and Analysis (NatuRA) group [46,47]. The mobile phase consisted of H 2 O + 0.1% FA (A) and ACN + 0.1% FA (B), and the gradient was set as follows (min/B%): 0.0/2.0, 1.0/2.0, 14.0/26.0, 24.0/65.0, 26.0/100.0, 29.0/100.0, 31.0/2.0, 36.0/2.0.…”

Section: Uhplc-hrms Analysismentioning

confidence: 99%

Chemical and Pharmacological Potential of Coccoloba cowellii, an Endemic Endangered Plant from Cuba

et al. 2021

Self Cite

View full text Add to dashboard Cite

Coccoloba cowellii Britton (Polygonaceae) is an endemic and critically endangered plant that only grows in Camagüey, a province of Cuba. In this study, a total of 13 compounds were identified in a methanolic leaf extract, employing a dereplication of the UHPLC-HRMS data by means of feature-based molecular networking (FBMN) analysis in the Global Natural Products Social Molecular Network (GNPS), together with the interpretation of the MS/MS data and comparison with the literature. The major constituents were glucuronides and glycosides of myricetin and quercetin, as well as epichatechin-3-O-gallate, catechin, epicatechin and gallic acid, all of them being reported for the first time in C. cowellii leaves. The leaf extract was also tested against various microorganisms, and it showed a strong antifungal effect against Candida albicans ATCC B59630 (azole-resistant) (IC50 2.1 µg/mL) and Cryptococcus neoformans ATCC B66663 (IC50 4.1 µg/mL) with no cytotoxicity (CC50 > 64.0 µg/mL) on MRC-5 SV2 cells, determined by the resazurin assay. Additionally, the extract strongly inhibited COX-1 and COX-2 enzyme activity using a cell-free experiment in a dose-dependent manner, being significantly more active on COX-1 (IC50 4.9 µg/mL) than on COX-2 (IC50 10.4 µg/mL). The constituents identified as well as the pharmacological activities measured highlight the potential of C. cowellii leaves, increasing the interest in the implementation of conservation strategies for this species.

show abstract

“…and F. vulgaris Moench, which are officinal plant species in many countries. Researchers have found in these species derivatives of salicylic acid [ 9 , 10 ], 2-pyrone-4,6-dicarboxylic acid [ 11 , 12 ], flavonoids [ 13 , 14 ], tannins [ 15 , 16 ] and essential oils [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Meadowsweet Teas as New Functional Beverages: Comparative Analysis of Nutrients, Phytochemicals and Biological Effects of Four Filipendula Species

2016

View full text Add to dashboard Cite

In recent years, the increased popularity of functional beverages such as herbal teas and decoctions has led to the search for new sources of raw materials that provide appropriate taste and functionality to consumers. The objective of this study was to investigate the nutritional, phytochemical profiles and bioactivities of possible functional beverages produced from F. ulmaria and its alternative substitutes (F. camtschatica, F. denudata, F. stepposa). The investigated decoctions were analyzed regarding their macronutrient, carbohydrate, organic acid, amino acid and mineral composition. Quantification of the main phenolic compounds in the decoctions of meadowsweet floral teas was performed by a microcolumn RP-HPLC-UV procedure; the highest content was revealed in F. stepposa tea. The investigation of the essential oil of four meadowsweet teas revealed the presence of 28 compounds, including simple phenols, monoterpenes, sesquiterpenes and aliphatic components. The dominance of methyl salicylate and salicylaldehyde was noted in all samples. Studies on the water soluble polysaccharides of Filipendula flowers allowed us to establish their general affiliation to galactans and/or arabinogalactans with an admixture of glucans of the starch type and galacturonans as minor components. The bioactivity data demonstrated a good ability of meadowsweet teas to inhibit amylase, α-glucosidase and AGE formation. Tea samples showed antioxidant properties by the DPPH•, ABTS•+ and Br• free radicals scavenging assays and the carotene bleaching assay, caused by the presence of highly active ellagitannins. The anti-complement activity of the water-soluble polysaccharide fraction of meadowsweet teas indicated their possible immune-modulating properties. Filipendula beverage formulations can be expected to deliver beneficial effects due to their unique nutritional and phytochemical profiles. Potential applications as health-promoting functional products may be suggested.

show abstract

A First Step in the Quest for the Active Constituents in Filipendula ulmaria (Meadowsweet): Comprehensive Phytochemical Identification by Liquid Chromatography Coupled to Quadrupole-Orbitrap Mass Spectrometry

Cited by 45 publications

References 34 publications

FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry

FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry

Chemical and Pharmacological Potential of Coccoloba cowellii, an Endemic Endangered Plant from Cuba

Meadowsweet Teas as New Functional Beverages: Comparative Analysis of Nutrients, Phytochemicals and Biological Effects of Four Filipendula Species

Contact Info

Product

Resources

About