A fixed allele at microsatellite locus LS-39 exhibiting species-specificity for the black caviar producer Acipenser stellatus

Jenneckens, Ingo; Meyer, J. N.; Hörstgen‐Schwark, Gabriele; May, Bernie; Debus, L.; Wedekind, Helmut; Ludwig, Arne

doi:10.1046/j.1439-0426.2001.00234.x

Cited by 43 publications

(22 citation statements)

References 20 publications

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“…We considered individuals with three to four alleles per locus to be polyploid. A number of individuals and genotypes only had diploid microsatellite phenotypes (i.e., a maximum of two alleles per locus) and were subjected to an allele dosage analysis (Jenneckens et al 2001) to indicate whether they were diploid or polyploid. The basic assumption behind an allele dosage approach is that within a single PCR, alleles of the same locus are amplified quantitatively in relation to the copy number in which they occur in the nucleus of each cell, for example, when only two alleles are present (say, alleles A and B).…”

Section: Methodsmentioning

confidence: 99%

Asexuality and polyploidy in Daphnia from the tropical Andes

Aguilera

Mergeay

Wollebrants

et al. 2007

Limnology & Oceanography

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We assessed genetic variation at microsatellite loci within and among populations of the planktonic crustacean Daphnia pulex in 12 Bolivian Andean lakes, located above 4,000 m above sea level. Genetic analyses show that all populations consisted of obligately asexual lineages, a fact that was confirmed by observations from laboratory cultures. Moreover, microsatellite phenotypes indicate that these tropical lineages are polyploid. Levels of genetic diversity were comparable to those found in polyploid Daphnia from arctic regions, indicating a local origin rather than an accidental colonization from arctic regions. This is the first record of polyploid cladocerans in a tropical region. We suggest that their origination and abundance have probably been facilitated by the extreme environmental conditions in Andean lakes. Our analysis of multilocus genotype frequencies in relation to variation in environmental conditions indicates lineage sorting along a food availability and fish predation gradient.

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Section: Methodsmentioning

confidence: 99%

Asexuality and polyploidy in Daphnia from the tropical Andes

Aguilera

Mergeay

Wollebrants

et al. 2007

Limnology & Oceanography

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“…The number of SNPs required for correct assignment of market place fish to full sib families on a single farm was up to 400, to multiplier individuals (parents) it was 74 and to nucleus individuals, was 200. SNPs have been used for species identification of food products as tuna (Kitaoka et al, 2008) and food product origin, including sturgeon caviar origin (Jenneckens et al, 2001;Ogden et al, 2013).…”

Section: Traceability Of Aquaculture Productionmentioning

confidence: 99%

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Wenne

2017

Aquat. Living Resour.

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-An increase in aquatic animal production can be achieved by extending aquaculture areas geographically, utilizing new species for culture, and using new technologies. Among new technologies useful for the increase of aquaculture production is the application of genetics and genomics. New molecular tools that benefit aquaculture have been developed. There has been a large number of experimental and review papers published concerning molecular markers and the range of their applications, including aquaculture and food product analyses. Analysis of single nucleotide polymorphisms (SNPs) has emerged as genotyping technology with wide and significant applications in aquaculture. SNPs can be used for construction of genetic linkage maps, finding quantitative trait loci (QTL) for useful traits like growth, body weight, grilsing, thermal and low oxygen tolerance, resistance to stress and diseases, mapping sex determination loci and identification of progeny in selection and chromosome manipulation experiments, assessment of genomic selectionand marker assisted selection in aquaculture. Genome-wide association studies (GWAS) facilitate the finding associations between SNPs and a trait in related or unrelated specimens. However, many traits are complex and can be controlled by number of QTL. Genotyping by genome reduction complexity sequencing emerged as an efficient and applicable technology in genomic selection. Identification of genes, sequences and nucleotides (substitutions) directly influencing phenotypic variations opens the possibility of marker-assisted selection for desirable characters in culture. SNP and QTL associations can be enhanced using genome editing technology. Examples of successful applications of SNPs in aquaculture of fish, crustacean and mollusk species, representing most geographic areas, and ecological risks assessment are reviewed.

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“…For instance, the genotype ABBC produces the microsatellite phenotype ABC, and cannot be reliably differentiated from genotypes AABC or ABCC. Jenneckens et al (2001) suggested to estimate the number of allele copies on the basis of peak heights and areas in the electropherograms as exemplified by the microsatellite locus LS-39 in the Siberian sturgeon Acipenser baerii. However, this approach seems not to be fully reliable: Congiu et al (2011) found that the four peaks of a complete heterozygous genotype did not always show the expected same height in Adriatic sturgeon, and the same discrepancy was observed in the present study of Russian sturgeon (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Validation of 12 species-specific, tetrasomic microsatellite loci from the Russian sturgeon, Acipenser gueldenstaedtii, for genetic broodstock management

et al. 2018

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The Russian sturgeon, Acipenser gueldenstaedtii, is a critically endangered fish species. Hatcheries are operated in several countries within its natural range to produce stocking material for release into the wild and also for aquaculture purposes (caviar and meat production). An appropriate genetic broodstock management (plan or strategy) is required to avoid negative effects, e.g., admixture and hybridization of genetically differing stocks or loss of genetic variability due to inbreeding and genetic drift. Therefore, 11 tetrasomic microsatellite loci were newly isolated from the Russian sturgeon genome and arranged together with an already known locus into four multiplex PCR sets. These microsatellites were used to characterize three groups of hatchery juveniles from Germany (aquaculture production), Turkey, and Romania (production of stocking material) as well as a group of wild-caught adults from the Danube River, Romania. Based on the variability within groups, measured by the mean number of alleles per locus and expected heterozygosity, and the differentiation between groups, measured by Nei's G ST and genetic distance D, the ability of the 12 loci to detect unwanted reductions in genetic variability within hatchery juveniles and to differentiate between groups could be demonstrated. This set of loci can also be used to identify those pairs of spawners that transmit the highest possible genetic variability to the next generation.

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A fixed allele at microsatellite locus LS-39 exhibiting species-specificity for the black caviar producer Acipenser stellatus

Cited by 43 publications

References 20 publications

Asexuality and polyploidy in Daphnia from the tropical Andes

Asexuality and polyploidy in Daphnia from the tropical Andes

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Validation of 12 species-specific, tetrasomic microsatellite loci from the Russian sturgeon, Acipenser gueldenstaedtii, for genetic broodstock management

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