A Flexible DNA Biosensor for Breast Cancer Marker BRCA1 Detection

Abstract

Early detection of breast cancer is of great significance to improve the prognosis and survival rate of breast cancer. However, how to detect breast cancer 1 gene mutation (BRCA1) efficiently in early stage is still a great challenge. In the past few decades, more and more attention has been paid to the design of a biosensor. In this work, A method for rapid detection of specific BRCA1 using silver nanowires modified polyethylene terephthalate (PET) film as a disposable electrode was proposed. With a high aspect ratio, AgNWs distributed in the PET film were easy to form a conductive network as a flexible electrode. A well‐conductive gold sputtering layer can prevent AgNW from falling off to provide a conductive and stable electrode substrate. The Au‐HRP can effectively reflect the hybridization matching phenomenon of target. Results indicated this strategy we proposed had a low detection limit with different concentrations of complementary DNA with limit of detection of 5.3×10−15 M in the range from 5.0×10−10 M to 5.0×10−7 M. Due to the high sensitivity, simplicity and cheap price, arbitrary cut, AgNWs/PET film becomes a promising electrode material for quantification of low concentration biological component in early diagnosis.

Singlet oxygen-based photoelectrochemical detection of DNA

“…Thus, the orientation of hybridized duplexes, their dynamics on the surface, and backfilling or contamination of the unoccupied surface affect the electrochemical response in a complex manner and may result in poorly reproducible data. For this reason, the use of enzymatic labels generating an electroactive product stays the most prominent analytical strategy (Liu et al 2020;Wang et al 2020).…”