A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction

Abstract

In this paper, a chemiluminescence (CL) method is proposed for retinol determination by combining flow injection (FI) methodology. The CL reaction is based on the oxidation of luminol by diperiodatoargentate(III) (DPA) in the presence of retinol. Under the optimum conditions, the relative CL intensity was linear to the concentration of retinol over the range 5.0310 23 -14 mg L 21 (y5347.26x12.5944, R 2 50.9999, n58) with limit of detection (LOD) of 1.5310 23 mg L 21 (S/N53) and limit of quantification (LOQ) of 5.0310 23 mg L 21 (S/N510). The relative standard deviation (RSD) was from 1.04-3.4% over the range studied and injection throughputs of 150 h 21 . The method was satisfactorily applied to retinol in pharmaceutical formulation samples. The samples were saponified and extracted with liquid-liquid extraction using ether as an extractant. The possible CL mechanism is supported by CL and UV-visible spectrophotometric studies.

Critical Review of Synthesis, Toxicology and Detection of Acyclovir

“…The schematic diagram of FI-CL is shown in Figure 7. Because of its advantages of high sensitivity, wide linear range, simple instruments and convenient operation, it is widely used in environmental monitoring, drug analysis, food analysis and so forth [101].…”

“…The schematic diagram of FI-CL is shown in Figure 7. Because of its advantages of high sensitivity, wide linear range, simple instruments and convenient operation, it is widely used in environmental monitoring, drug analysis, food analysis and so forth [101]. Using alkaline as medium, ACV can significantly inhibit Ni (IV) complexes-luminol LC systems, and there is a linear relationship between the inhibition extent and the concentration of ACV within a certain range.…”

Analysis of Vitamin A in Multivitamin Products

“…по 1], что позволило широко использовать его как в пищевых продуктах, так и в лекарственных препаратах. Для изучения метаболизма витамина А и его количественного определения используются различные химические и физико-химические методы анализа [2,3].…”

“…Колориметрическое/спектрофотометрическое определение [2,15,16] основано на измерении оптической плотности окрашенного комплекса, образующегося в результате дегидратации витамина А в присутствии кислот Льюиса при длине волны 620 нм и снижении поглощения при 325 нм при инактивации витамина А УФ-излучением. Для ряда лекарственных препаратов используются именно спектрофотометрические методики количественного определения эфиров ретинола и бетакаротена.…”