A Flowering Integrator, SOC1, Affects Stomatal Opening in Arabidopsis thaliana

Kimura, Yukitaka; Aoki, Saya; Ando, Eigo; Kitatsuji, Ayaka; Watanabe, Aiko; Ohnishi, Masato; Takahashi, Koji; Inoue, Shin‐ichiro; Nakamichi, Norihito; Tamada, Yosuke; Kinoshita, Toshinori

doi:10.1093/pcp/pcu214

Cited by 48 publications

(41 citation statements)

References 57 publications

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“…Constitutive open-stomata phenotypes have been reported for mutants overexpressing FT, TWIN SISTER OF FT, and SOC1 (Kimura et al, 2015) as well as for mutants overexpressing GI and CO, which are both known to be FT up-regulators. Conversely, mutations that repress FT cause stomatal closure (Kinoshita et al, 2011;Ando et al, 2013).…”

Section: Discussion the Guard Cell Circadian System May Be Fine-tunedmentioning

confidence: 99%

“…Intriguingly, several key genes in the circadian controlled pathway that regulates photoperiodic flowering have also been shown to affect stomatal aperture. In blue light, the floral integrator FLOWERING LOCUS T (FT) induces stomatal opening by enhancing the phosphorylation status of plasma membrane H + -ATPase (Kinoshita et al, 2011;Kimura et al, 2015;Ando et al, 2013), and GIGANTEA (GI), CONSTANS (CO), and ELF3 regulate FT levels; GI and CO up-regulate FT levels to cause stomatal opening, and ELF3 represses FT, resulting in stomatal closure (Kinoshita et al, 2011;Ando et al, 2013). However, the extent of to which the photoperiodic flowering and guard cell regulatory mechanisms are similar has not been explored.…”

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confidence: 99%

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CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

et al. 2017

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The endogenous circadian (;24 h) system allows plants to anticipate and adapt to daily environmental changes. Stomatal aperture is one of the many processes under circadian control; stomatal opening and closing occurs under constant conditions, even in the absence of environmental cues. To understand the significance of circadian-mediated anticipation in stomatal opening, we have generated SGC (specifically guard cell) Arabidopsis (Arabidopsis thaliana) plants in which the oscillator gene CIRCADIAN CLOCK ASSOCIATED1 (CCA1) was overexpressed under the control of the guard-cell-specific promoter, GC1. The SGC plants showed a loss of ability to open stomata in anticipation of daily dark-to-light changes and of circadian-mediated stomatal opening in constant light. We observed that under fully watered and mild drought conditions, SGC plants outperform wild type with larger leaf area and biomass. To investigate the molecular basis for circadian control of guard cell aperture, we used large-scale qRT-PCR to compare circadian oscillator gene expression in guard cells compared with the "average" whole-leaf oscillator and examined gene expression and stomatal aperture in several lines of plants with misexpressed CCA1. Our results show that the guard cell oscillator is different from the average plant oscillator. Moreover, the differences in guard cell oscillator function may be important for the correct regulation of photoperiod pathway genes that have previously been reported to control stomatal aperture. We conclude by showing that CONSTANS and FLOWERING LOCUS T, components of the photoperiod pathway that regulate flowering time, also control stomatal aperture in a daylength-dependent manner.

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Section: Discussion the Guard Cell Circadian System May Be Fine-tunedmentioning

confidence: 99%

mentioning

confidence: 99%

CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

et al. 2017

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“…For instance, early flowering lines have larger apertures, and this correlates with FT gene expression levels (Kinoshita et al ., ; Ando et al ., ). Vernalization also increases stomatal aperture (Kimura et al ., ), and this is associated with an increase in SOC1 and FT gene expression. Furthermore, overexpression of SOC1 alone is sufficient to increase stomatal opening (Kimura et al ., ).…”

Section: Examples Of Genetic Pleiotropy Of Flowering‐time Genes In Amentioning

confidence: 99%

Pleiotropy in developmental regulation by flowering‐pathway genes: is it an evolutionary constraint?

2019

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Summary Pleiotropy occurs when one gene influences more than one trait, contributing to genetic correlations among traits. Consequently, it is considered a constraint on the evolution of adaptive phenotypes because of potential antagonistic selection on correlated traits, or, alternatively, preservation of functional trait combinations. Such evolutionary constraints may be mitigated by the evolution of different functions of pleiotropic genes in their regulation of different traits. Arabidopsis thaliana flowering‐time genes, and the pathways in which they operate, are among the most thoroughly studied regarding molecular functions, phenotypic effects, and adaptive significance. Many of them show strong pleiotropic effects. Here, we review examples of pleiotropy of flowering‐time genes and highlight those that also influence seed germination. Some genes appear to operate in the same genetic pathways when regulating both traits, whereas others show diversity of function in their regulation, either interacting with the same genetic partners but in different ways or potentially interacting with different partners. We discuss how functional diversification of pleiotropic genes in the regulation of different traits across the life cycle may mitigate evolutionary constraints of pleiotropy, permitting traits to respond more independently to environmental cues, and how it may even contribute to the evolutionary divergence of gene function across taxa.

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“…5B)-DREBs are the most extensively studied drought-responsive group of genes in Arabidopsis (Sakuma et al 2002;Agarwal et al 2006;Xianjun et al 2011); (3) FT, a central gene in the flowering pathway (Fig. 5C)-several recent studies have implicated a physiological and genetic tie between drought-responsive and flowering pathways (McKay et al 2003;Lovell et al 2013Lovell et al , 2015Riboni et al 2013;Kimura et al 2015); (4) RLK1, the rice ortholog (OsLecRK4) which improves resistance to insect herbivory (Liu et al 2015), and (5) a rice ortholog (OSMate2) of the MATE efflux family gene AT1G71140, which, when overexpressed in A. thaliana, alters a variety of abiotic and biotic stress responses (Tiwari et al 2014). …”

Section: Trans and Trans-by-treatment Expression Regulatory Divergencementioning

confidence: 99%

Drought responsive gene expression regulatory divergence between upland and lowland ecotypes of a perennial C₄grass

et al. 2016

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Climatic adaptation is an example of a genotype-by-environment interaction (G×E) of fitness. Selection upon gene expression regulatory variation can contribute to adaptive phenotypic diversity; however, surprisingly few studies have examined how genome-wide patterns of gene expression G×E are manifested in response to environmental stress and other selective agents that cause climatic adaptation. Here, we characterize drought-responsive expression divergence between upland (drought-adapted) and lowland (mesic) ecotypes of the perennial C 4 grass, Panicum hallii, in natural field conditions. Overall, we find that cis-regulatory elements contributed to gene expression divergence across 47% of genes, 7.2% of which exhibit drought-responsive G×E. While less well-represented, we observe 1294 genes (7.8%) with trans effects. Trans-by-environment interactions are weaker and much less common than cis G×E, occurring in only 0.7% of trans-regulated genes. Finally, gene expression heterosis is highly enriched in expression phenotypes with significant G×E. As such, modes of inheritance that drive heterosis, such as dominance or overdominance, may be common among G×E genes. Interestingly, motifs specific to drought-responsive transcription factors are highly enriched in the promoters of genes exhibiting G×E and trans regulation, indicating that expression G×E and heterosis may result from the evolution of transcription factors or their binding sites. P. hallii serves as the genomic model for its close relative and emerging biofuel crop, switchgrass (Panicum virgatum). Accordingly, the results here not only aid in the discovery of the genetic mechanisms that underlie local adaptation but also provide a foundation to improve switchgrass yield under water-limited conditions.

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A Flowering Integrator, SOC1, Affects Stomatal Opening in Arabidopsis thaliana

Cited by 48 publications

References 57 publications

CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

Pleiotropy in developmental regulation by flowering‐pathway genes: is it an evolutionary constraint?

Drought responsive gene expression regulatory divergence between upland and lowland ecotypes of a perennial C₄grass

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A Flowering Integrator, SOC1, Affects Stomatal Opening in Arabidopsis thaliana

Cited by 48 publications

References 57 publications

CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and the Circadian Control of Stomatal Aperture

Pleiotropy in developmental regulation by flowering‐pathway genes: is it an evolutionary constraint?

Drought responsive gene expression regulatory divergence between upland and lowland ecotypes of a perennial C4grass

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Drought responsive gene expression regulatory divergence between upland and lowland ecotypes of a perennial C₄grass