A fluorescence-based optical fiber analyzer for catecholamine determination

Critical overview on the application of sensors and biosensors for clinical analysis

“…According to the authors, the proposed biosensor has several advantages such as low cost and simplicity of construction compared with biosensors based on isolated enzymes. Other sensors for the detection of epinephrine as well as other neurotransmitters such as dopamine and norepinephrine in urine samples are listed in Table 4, which use optical fibres with an alginate/laccase matrix [129] or fluorescence [130]. The three neurotransmitters (dopamine, epinephrine and norepinephrine) were tested in the sensors constructed by Silva et al [129] and Silva et al [130].…”

“…Other sensors for the detection of epinephrine as well as other neurotransmitters such as dopamine and norepinephrine in urine samples are listed in Table 4, which use optical fibres with an alginate/laccase matrix [129] or fluorescence [130]. The three neurotransmitters (dopamine, epinephrine and norepinephrine) were tested in the sensors constructed by Silva et al [129] and Silva et al [130]. A better LOD (0.00059-0.00088 ng mL −1 ) was reported for the fluorescence sensor compared with the optical fibre sensor based on the alginate/laccase matrix, with an LOD of 4.1-5.5 ng mL −1 .…”

“…When testing for dopamine, norepinephrine and epinephrine, figures of merit such as LOD (0.00059-0.00088 ng mL −1 ) and the linear range (between 0.001 and 0.3 ng mL −1 ) of the fluorescence optical fibre sensor were found to be superior to those of an HPLC-electrochemical detector (HPLC-ED) (0.0045-0.0051 ng mL −1 for the LOD and linear range between 0.005 and 0.125 ng mL −1 ). Thus, Silva et al [130] suggested the fluorescence sensor as a promising alternative to the current methodologies to determine catecholamines in clinical samples, due to its compact design, low-scale instrumentation and effective cost of analysis. Table 6 includes the figures of merit of current sensors and biosensors (studies published between 2011 and 2015) to compare their analytical performance in the detection of 1) pathogenic bacteria such as Escherichia coli, 2) viruses such as hepatitis C virus core antigen and avian influenza virus and 3) cancer cells.…”

Real-Time Dopamine Measurement in Awake Monkeys

“…Constant potential amperometry is a similar method, but it does not provide information about the ionic species contributing to the current being measured [13] . Optical measurements of DA are not well suited for deep brain use because of the probe size and light dispersion properties of the brain [14] . Positron emission tomography (PET) can be used to estimate DA concentration in voxels of a few cubic centimeters.…”

One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum

“…So it is of great importance to determine catecholamines in biological fluids (serum, plasma or urine). 1 In the literatures, a few analytical methods such as high-performance liquid chromatography (HPLC), 2,3 fluorescence measurements 4 and electrochemical analysis 5 have been reported for the determination of catecholamine in biological fluids. In all cases, sample preparation is essential, because there are many coexisting proteins in biological fluids, which generate interferences for further analysis.…”