A fluorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

Abstract

To monitor the levels of furaltadone in animal tissues, an immunochromatographic strip (immunostrip) based on quantum dot was developed for rapid detection of 3-amino-5methylmorpholino-2-oxazolidinone (AMOZ), the metabolite of furaltadone. Four monoclonal antibodies (mAbs) against nitrophenyl derivative of AMOZ (2-NP-AMOZ) were generated from the hybridoma cell lines. Among them, the mAb 5C10/3A8 showed the highest sensitivity to 2-NP-AMOZ. Using an immunostrip reader, with the 2-NP-AMOZ as the target, the immunostrip had a liner range from 1 to 30 ng/mL, with 50% inhibition concentration of 5.22 ng/mL and limit of detection of 0.07 ng/mL. The recoveries for AMOZ ranged from 78.6% to 108.9%, with coefficients of variation below 12%. A good correlation (R 2 = 0.9901) between the immunostrip and LC-MS/MS results in the tissues tested demonstrated the reliability of the immunostrip. These results indicate that proposed immunostrip is suitable for the rapid and quantitative detection of AMOZ residues in edible animal tissues. ARTICLE HISTORY

Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

“…From the above results, the test strip can be used for detecting the furaltadone metabolite AMOZ in catfish samples and is largely immune to matrix interference. When compared with previous immunoassays (Xie et al, 2019;Zhang et al, 2018) for the detection of AMOZ, this strip assay could detect AMOZ with a lower vLOD and cut-off value, and a greater sensitively. Moreover, the strip assay was more convenient and AMOZ spiked into the samples could be detected directly.…”

“…Immunoassays have the advantages of high sensitivity, rapid turnaround times, and high throughput (Gu et al, 2015;Li et al, 2020;Tan et al, 2020) and numerous studies have reported their use in the detection of AMOZ in aquatic animals, chicken, pork, beef, and eggs (Wang et al, 2019;Q. Wang et al, 2018;Xie et al, 2019). Although these immunoassays can detect AMOZ in a variety of sample types, detection limits are high.…”

Progress in immunoassays for nitrofurans detection

“…There was a good correlation (R 2 =0.9901) between the results given by the immunostrip and LC-MS/MS. Hence, the method could be used for the rapid and quantitative detection of AMOZ residues in edible animal tissues (Xie et al, 2019).…”

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…The amount of FM-mAb captured on the T and C lines is different; therefore, the lines present colours of different strength. The values of the T and C lines at different Cd concentrations is determined with a strip reader (Xie, Wu, Shi, & Le, 2019). The detection performance of the fluorescent test strip is evaluated by establishing a standard curve.…”