A fluorescent light-up probe with AIE characteristics for specific mitochondrial imaging to identify differentiating brown adipose cells

Gao, Meng; Sim, Choon Kiat; Leung, Chi Man; Hu, Qinglian; Feng, Guangxue; Xu, Feng; Tang, Ben Zhong; Liu, Bin

doi:10.1039/c4cc00452c

Cited by 103 publications

(81 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Confocal images showed that the probe could specially localize in mitochondria with bright blue emission, which Scheme 9 Chemical structure of the TPE-4Lac probe and schematic illustration for cholera toxin detection. 45 The probe utilised a salicylaldazine fluorophore with both AIE and ESIPT nature attached with two pyridinium groups for targeting mitochondria (Scheme 10). 9B-D).…”

Section: Mitochondria Trackingmentioning

confidence: 99%

Specific light-up bioprobes based on AIEgen conjugates

2015

Self Cite

View full text Add to dashboard Cite

Driven by the high demand for sensitive and specific tools for optical sensing and imaging, bioprobes with various working mechanisms and advanced functionalities are flourishing at an incredible speed. Conventional fluorescent probes suffer from the notorious effect of aggregation-caused quenching that imposes limitation on their labelling efficiency or concentration to achieve desired sensitivity. The recently emerged fluorogens with an aggregation-induced emission (AIE) feature offer a timely remedy to tackle the challenge. Utilizing the unique properties of AIE fluorogens (AIEgens), specific light-up probes have been constructed through functionalization with recognition elements, showing advantages such as low background interference, a high signal to noise ratio and superior photostability with activatable therapeutic effects. In this tutorial review, we summarize the recent progress in the development of specific AIEgen-based light-up bioprobes. Through illustration of their operation mechanisms and application examples, we hope to provide guidelines for the design of more advanced AIE sensing and imaging platforms with high selectivity, great sensitivity and wide adaptability to a broad range of biomedical applications.

show abstract

Section: Mitochondria Trackingmentioning

confidence: 99%

Specific light-up bioprobes based on AIEgen conjugates

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…27 Moreover, excited-state intramolecular proton transfer (ESIPT) mechanism was further introduced to AIE fluorogens, which allowed the development of light-up probes with 4 sharp imaging contrast, high signal-to-noise ratios and extremely large Stokes shifts. [28][29] In this contribution, we report a small apoptosis probe AIE-ZnDPA based on zinc coordination and salicylaldazine fluorophore skeleton. 30 The synthetic routes to AIE-DPA and AIE-ZnDPA are shown in Scheme S1.…”

Section: Introductionmentioning

confidence: 99%

A Cell Apoptosis Probe Based on Fluorogen with Aggregation Induced Emission Characteristics

Gao

Feng

et al. 2015

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

A fluorogen-based aggregation-induced emission zinc-dipicolylamine (AIE-ZnDPA) probe with aggregation-induced emission characteristics has been designed and synthesized to detect cell apoptosis. AIE-ZnDPA does not respond to healthy cells but selectively stains and lights up fluorescence in the membranes of early stage apoptotic cells as well as the nuclei of late stage apoptotic cells. Without zinc coordination, the precursor lipophilic AIE dipicolylamine (AIE-DPA) probe stains healthy cells and shows high affinity for lipid droplets (LDs).

show abstract

“…As MMP-dependent mitochondrial probes, these dyes are potentially applicable in tracking mitochondria for apoptosis studies, [23] identifying the differentiating brown adipose cells, [24] and evaluating sperm activity. [22] Moreover, it has been reported recently that most cancer cell lines exhibit a much higher MMP than normal cancer lines.…”

Section: Cancer Cell-targetingmentioning

confidence: 99%

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Jiang

Kwok

et al. 2017

Adv Funct Materials

Self Cite

100

View full text Add to dashboard Cite

Luminogens with aggregation-induced emission characteristics (AIEgens) are intriguing due to its rapid expansion in various high-tech applications. However, there is still in high demand on the development of novel AIEgens with easy preparation and functionalization, stable structures, tunable emissions, and high quantum efficiency. In this contribution, three AIEgens based on diphenyl isoquinolinium (IQ) derivatives are reported. They can be facilely synthesized and possess high structural stability, favorable visible light excitation, large Stokes shifts, high quantum yields, tunable colors, and sufficient two-photon absorption of near-infrared light. Importantly, they exhibit multifunctionalities. They exhibit mechanochromic property, making them capable to be applied for rewritable papers. They can also be applied in mitochondrial imaging with high specificity, cell permeability, brightness, biocompatibility, and photostability. They are promising for the applications in evaluation of mitochondrial membrane potential and image-guided cancer cell ablation. Last, they are able to stain bacteria in a wash-free manner. All these intriguing results suggest such readily accessible and multifunctional diphenyl IQ-based AIEgens provide a new platform for construction of advanced materials for practical applications.

show abstract

A fluorescent light-up probe with AIE characteristics for specific mitochondrial imaging to identify differentiating brown adipose cells

Cited by 103 publications

References 19 publications

Specific light-up bioprobes based on AIEgen conjugates

Specific light-up bioprobes based on AIEgen conjugates

A Cell Apoptosis Probe Based on Fluorogen with Aggregation Induced Emission Characteristics

Multifunctional AIEgens: Ready Synthesis, Tunable Emission, Mechanochromism, Mitochondrial, and Bacterial Imaging

Contact Info

Product

Resources

About