A fluorescent sensor recognized by the FA1 site for highly sensitive detection of HSA

Chao, Xi-Juan; Yao, Dezhi; Qi, Yongmei; Yuan, Cong; Huang, Dejun

doi:10.1016/j.aca.2021.339201

Cited by 17 publications

(9 citation statements)

References 74 publications

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“…The linear equation used was Δ R / R 0 = 0.27894 C HSA – 1.25345 ( R 2 = 0.99349). The detection limit (LOD) of the MHBios was computed by the formula LOD = 3σ/ S as 0.53 μg/mL. ,− …”

Section: Resultsmentioning

confidence: 99%

“…By contrasting the MHBios with other biosensors from the prior literature, the detection performance of the MHBios was demonstrated. ,, The LOD of the MHBios was many orders of magnitude lower than those of the NiFe 2 O 4 /paper-based magnetoelastic biosensor, fluorescent probe sensor, and antibody-functionalized gold nanorod sensor, all of which have wider detection ranges than the MHBios. , The MHBios not only has no label and is less expensive than the water-soluble BODIPY-based fluorescent probe but also offers higher sensitivity. Consequently, the MHBios has a lot of promise for identifying HSA (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…The detection limit (LOD) of the MHBios was computed by the formula LOD = 3σ/ S as 0.53 μg/mL. 2 , 25 − 27 …”

Section: Resultsmentioning

confidence: 99%

“…Kidney disease is a major concern because of its extremely high mortality rate and the complexity of detecting it early. Early detection can dramatically improve the effectiveness of disease treatment and prevention. − Microalbuminuria is a critical biomarker for assessing chronic kidney disease (CKD). The potency of human serum albumin (HSA) present in normal human urine is 20 mg/mL –1 .…”

Section: Introductionmentioning

confidence: 99%

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Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

et al. 2023

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A factor closely associated with renal disease status in clinical diagnosis is abnormal human serum albumin (HSA) concentration levels in human body fluids urine, serum, etc. The surface stress biosensor was developed as a new type of biosensor to detect protein molecule concentration and has a wide range of clinical applications. However, further sensitivity improvement is required to achieve higher detection performance. Herein, MXene/PDMS/ Fe 3 O 4 /PDMS of the multilayer heterogeneous membrane biosensor (MHBios) based on the coupling of the magnetic field, electric field, and surface stress field was successfully developed to achieve high sensitivity HSA detection through magnetic sensitization. The modified antibody specifically binds to HSA at the AuNP layer, allowing the biosensor to convert the surface stress caused by PDMS film deformation into an electrical signal. When the biosensor was exposed to a uniform magnetic field, the conductive path of the conductive layer was reshaped further as the magnetic force amplified the deformation of the PDMS film, enhancing the conversion of biological signals to electrical signals. The results exhibited that the detection limit (LOD) of the MHBios was 78 ng/mL when HSA concentration was 0−50 μg/ mL, which was markedly lower than the minimum diagnostic limit of microalbuminuria. Furthermore, the MHBios detected HSA in actual samples, confirming the potential for early disease screening.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…The detection limit (LOD) of the MHBios was computed by the formula LOD = 3σ/ S as 0.53 μg/mL. 2 , 25 − 27 …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

et al. 2023

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“…There are multiple domains in the structure of albumin and different domains can bind with the different properties of drugs. Both BSA and HSA have three identical domains, and HSA has two major albumin binding sites, known as Sudlow site I and II, which can efficiently bind with hydrophobic drugs and deliver to the cancer site 147 . Due to the functional groups of the side chain, multifunctional albumin nanoparticles could include modified amine groups with carboxyl groups 139 .…”

Section: Backbones and Structures Of Multifunctional Nanoparticlesmentioning

confidence: 99%

Multifunctional nanoparticle for cancer therapy

Wang

Zhang

Duan

et al. 2023

MedComm

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Cancer is a complex disease associated with a combination of abnormal physiological process and exhibiting dysfunctions in multiple systems. To provide effective treatment and diagnosis for cancer, current treatment strategies simultaneously focus on various tumor targets. Based on the rapid development of nanotechnology, nanocarriers have been shown to exhibit excellent potential for cancer therapy. Compared with nanoparticles with single functions, multifunctional nanoparticles are believed to be more aggressive and potent in the context of tumor targeting. However, the development of multifunctional nanoparticles is not simply an upgraded version of the original function, but involves a sophisticated system with a proper backbone, optimized modification sites, simple preparation method, and efficient function integration. Despite this, many well‐designed multifunctional nanoparticles with promising therapeutic potential have emerged recently. Here, to give a detailed understanding and analyzation of the currently developed multifunctional nanoparticles, their platform structures with organic or inorganic backbones were systemically generalized. We emphasized on the functionalization and modification strategies, which provide additional functions to the nanoparticle. We also discussed the application combination strategies that were involved in the development of nanoformulations with functional crosstalk. This review thus provides an overview of the construction strategies and application advances of multifunctional nanoparticles.

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Fluorogenic gemcitabine based light up sensor for serum albumin detection in complex biological matrices

Gadly

Patro

Chakraborty

2022

Colloids and Surfaces B: Biointerfaces

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A fluorescent sensor recognized by the FA1 site for highly sensitive detection of HSA

Cited by 17 publications

References 74 publications

Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection

Multifunctional nanoparticle for cancer therapy

Fluorogenic gemcitabine based light up sensor for serum albumin detection in complex biological matrices

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