A Functional Genomic Study to Identify Differential Gene Expression in the Preterm and Term Human Myometrium1

Charpigny, Gilles; Leroy, Marie-Josèphe; Breuiller-Fouché, Michelle; Tanfin, Zarha; Mhaouty-Kodja, Sakina; Robin, Philippe; Leiber, Denis; Cohen‐Tannoudji, Joëlle; Cabrol, D.; Barberis, Claude; Germain, Guy

doi:10.1095/biolreprod.102.013763

Cited by 95 publications

(83 citation statements)

References 38 publications

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“…Functional genomics has arrived at the study of parturition in different species: mice (Bethin et al 2003, Salomonis et al 2005; rats (Girotti & Zingg 2003) and humans (Chan et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Bethin et al 2003, Charpigny et al 2003, Keelan et al 2003, Esplin et al 2005, Havelock et al 2005. Also in different tissues: myometrium (Aguan et al 2000, Chan et al 2002, Bethin et al 2003, Charpigny et al 2003, Esplin et al 2005, Havelock et al 2005, Salomonis et al 2005; primary cultures of human myometrial cells ; fetal membranes (Muhle et al 2001, Chen et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Keelan et al 2003 and trophoblasts (Chen et al 2002, Kato et al 2002.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…Also in different tissues: myometrium (Aguan et al 2000, Chan et al 2002, Bethin et al 2003, Charpigny et al 2003, Esplin et al 2005, Havelock et al 2005, Salomonis et al 2005; primary cultures of human myometrial cells ; fetal membranes (Muhle et al 2001, Chen et al 2002, Marvin et al 2002a, 2002b, Tashima et al 2002, Keelan et al 2003 and trophoblasts (Chen et al 2002, Kato et al 2002. Various methodologies have been used: suppression subtractive hybridization (SSH) (Chan et al 2002) or microarrays for most of the previous cited studies.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…It gives an overall picture of the changes that the uterine muscle undergoes in its adaptation to gestation. Two studies have described the changes in gene expression in the myometrium in preterm patients not in labor (PTNIL) versus patients at TNIL (Bethin et al 2003, Charpigny et al 2003 and two additional studies have described the changes in gene expression in the myometrium in patients at TNIL versus patients at term in labor (TIL) (Esplin et al 2005, Havelock et al 2005. Other studies could not be compiled directly, either because they did not provide sufficient information on the genes being studied (i.e.…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…KEGG is a knowledge base for systematic analysis of gene functions, linking genomic information with higher order functional information (http://www.genome.jp/kegg/pathway.html). Because relevant raw data for myometrium microarrays were not always available, input data files for use with the Onto-Tools software modules were built on gene lists reported in published papers or, when available, on the complete gene expression data set, deposited as supplemental data at a public Internet site, see Bethin et al 2003, Charpigny et al 2003, Rehman et al 2003. Drawbacks and limitations inherent to the use of Onto-Tools or of their cognate alternatives for ontological analysis, have been reviewed .…”

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

“…This leads to the idea that increased endogenous ROCK1 activity, resulting in enhanced RhoAmediated calcium sensitization, is involved in the increased contractility at the time of labor. It is of note that epidermal growth factor (EGF) increases the presence of actin and myometrial EGF receptor transcripts are increased at the time of parturition in humans (Gargiulo et al 1997, Charpigny et al 2003, Rehman et al 2003.…”

Section: Regulation Of Actin Cytoskeletonmentioning

confidence: 99%

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Gene and protein expression in the myometrium in pregnancy and labor

Breuiller-Fouché¹,

Germain²

2006

Reproduction

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Microarray technologies widen our comprehension of the major structural and metabolic transformations which affect the myometrium from the very beginning of pregnancy until parturition. The results are coherent with the mass of information which was accumulated previously, primarily on the basis of studies of selected critical factors. They highlight the activation of precise signaling pathways, some of which may have been previously under evaluated. The remodelling and maturation processes that the myometrium undergoes in pregnancy appear clearly as phenomena which last during the full course of gestation. Comparatively, the onset of labor is perhaps the phenomenon which remains the least well described by these methods of analysis. Nevertheless, genomic studies constitute a necessary first step of orientation and help establishing new links between the generic signaling pathways that are activated during the normal or pathological gestation. These studies also represent an indicative step that will have to be paralleled, in the future, with the results of the systematic proteomic analysis of the myometrium.

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Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Functional Genomic Studies During Pregnancymentioning

confidence: 99%

Section: Regulation Of Actin Cytoskeletonmentioning

confidence: 99%

See 3 more Smart Citations

Gene and protein expression in the myometrium in pregnancy and labor

Breuiller-Fouché¹,

Germain²

2006

Reproduction

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Current awareness on comparative and functional genomics

2003

Comp Funct Genom

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on comparative and functional genomics. Each bibliography is divided into 16 sections. 1 Reviews & symposia; 2 General; 3 Large‐scale sequencing and mapping; 4 Genome evolution; 5 Comparative genomics; 6 Gene families and regulons; 7 Pharmacogenomics; 8 Large‐scale mutagenesis programmes; 9 Functional complementation; 10 Transcriptomics; 11 Proteomics; 12 Protein structural genomics; 13 Metabolomics; 14 Genomic approaches to development; 15 Technological advances; 16 Bioinformatics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted

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Integrating single‐cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour

Chen

Wang

et al. 2023

Clinical & Translational Med

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Background The transition of the myometrium from a quiescent to a contractile state during labour is known to involve inflammation, which is characterized by the infiltration of immune cells and the secretion of cytokines. However, the specific cellular mechanisms underlying inflammation in the myometrium during human parturition are not yet fully understood. Methods Through the analysis of transcriptomics, proteomics, and cytokine arrays, the inflammation in the human myometrium during labour was revealed. By performing single‐cell RNA sequencing (scRNA‐seq) and spatiotemporal transcriptomic (ST) analyses on human myometrium in term in labour (TIL) and term in non‐labour (TNL), we established a comprehensive landscape of immune cells, their transcriptional characteristics, distribution, function and intercellular communications during labour. Histological staining, flow cytometry, and western blotting were applied to validate some results from scRNA‐seq and ST. Results Our analysis identified immune cell types, including monocytes, neutrophils, T cells, natural killer (NK) cells and B cells, present in the myometrium. TIL myometrium had a higher proportion of monocytes and neutrophils than TNL myometrium. Furthermore, the scRNA‐seq analysis showed an increase in M1 macrophages in TIL myometrium. CXCL8 expression was mainly observed in neutrophils and increased in TIL myometrium. CCL3 and CCL4 were principally expressed in M2 macrophages and neutrophils‐6, and decreased during labour; XCL1 and XCL2 were specifically expressed in NK cells, and decreased during labour. Analysis of cytokine receptor expression revealed an increase in IL1R2, which primarily expressed in neutrophils. Finally, we visualized the spatial proximity of representative cytokines, contraction‐associated genes, and corresponding receptors in ST to demonstrate their location within the myometrium. Conclusions Our analysis comprehensively revealed changes in immune cells, cytokines, and cytokine receptors during labour. It provided a valuable resource to detect and characterize inflammatory changes, yielding insights into the immune mechanisms underlying labour.

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A Functional Genomic Study to Identify Differential Gene Expression in the Preterm and Term Human Myometrium1

Cited by 95 publications

References 38 publications

Gene and protein expression in the myometrium in pregnancy and labor

Gene and protein expression in the myometrium in pregnancy and labor

Current awareness on comparative and functional genomics

Integrating single‐cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour

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