A Functional Hydrogel Bead-Based High-Throughput Screening System for Mammalian Cells with Enhanced Secretion of Therapeutic Antibodies
Diah Anggraini Wulandari,
Kyosuke Tsuru,
Kosuke Minamihata
et al.
Abstract:Droplet-based high-throughput screening systems are an emerging technology that provides a quick test to screen millions of cells with distinctive characteristics. Biopharmaceuticals, specifically therapeutic proteins, are produced by culturing cells that secrete heterologous recombinant proteins with different populations and expression levels; therefore, a technology to discriminate cells that produce more target proteins is needed.Here, we present a droplet-based microfluidic strategy for encapsulating, scr… Show more
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