A functional polymorphism of PON1 interferes with microRNA binding to increase the risk of ischemic stroke and carotid atherosclerosis

Liu, Mu En; Liao, Yi‐Chu; Lin, Ruey Tay; Wang, Yung Song; Hsi, Edward; Lin, Hsiu Fen; Chen, Ku Chung; Juo, Suh Hang Hank

doi:10.1016/j.atherosclerosis.2013.01.036

Cited by 62 publications

(42 citation statements)

References 37 publications

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“…[1][2][3] Numerous in-vitro and in-vivo studies have shown that a low average level, large gradients and high oscillation of wall shear stress (WSS) contribute to intima-media thickening and carotid atherosclerosis. [4][5][6][7][8] The Hagen-Poiseuille equation is the most commonly-used calculation method for WSS assessment.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Regional Plaque Characteristics Based on Quantitative Wall Shear Stress Distribution Analysis Using Color Doppler Flow Imaging

Zhang¹,

Ding²,

Xia³

et al. 2016

j med imaging hlth inform

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Objective: The purpose of this study is to explore the feasibility of assessing wall shear stress (WSS) spatial distribution around plaque and estimation of its characteristics. Methods: Fifty patients with intima-media thickening were prospectively enrolled and were divided into an IMT group. At the same time, another fifty healthy volunteers were enrolled as the control group. In addition, five patients with serious plaque condition were enrolled. All the subjects were scanned and the DICOM files were imported into our in-house program. The mean WSS values and correlation analyses from one hundred subjects were compared with those calculated by the HagenPoiseuille equation in order to check the accuracy of the churned data. After validation, the WSS distributions of regional plaques were assessed by this program. Results: The mean WSS value calculated by Hagen-Poiseuille equation was 3.12 ± 0.65 dyne/cm 2 , while it was 2.87 ± 0.59 dyne/cm 2 by the program. The difference was not statistically significant (t = −2 069, P = 0 496). Similar to the Hagen-Poiseuille equation, a negative liner correlation was also found between the calculated WSS and intima-media thickness (r = −0 806, P = 0 000). Based on our developed platform, we found that the WSS distribution at the middle of the proximal plaque shoulder was larger than the top of the shoulder. Conclusions: This study demonstrated that our in-house program can be utilized to assess WSS near plaque shoulders accurately and efficiently.

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Section: Introductionmentioning

confidence: 99%

Estimation of Regional Plaque Characteristics Based on Quantitative Wall Shear Stress Distribution Analysis Using Color Doppler Flow Imaging

Zhang¹,

Ding²,

Xia³

et al. 2016

j med imaging hlth inform

View full text Add to dashboard Cite

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“…A miR-SNP can interfere with miR's binding to its target gene to affect gene expression. Our recent works have shown that miR-SNPs at the paraoxonase 1 (PON1) and matrix metalloproteinase-2 (MMP-2) genes can affect the respective genes leading to increase risks for atherosclerosis and endometriosis (Liu et al, 2013;Tsai et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-765 influences arterial stiffness through modulating apelin expression

Liao

Wang

Hsi

et al. 2015

Molecular and Cellular Endocrinology

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“…PON1 was identified as a target gene of miR-616, and a correlation between the TC genotype and a markedly reduced risk of both thinner intima media thickness (IMT) and ischemic stroke was found in the Han Chinese population [12]. Liu et al also suggested that the SNP rs3735590 (C>T) in human could affect the binding of miR-616 to PON1, thus leading to a higher risk of carotid atherosclerosis and ischemic stroke [12]. In our study, miR-616 inhibited PON1 in primary hepatocytes genotyped as CC.…”

Section: Rs3735590 Impaired the Inhibitory Effect Of Mir-616 On Pon1 mentioning

confidence: 99%

“…PON1 is related to the prognosis in patient undergoing CABG, whereas a polymorphism (rs3735590) has been located in the miR-616 binding site within the 3'UTR of PON1 [7,12]. The aim of this study was to investigate whether rs3735590 polymorphism could be used as a prognosis marker in COPD patients undergoing CABG.…”

Section: Introductionmentioning

confidence: 99%

Identification of Prognostic Value of Rs3735590 Polymorphism in 3’-Untranslated Region (3’-UTR) of Paraoxonase 1 (PON-1) in Chronic Obstructive Pulmonary Disease Patients who Received Coronary Artery Bypass Grafting (CABG)

Sun

Chen

2018

Cell Physiol Biochem

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Background/Aims: In the treatment of serious and symptomatic coronary heart disease (CHD), coronary artery bypass grafting (CABG) is a frequently utilized intervention. In addition, the risk of CHD is strongly associated with the low activity of paraoxonase-1 (PON1), whose 3’-UTR harbors an rs3735590 polymorphism. The aim of this study was to investigate whether the rs3735590 polymorphism could be used as a prognosis marker in chronic obstructive pulmonary disease (COPD) patients undergoing CABG. In addition, the hypothesis, i.e., the rs3735590 polymorphism may be involved in the regulation of PON1 gene expression via modulating its interaction with miRNAs, was tested in this study. Methods: 292 patients diagnosed with COPD and treated with CABG were recruited for this study. Genomic DNA was extracted from clinical samples, and real-time quantitative PCR and Western-blot were used to measure the expression of miR-616 and PON1 in liver cells of different genotypes. Results: 292 COPD patients were divided into three groups according to their genotypes, i.e., rs3735590: CC (212), TC (75), and TT (5), respectively (TC and TT were merged in one group of T carriers for statistical analyses). Patients with the CC genotype were associated with a shorter event-free survival time as compared to patients with the T genotypes. In addition, PON1 was confirmed as a direct target gene of miR-616, while experiments with primary cells of different genotypes showed that miR-616 inhibited the expression of PON1 in CC cells. On the contrary, rs3735590 impaired such inhibitory effect of miR-616 in TT cells. Conclusion: The rs3735590 polymorphism of PON1 acts as a prognostic biomarker in COPD patients treated by CABG.

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A functional polymorphism of PON1 interferes with microRNA binding to increase the risk of ischemic stroke and carotid atherosclerosis

Cited by 62 publications

References 37 publications

Estimation of Regional Plaque Characteristics Based on Quantitative Wall Shear Stress Distribution Analysis Using Color Doppler Flow Imaging

Estimation of Regional Plaque Characteristics Based on Quantitative Wall Shear Stress Distribution Analysis Using Color Doppler Flow Imaging

MicroRNA-765 influences arterial stiffness through modulating apelin expression

Identification of Prognostic Value of Rs3735590 Polymorphism in 3’-Untranslated Region (3’-UTR) of Paraoxonase 1 (PON-1) in Chronic Obstructive Pulmonary Disease Patients who Received Coronary Artery Bypass Grafting (CABG)

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