A G-quadruplex aptamer based impedimetric sensor for free lysine and arginine

Carter, Z.A.; Kataky, Ritu

doi:10.1016/j.snb.2016.12.010

Cited by 29 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Disposable gold screen printed electrodes are already available on the market as an alternative to the conventional electrodes. More recently, gold-sputtered microelectrodes have become commercially available as an attractive platform for the development of miniaturized biosensors through covalent attachment of biomolecules to the gold surface via thiols [1][2][3][4][5] . These microelectrodes are made up of gold, sputtered with a thickness of 10-500 nm on a glass substrate directly or through an intermediate layer of chromium for better adhesion 1 .…”

Section: Introductionmentioning

confidence: 99%

Gold-sputtered microelectrodes with built-in gold reference and counter electrodes for electrochemical DNA detection

Shanmugam

Trashin

Wael

2020

Analyst

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Gold-sputtered microelectrodes with built-in gold reference and counter electrodes for electrochemical DNA detection

Shanmugam

Trashin

Wael

2020

Analyst

View full text Add to dashboard Cite

show abstract

“…However, depending on the type of aptamer its binding affinity is subject to change as a function of conditions. In our case the presence of K + or Mg 2+ may interfere (Carter and Kataky, 2017;Fiegland et al, 2012;Noireaux, 2012, 2010).…”

Section: Introductionmentioning

confidence: 65%

“…Furthermore antibodies are sensitive to chemical conditions (Jayasena, 1999;Song et al, 2008). A lysine aptamer can bind very specifically against lysine (Carter and Kataky, 2017;Fiegland et al, 2012;Sudarsan et al, 2003). However, depending on the type of aptamer its binding affinity is subject to change as a function of conditions.…”

Section: Introductionmentioning

confidence: 99%

A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems

Finkler

Kurt

Hartz

et al. 2020

Preprint

View full text Add to dashboard Cite

Cell-free transcription-translation systems are a versatile tool to study gene expression, enzymatic reactions and biochemical regulation mechanisms. Because cell-free transcription-translation systems are often derived from cell lysates, many different substances, among them amino acids, are present. However, experiments concerning the incorporation of non-canonical amino acids into proteins require a system with negligible amounts of canonical analogs. Here we propose a two-step method for the removal of residual free lysine in an all Escherichia coli-based cell-free expression system. The first step consists of the expression of a high-lysine dummy protein. The second step consists of direct removal via binding between lysine and DNA. The presented method is an efficient, fast and simple way to remove residual lysine without altering the system ability to perform gene expression.

show abstract

“…It was reported that G-quadruplex DNA or RNA could fold into a stable structure and bind to various targets in vivo or in vitro [ 71 , 72 ]. Some aptamers with G-quadruplex motifs were also reported to bind to small molecules with high affinity, such as GTX [ 58 ], aflatoxin [ 73 ], lysine, arginine [ 74 ], etc.…”

Section: Resultsmentioning

confidence: 99%

Selection, Characterization, and Optimization of DNA Aptamers against Challenging Marine Biotoxin Gymnodimine-A for Biosensing Application

Zhang

Gao²,

Deng³

et al. 2022

Toxins

View full text Add to dashboard Cite

Gymnodimines (GYMs), belonging to cyclic imines (CIs), are characterized as fast-acting toxins, and may pose potential risks to human health and the aquaculture industry through the contamination of sea food. The existing detection methods of GYMs have certain defects in practice, such as ethical problems or the requirement of complicated equipment. As novel molecular recognition elements, aptamers have been applied in many areas, including the detection of marine biotoxins. However, GYMs are liposoluble molecules with low molecular weight and limited numbers of chemical groups, which are considered as “challenging” targets for aptamers selection. In this study, Capture-SELEX was used as the main strategy in screening aptamers targeting gymnodimine-A (GYM-A), and an aptamer named G48nop, with the highest KD value of 95.30 nM, was successfully obtained by screening and optimization. G48nop showed high specificity towards GYM-A. Based on this, a novel aptasensor based on biolayer interferometry (BLI) technology was established in detecting GYM-A. This aptasensor showed a detection range from 55 to 1400 nM (linear range from 55 to 875 nM) and a limit of detection (LOD) of 6.21 nM. Spiking experiments in real samples indicated the recovery rate of this aptasensor, ranging from 96.65% to 109.67%. This is the first study to report an aptamer with high affinity and specificity for the challenging marine biotoxin GYM-A, and the new established aptasensor may be used as a reliable and efficient tool for the detection and monitoring of GYMs in the future.

show abstract

A G-quadruplex aptamer based impedimetric sensor for free lysine and arginine

Cited by 29 publications

References 35 publications

Gold-sputtered microelectrodes with built-in gold reference and counter electrodes for electrochemical DNA detection

Gold-sputtered microelectrodes with built-in gold reference and counter electrodes for electrochemical DNA detection

A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems

Selection, Characterization, and Optimization of DNA Aptamers against Challenging Marine Biotoxin Gymnodimine-A for Biosensing Application

Contact Info

Product

Resources

About