A Ga3+self-assembled fluorescent probe for ATP imaging in vivo

Xiao, Liangliang; Sun, Shiguo; Pei, Zhichao; Pei, Yuxin; Pang, Yi; Xu, Yongqian

doi:10.1016/j.bios.2014.10.038

Cited by 32 publications

(6 citation statements)

References 40 publications

(62 reference statements)

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“…The Bzx‐BDI conjugates merged the properties of both moieties. On the one hand, the 2‐phenylbenzoxazole fragment bore its chemical and photochemical stability together with excellent optical properties that make it the basis of widely used fluorescent materials . It must be underlined that this motif leads to many derivatives that are strongly emissive in the solid state and sometimes display AIE behavior .…”

Section: Introductionmentioning

confidence: 99%

Conjugates of Benzoxazole and GFP Chromophore with Aggregation‐Induced Enhanced Emission: Influence of the Chain Length on the Formation of Particles and on the Dye Uptake by Living Cells

Carayon

Ghodbane

Gibot

et al. 2016

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Six conjugates of benzoxazole and green fluorescent protein chromophore that differ by the length of their alkyl chain (from C1 to C16) are investigated. They exhibit rigidofluorochromism and clear aggregation-induced emission enhancement (AIEE) behavior with emission in the orange-red that is specific to the solid state. A preparation method based on solvent exchange is used to prepare particles. The self-association properties of these molecules depend on the length of the alkyl chain. Microfibers, platelets, and rounded microparticles are successively obtained by increasing the chain length. The same method is used to prepare nanoparticles (NPs) that are fully characterized. In particular, homogeneous populations of stable NPs measuring around 70 nm are obtained with the analogs whose chains contain four to eight carbon atoms. The behavior with respect to living cells is also influenced by the nature of the compounds. Only the dyes with intermediate hydrophobicity are efficiently uptaken by both normal and tumor cells, and fluorescence only originates from dispersed dye molecules. There is no evidence for incorporation of NPs into cells. This work shows that small variations of the chemical structure must be taken into account for making the best use of AIEE compounds in view of precise applications.

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Section: Introductionmentioning

confidence: 99%

Conjugates of Benzoxazole and GFP Chromophore with Aggregation‐Induced Enhanced Emission: Influence of the Chain Length on the Formation of Particles and on the Dye Uptake by Living Cells

Carayon

Ghodbane

Gibot

et al. 2016

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“…Consequently, the study of ATP detection method has important clinical significance. With high selectivity and sensitivity (Xiao et al 2015), fluorescent biosensors have been widely used in the detection of nucleic acids, ions, proteins, and organic molecules (Li et al 2017;Qiu et al 2013;Wang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Zhang

et al. 2021

J Anal Sci Technol

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Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) and adenosine triphosphate (ATP) as input signal and fluorescence signal as output signal, if single-strand DNA (DNA-L), single-strand DNA (DNA-S), and ATP were present at the same time, one segment of DNA-L formed a hairpin ring with ATP, and the other segment of DNA-L formed a completely complementary hairpin stem with DNA-S. The hairpin DNA was detached from the GO surface, and PicoGreen dye was embedded into the hairpin stem, and the fluorescence signal was enhanced. The molecular logic gate was constructed through the establishment of logic histogram, logic circuit, truth table, and logic formula. The biosensor-related performances including sensitivity, selectivity, and linearity were investigated, respectively. Results We have successfully constructed a AND logic gate. The detection limit of ATP is 138.0 pmol/L (3σ/slope) with detection range of 50–500 nmol/L (R2 = 0.98951), and its sensitivity is 4.748 × 106–6.875 × 108 a.u. (mol/L)−1. Conclusions The logic gate hairpin aptamer sensor has the advantages of high sensitivity, low detection limit, and low cost, and can be successfully applied to the detection of adenosine triphosphate (ATP) in actual human urine samples.

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“…The reported approaches for the detection of ATP require specialized and/or costly equipment such as HPLC, 1 NMR, 2 or micro-uidic chips. 3 To address these limitations, various uorescent probes such as low molecular weight, [4][5][6] polymer, 7,8 and supramolecular sensors 9 that enable simpler and efficient detection have been developed. However, these existing strategies have disadvantages such as the necessity for expensive supported metals or proteins, and also suffer from poor sensitivities and selectivities.…”

Section: Introductionmentioning

confidence: 99%

Phosphate-sensing with (di-(2-picolyl)amino)quinazolines based on a fluorescence on–off system

et al. 2020

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A Ga3+self-assembled fluorescent probe for ATP imaging in vivo

Cited by 32 publications

References 40 publications

Conjugates of Benzoxazole and GFP Chromophore with Aggregation‐Induced Enhanced Emission: Influence of the Chain Length on the Formation of Particles and on the Dye Uptake by Living Cells

Conjugates of Benzoxazole and GFP Chromophore with Aggregation‐Induced Enhanced Emission: Influence of the Chain Length on the Formation of Particles and on the Dye Uptake by Living Cells

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Phosphate-sensing with (di-(2-picolyl)amino)quinazolines based on a fluorescence on–off system

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