“…injection. The mean survival time and percentage of increment in life span (% ILS) was calculated as previously reported [25], [32].Figure 1Schematic for in vivo DLA and EAC ascites tumor models.Female mice were intraperitoneally transplanted with DLA or EAC ascites tumors on day 1 or day 8 and divided into four groups as shown, depending on the treatment schedule. Each of these groups has been subdivided into four subgroups (n = 6/subgroup) – Control (PBS), PST001, Dox and PST-Dox.…”
Section: Methodsmentioning
confidence: 99%
“…Animals were sacrificed on day 23 to determine tumor volume and overall survival (n = 6/subgroup). The radii of the developing tumors were measured every third day from day 7 to day 31, using vernier calipers and the tumor volume was estimated using the formula: V = 4/3 π r 1 2 r 2 , where r 1 and r 2 represent the radii from two different sites [25], [32], [33].…”
Doxorubicin (Dox) treatment is limited by severe toxicity and frequent episodes of treatment failure. To minimize adverse events and improve drug delivery efficiently and specifically in cancer cells, encapsulation of Dox with naturally obtained galactoxyloglucan polysaccharide (PST001), isolated from Tamarindus indica was attempted. Thus formed PST-Dox nanoparticles induced apoptosis and exhibited significant cytotoxicity in murine ascites cell lines, Dalton’s lymphoma ascites and Ehrlich’s ascites carcinoma. The mechanism contributing to the augmented cytotoxicity of nanoconjugates at lower doses was validated by measuring the Dox intracellular uptake in human colon, leukemic and breast cancer cell lines. PST-Dox nanoparticles showed rapid internalization of Dox into cancer cells within a short period of incubation. Further, in vivo efficacy was tested in comparison to the parent counterparts - PST001 and Dox, in ascites and solid tumor syngraft mice models. Treatment of ascites tumors with PST-Dox nanoparticles significantly reduced the tumor volume, viable tumor cell count, and increased survival and percentage life span in the early, established and prophylactic phases of the disease. Administration of nanoparticles through intratumoral route delivered more robust antitumor response than the intraperitoneal route in solid malignancies. Thus, the results indicate that PST-Dox nanoparticles have greater potential compared to the Dox as targeted drug delivery nanocarriers for loco regional cancer chemotherapy applications.
“…injection. The mean survival time and percentage of increment in life span (% ILS) was calculated as previously reported [25], [32].Figure 1Schematic for in vivo DLA and EAC ascites tumor models.Female mice were intraperitoneally transplanted with DLA or EAC ascites tumors on day 1 or day 8 and divided into four groups as shown, depending on the treatment schedule. Each of these groups has been subdivided into four subgroups (n = 6/subgroup) – Control (PBS), PST001, Dox and PST-Dox.…”
Section: Methodsmentioning
confidence: 99%
“…Animals were sacrificed on day 23 to determine tumor volume and overall survival (n = 6/subgroup). The radii of the developing tumors were measured every third day from day 7 to day 31, using vernier calipers and the tumor volume was estimated using the formula: V = 4/3 π r 1 2 r 2 , where r 1 and r 2 represent the radii from two different sites [25], [32], [33].…”
Doxorubicin (Dox) treatment is limited by severe toxicity and frequent episodes of treatment failure. To minimize adverse events and improve drug delivery efficiently and specifically in cancer cells, encapsulation of Dox with naturally obtained galactoxyloglucan polysaccharide (PST001), isolated from Tamarindus indica was attempted. Thus formed PST-Dox nanoparticles induced apoptosis and exhibited significant cytotoxicity in murine ascites cell lines, Dalton’s lymphoma ascites and Ehrlich’s ascites carcinoma. The mechanism contributing to the augmented cytotoxicity of nanoconjugates at lower doses was validated by measuring the Dox intracellular uptake in human colon, leukemic and breast cancer cell lines. PST-Dox nanoparticles showed rapid internalization of Dox into cancer cells within a short period of incubation. Further, in vivo efficacy was tested in comparison to the parent counterparts - PST001 and Dox, in ascites and solid tumor syngraft mice models. Treatment of ascites tumors with PST-Dox nanoparticles significantly reduced the tumor volume, viable tumor cell count, and increased survival and percentage life span in the early, established and prophylactic phases of the disease. Administration of nanoparticles through intratumoral route delivered more robust antitumor response than the intraperitoneal route in solid malignancies. Thus, the results indicate that PST-Dox nanoparticles have greater potential compared to the Dox as targeted drug delivery nanocarriers for loco regional cancer chemotherapy applications.
“…These experiments were performed as described earlier [22]. The cells were observed under an inverted fluorescent microscope, using a FITC filter (Olympus 1X51, Singapore) to view the apoptotic or non-apoptotic cells.…”
“…The composition of the essential oils (Grosso et al, 2007), as well as the role of the flavonoids from P. tridentatum water extracts as protectors against endothelial oxidative injury have been investigated (Vitor et al, 2004). However, as far as we know, the polysaccharide Aravind, George, Varghese, & Sreelekha, 2013;Rosário et al, 2008;Rosário, Kangussu-Marcolino, do Amaral, Noleto, & Petkowicz, 2011;Simões, Nunes, Domingues, & Coimbra, 2010;Simões, Nunes, Domingues, Coimbra, & Domingues, 2012).…”
a b s t r a c tPterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60-75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endopolygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.
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