A gamma‐ray detector for capillary zone electrophoresis and its use in the analysis of some radiopharmaceuticals

Abstract

The construction and evaluation of a gamma-ray detector for capillary zone electrophoresis is described. The detector was shown to have a linear response from the limit of detection 10 to 500 Bqcm-3 corresponding to 5.1 x 10(-17) to 2.55 x 10(-15) gcm-3 43Tc99m. The application of the detector for the analysis of some radiopharmaceuticals is presented.

Sensitivity Enhancement for Capillary Electrophoresis

“…By using a variety of configurations (41,42) researchers have obtained sensitivities on the order of 10" 16 -10 -18 moles of radioactive label (10~9-10 -n M 32p. Radiochemical detection has often been effective when extremely high sensitivity has been required, and it is still widely used for HPLC.…”

On the separation of99mTcO4-,99mTc-DTPA and99mTc-citrate as marker species for the determination of Tc chemical forms in plant material using capillary zone electrophoresis

“…The detection efficiency as function of compound velocity was determined experimentally, using 99mTc0 4 -in CZE under various applied voltages (Figure 4 ). The latter efficiency is not reported by the pioneers of 99mTc-analysis in CZE, which may be the reason for the absence of 99mTc-recovery data in the paper by Altria et al ( 1990). The line drawn in Figure 4, is generated by fitting the function y =a X z-1 , withy= detector response and z = compound velocity.…”

“…Capillary zone electrophoresis (CZE) was selected as the separation method to investigate, because CZE gives the opportunity to repress strongly column (i.e. capillary wall) interactions with relatively labile Tc-complexes, and CZE shows superior separation characteristics compared to, e.g., HPLC techniques (Altria et al, 1990).…”

Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser

“…Na eletroforese capilar estão disponíveis detectores que são, basicamente, a adaptação dos detectores utilizados nos equipamentos de cromatografia em meio líquido para o formato capilar, e correspondente tempo rápido de resposta. Tais detectores podem ser: de absorção no UV-vis 14 , fluorescência 15 , fluorescência induzida por laser 16 , espectrometria de massas 17 , condutividade 18 , amperometria 19 , radioatividade 20 , índice de refração 21 , dicroísmo circular 22 e Raman 23 .…”