A Gene Encoding Cold-Circadian Rhythm-RNA Binding-Like Protein (CCR-Like) from Upland Cotton (Gossypium hirsutum L.) Confers Tolerance to Abiotic Stresses in Transgenic Tobacco

The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

“…Finger millet seedlings at the three-leaf growth stage were subjected to different abiotic stressors, including heat at 42 °C, dehydration by removing seedlings from the medium and keeping them on 3-mm Whatman sheet, sodium chloride (NaCl; 250 mM), mannitol (300 mM), abscisic acid (ABA; 100 µM), hydrogen peroxide (H 2 O 2 ; 25 mM), and DTT (2.5 mM) 47 , 64 , 65 . Seedling samples were collected after 2 h, 4 h, 6 h, 8 h, and 24 h of treatment.…”

“…The hybridised membrane was exposed to an X-ray film in an intensifying cassette under dark room conditions. The cassette was placed at –80 °C for 3 d and developed to visualise the bands 65 .…”

“…To analyse the salinity tolerance at germination level, seeds of EcbZIP17 transgenic plants and WT were germinated on MS agar medium supplemented with 250 mM NaCl 2 and to analyse salt tolerance at mature plant level, 30-d-old transgenic and WT plants were saturated in 200 mM NaCl solution at 3-d intervals continuously for one month. To evaluate response to drought stress at mature plant level, transgenic and WT plants were subjected to water withdrawal for 11 d. Further for recovery study from drought stress, the plants were saturated with water 65 . For the heat stress treatment, 20-d old transgenic plants were grown on MS medium without kanamycin at 42 °C for 3d.…”

GhDRIN1, a novel drought-induced gene of upland cotton (Gossypium hirsutum L.) confers abiotic and biotic stress tolerance in transgenic tobacco

“…Petit Havana) was used. The plant culture conditions and related parameters under in vitro and transgenic glass house conditions were as reported earlier (Dhandapani et al 2014).…”

“…The blot was hybridized at 42°C with a [ 32 P]-dCTP labeled 390-bp GhDRIN1 fragment using with mega-prime DNA labeling system (Amersham Biosciences). The complete procedure for Southern and northern blot analysis was followed as described earlier (Dhandapani et al 2014).…”