A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry

Abad‐García, Beatriz; Berrueta, Luis Á.; Garmón-Lobato, Sergio; Gallo, Blanca; Vicente, Francisca

doi:10.1016/j.chroma.2009.05.039

Cited by 224 publications

(194 citation statements)

References 32 publications

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“…Compounds 1-7 and 9 were found to be resistant to acid hydrolysis. The on line UV-visible spectra of all flavonoids from P. incarnata and peaks at 20.1 (compound 9) and 22.2 min (compound 2) from S. guianensis exhibited band I (330-350 nm) and band II (254-272 nm) with similar intensities, typical of flavones (Abad-Garcia et al, 2009).…”

Section: Resultsmentioning

confidence: 97%

“…The retention time on the column is governed not only by the polarity of the molecules but also by their size. The sugar position is more important for the retention time than the nature of sugar (Abad-Garcia et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, it is very important to obtain information that permit to differentiate glycosylation positions, localize interglycosidic linkages in glycan moieties and evaluate structures of aglycone and sugar rings (Abad-Garcia et al, 2009). The hydroethanolic extract of Siparuna guianensis Aubl., Siparunaceae, have an acid pH (5.0).…”

Section: Resultsmentioning

confidence: 99%

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Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Negri¹,

Santi²,

Tabach³

2012

Rev. bras. farmacogn.

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Siparuna guianensis Aubl., Siparunaceae, is used as anxiolytic plants in folk medicine by South-American indians, "caboclos" and river-dwellers. This work focused the evaluation of phenolic composition of hydroethanolic extract of S. guianensis through HPLC-DAD-ESI/MS/MS. The constituents exhibited protonated, deprotonated and sodiated molecules and the MS/MS fragmentation of protonated, deprotonated and sodiated molecules provided product ions with rich structural information. Vicenin-2 (apigenin-6,8-di-C-glucoside) was the main constituent found in S. guianensis together quercetin-3,7-di-O-rhamnoside and kaempferol-3,7-di-O-rhamnoside. A commercial extract of Passiflora incarnata (Phytomedicine) was used as surrogate standard and also was analyzed through HPLC-DAD-ESI/ MS/MS, showing flavones C-glycosides as constituents, among them, vicenin-2 and vitexin. The main constituent was vitexin. Flavonols triglycosides were also found in low content in S. guianensis and were tentatively characterized as quercetin-3-O-rutinoside-7-O-rhamnoside, quercetin-3-O-pentosyl-pentoside-7-O-rhamnoside and kaempferol-3-O-pentosyl-pentoside-7-O-rhamnoside. Apigenin and kaempferol derivatives had been reported as anxiolytic agents. Flavonoids present in this extract were correlated with flavonoids reported as anxiolytics.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

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Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Negri¹,

Santi²,

Tabach³

2012

Rev. bras. farmacogn.

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“…The hyphenated technique of HPLC coupled with photo diode array (PDA) spectrophotometers and soft electrospray ionization (ESI) with tandem mass spectrometry is the approach most recently used for the characterization of polyphenolic fruits profile [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Blood Orange Anthocyanins in Fruit Beverages: How the Commercial Shelf Life Reflects the Quality Parameter

et al. 2015

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Pigmented oranges are a high value typical product, used for the production of healthy juice-based drinks due to the peculiar presence of anthocyanins as dietary antioxidants. A qualitative HPLC/PDA/MS analytical method for verifying presence/absence of blood orange anthocyanins was validated in accordance with Eurachem guide in terms of accordance (100%), specificity/selectivity, repeatability of retention time (CV < 0.5%), limit of detection (0.36 mg/L) and limit of decision (0.41 mg/L). Fifty commercial red orange juice beverages were collected on the market and analyzed to assess the occurrence of blood orange anthocyanins. The results showed that only 60% of analyzed products have a minimum content of anthocyanins of blood orange, guaranteeing the specifications of the product they publicize and sell until the expiration date. Therefore, it is possible to envisage a gradual degradation of the specific parameter (anthocyanins) or willful misconduct of producers (non-use of blood orange juice). In both cases, the consumer buys a product with high added value without such value being guaranteed. This information will be useful to revise the expiration date of these products, the percentage of blood orange juice used in the preparation and/or the storage conditions of the products. OPEN ACCESSBeverages 2015, 1 83

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“…Furthermore, under high collision energy conditions, characteristic fragmentation of the anthocyanidin base occurs which provides additional information on the substituents of the aglycone. [44,93]. Notation: Dp: delphinidin, Cy:cyanidin, Pt: petunidin, Pe: peonidin, and Mv: malvidin.…”

Section: Detection Methodsmentioning

confidence: 99%

Comprehensive two-dimensional liquid chromatographic analysis of anthocyanins

Willemse¹,

Stander²,

Tredoux³

et al. 2014

Journal of Chromatography A

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A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry

Cited by 224 publications

References 32 publications

Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Blood Orange Anthocyanins in Fruit Beverages: How the Commercial Shelf Life Reflects the Quality Parameter

Comprehensive two-dimensional liquid chromatographic analysis of anthocyanins

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