2014
DOI: 10.4161/mabs.29680
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A general approach to antibody thermostabilization

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Cited by 48 publications
(53 citation statements)
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“…25,26,42 Our methods can therefore potentially streamline the huge amount of effort put into the discovery process. [20][21][22]27,28 Our results support the utility of next-generation sequencing in combinatorial library screening. 10,29,32 Hyperimmunization leads to a high degree of polarization of the B-cell repertoire in a particular compartment, such that the dominant antibodies are highly represented and combinatorial screening by a technique with low expression bias, such as yeast display, can further yield high-affinity native antibodies.…”
Section: Discussionsupporting
confidence: 69%
See 1 more Smart Citation
“…25,26,42 Our methods can therefore potentially streamline the huge amount of effort put into the discovery process. [20][21][22]27,28 Our results support the utility of next-generation sequencing in combinatorial library screening. 10,29,32 Hyperimmunization leads to a high degree of polarization of the B-cell repertoire in a particular compartment, such that the dominant antibodies are highly represented and combinatorial screening by a technique with low expression bias, such as yeast display, can further yield high-affinity native antibodies.…”
Section: Discussionsupporting
confidence: 69%
“…[21][22][23]27,28 Our lab has pioneered methods for the discovery of high affinity antigen-specific antibodies directly via mining of the immunoglobulin repertoire by capitalizing on next-generation sequencing technologies without the need for screening. [29][30][31][32] Specifically, we developed methods for high-throughput determination of the natively paired V H :V L repertoire from single B cells.…”
Section: Introductionmentioning
confidence: 99%
“…Our directed evolution approach used for isolating the P4 V H domain by co-selecting affinity and stability mutations17 shares similarities with previous studies aimed at using natural antibody diversity and various display methods to optimize antibody affinity and/or stability264243444546474849. For example, our approach of grafting peptides into CDR3 and selecting for sets of mutations that contribute to affinity and stability using yeast surface display shares some commonalities with approaches in which CDRs are grafted onto stability-engineered antibody scaffolds and mutations are selected that optimize affinity and stability using display methods264243.…”
Section: Discussionmentioning
confidence: 82%
“…Thermostabilized Abs have been developed as robust affinity reagents for refrigeration-free use 45,46,39 . Thus, we examined whether a high T m Ab would be advantageous in preventing the unfolding of its cognate antigen as temperature was varied.…”
Section: Resultsmentioning
confidence: 99%