A Genetic and Cytogenetic Map for the Duck (Anas platyrhynchos)

Abstract

A genetic linkage map for the duck (Anas platyrhynchos) was developed within a cross between two extreme Peking duck lines by linkage analysis of 155 polymorphic microsatellite markers, including 84 novel markers reported in this study. A total of 115 microsatellite markers were placed into 19 linkage groups. The sexaveraged map spans 1353.3 cM, with an average interval distance of 15.04 cM. The male map covers 1415 cM, whereas the female map covers only 1387.6 cM. All of the flanking sequences of the 155 polymorphic loci-44 monomorphic loci and a further 41 reported microsatellite loci for duck-were blasted against the chicken genomic sequence, and corresponding orthologs were found for 49. To integrate the genetic and cytogenetic map of the duck genome, 28 BAC clones were screened from a chicken BAC library using the specific PCR primers and localized to duck chromosomes by FISH, respectively. Of 28 BAC clones, 24 were detected definitely on duck chromosomes. Thus, 11 of 19 linkage groups were localized to 10 duck chromosomes. This genetic and cytogenetic map will be helpful for the mapping QTL in duck for breeding applications and for conducting genomic comparisons between chicken and duck.

Successful Identification of Duck Genome Region Determining Desirable Uniformity of Meat Performance Traits

“…The primers for the amplification of microsatellite sequences were chosen based on the (Huang et al, 2006). Finally, 29 polymorphic microsatellite markers were further analyzed.…”

“…Therefore, the analysis of the association between Anas platyrhynchos the genome and phenotypes is still limited. However, over the last decades a number of studies on the associations of single loci with performance traits of ducks (Huang et al, 2005(Huang et al, , 2006Maak et al, 2000;Wu et al, 2008;Gong et al, 2014;Mucha et al, 2014b), using different methodological approaches, have been published. Generally, they focus on single measures per individual.…”

Extensive linkage disequilibrium in a wild bird population

“…[1][2][3]). However, genomic resources for the majority of freeliving vertebrates of ecological and evolutionary importance are still scarce.…”

Optimization of Volumetric Computed Tomography for Skeletal Analysis of Model Genetic Organisms

“…In confirmation of the duck's importance as an emerging genetic model organism, a genomic linkage map recently generated from the cross of two Pekin ducks was published to facilitate genomic comparisons between chickens and ducks (Huang et al, 2006).…”