A genetic cluster of OXA-244 carbapenemase-producing <i>Escherichia coli</i> ST38 with putative uropathogenicity factors in the Netherlands

Notermans, Daan W.; Schoffelen, Annelot F.; Landman, Fabian; Wielders, Cornelia C H; Witteveen, Sandra; Ganesh, Varisha A; Santen-Verheuvel, Marga van; Greeff, Sabine C. de; Kuijper, Ed J.; Hendrickx, Antoni P. A.; Wintermans, B.B.; Hall, Michael; Bijllaardt, Wouter van den; Spijkerman, Ingrid J. B.; Dijk, Karin van; Zwart, B.; Diederen, Bram M. W.; Voß, Andreas; Dorigo-Zetsma, J. W.; Ott, Alewijn; Oudbier, J. H.; Vusse, M. van der; Vlek, A. L. M.; Buiting, A. G. M.; Bode, L.; Paltansing, Sunita; Griethuysen, A. J. van; Reijer, Martijn den; Trijp, M. J. C. A. van; Burgel, N.D. van; Muller, Anouk E.; Linden, M. P. M. van der; Rijn, M. van; Wolfhagen, M. J. H. M.; Waar, Karola; Kolwijck, Eva; Silvis, Welmoed; Schülin, T.; Damen, Marjolein; Dinant, Sander; Mens, Suzan P. van; Melles∗, Damian C.; Stuart, J. W. T. Cohen; Ogtrop, M L van; Jansz, A R; Dam, Alje P. van; Wertheim, H.F.L.; Maraha, B.; Sinnige, Jan C.; Mattsson, Eva; Mascini, E M; Stam, A.; Jong, E. de; Roescher, Nienke; Heikens, E.; Steingrover, Radjin; Troelstra, Annet; Bathoorn, Erik; Trienekens, T. A. M.; Dam, D W van; Brauwer, E.I.G.B. De; Stals, Frans

doi:10.1093/jac/dkac307

Cited by 8 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expansion of OXA-244-Ec ST38 has been one of the most worrying phenomena in recent epidemiology of CPE in Europe, addressed in two consecutive ECDC RRAs in February 2020 and July 2021 [11] and in several reports from different countries [12][13][14][15][16]. This concern has arisen from the significance of E. coli as a major human pathogen which causes community-acquired infections [1] and from the role of carbapenems as the last line drugs in the treatment of severe MDR Gramnegative infections [3].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Large hospital outbreak caused by OXA-244-producing Escherichia coli sequence type 38, Poland, 2023

Izdebski,

Biedrzycka,

Urbanowicz

et al. 2024

Eurosurveillance

View full text Add to dashboard Cite

In February 2023, Escherichia coli sequence type (ST) 38 producing oxacillinase 244 (OXA-244-Ec ST38) was detected from three patients in a hospital in western Poland. Overall, OXA-244-Ec ST38 was detected from 38 colonised patients in 13 wards between February and June 2023. The outbreak was investigated on site by an infection control team, and the bacterial isolates were characterised microbiologically and by whole genome sequencing. We could not identify the primary source of the outbreak or reconstruct the transmission sequence. In some of the 13 affected wards or their groups linked by the patients’ movement, local outbreaks occurred. The tested outbreak isolates were resistant to β-lactams (penicillins, cephalosporins, aztreonam and ertapenem) and to trimethoprim-sulfamethoxazole. Consistently, apart from bla OXA-244, all isolates contained also the bla CMY-2 and bla CTX-M-14 genes, coding for an AmpC-like cephalosporinase and extended-spectrum β-lactamase, respectively, and genes conferring resistance to trimethoprim-sulfamethoxazole, sul2 and dfrA1. Genomes of the isolates formed a tight cluster, not of the major recent European Cluster A but of the older Cluster B, with related isolates identified in Germany. This outbreak clearly demonstrates that OXA-244-Ec ST38 has a potential to cause hospital outbreaks which are difficult to detect, investigate and control.

show abstract

Section: Discussionmentioning

confidence: 99%

“…This sub-lineage, notably homogeneous and co-expressing the cefotaximase (CTX)-M-27 ESBL was first identified in 2016. Another sub-lineage, Cluster B, detected in 2013, is less frequent, more diversified genetically and correlates with the CTX-M-14 enzyme [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Large hospital outbreak caused by OXA-244-producing Escherichia coli sequence type 38, Poland, 2023

Izdebski,

Biedrzycka,

Urbanowicz

et al. 2024

Eurosurveillance

View full text Add to dashboard Cite

show abstract

“…A variety of PUFs, associated with UPEC, were detected in cluster isolates ( Appendix 2 Table 5, Appendix 1 Figure, panel E), including adhesins (e.g., sfaH , pili papGII / papGIII ), toxins (e.g., α-hemolysin, cytotoxic necrotizing factor-1, and E. coli uropathogenic-specific protein) ( 4 , 7 , 8 ). Cluster isolates carried significantly more (mean 18) PUFs, than the other E. coli isolates from CPE surveillance (mean nonurine isolates, 7; urine isolates, 9; previously reported OXA-244 E. coli ST38 isolates, 8; p<0.001 by Mann-Whitney U-test) ( Appendix 1 Figure, panel F) ( 1 ). We identified additional uropathogenicity determinants curli, type-I fimbriae, S-fimbriae, flagella, and group 2 capsule genes but not group 3 capsule genes.…”

mentioning

confidence: 82%

“…We recently described OXA-244 carbapenemase-producing Escherichia coli sequence type (ST) 38 with putative uropathogenicity factors ( 1 ). Here we report a genetic cluster of 20 OXA-48–producing uropathogenic Escherichia coli (UPEC) ST127 isolates in the Netherlands.…”

mentioning

confidence: 99%

“…For all isolates, we perform meropenem Etest, carbapenem inactivation method, next-generation sequencing (NGS; lllumina, https://www.illumina.com ), and long-read sequencing (Oxford Nanopore Technologies, https://www.nanoporetech.com ). We use NGS data to analyze the Clermont phylotype ( 2 ), core-genome single-nucleotide polymorphisms, classical multilocus sequence typing (MLST) STs, and in-house E. coli whole-genome MLST (wgMLST) types ( 1 , 3 ). We also evaluated presence of antimicrobial resistance genes (AMRfinder, https://www.ncbi.nlm.nih.gov/pathogens/antimicrobial-resistance/AMRFinder ), plasmid replicons (PlasmidFinder, https://cge.food.dtu.dk/services/PlasmidFinder ), and 31 previously described putative uropathogenicity factors (PUFs) using an in-house PUFfinder ( 4 ).…”

mentioning

confidence: 99%

See 1 more Smart Citation

OXA-48–Producing Uropathogenic Escherichia coli Sequence Type 127, the Netherlands, 2015–2022

Mulder,

Notermans,

Wielders

et al. 2023

Emerg. Infect. Dis.

Self Cite

View full text Add to dashboard Cite

During 2015–2022, a genetic cluster of OXA-48–producing uropathogenic Escherichia coli sequence type 127 spread throughout the Netherlands. The 20 isolates we investigated originated mainly from urine, belonged to Clermont phylotype B2, and carried 18 genes encoding putative uropathogenicity factors. The isolates were susceptible to first-choice antimicrobial drugs for urinary tract infections.

show abstract

Several epidemic and multiple sporadic genotypes of OXA-244-producing Escherichia coli in Poland; predominance of the ST38 clone

Biedrzycka,

Izdebski,

Gniadkowski

et al. 2024

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

OXA-244-producing Escherichia coli (OXA-244-Ec) has disseminated in Europe, mostly in the community. In Poland it has spread since 2017, especially in 2023, but in contrast to other countries, all isolates have been identified in hospitals so far. The isolates (n = 101) represented one large and two limited outbreaks in different regions, and multiple epidemiologically and genetically non-related organisms. The OXA-244-Ec population consisted of 14 STs, with ST38 dominating. The ST38 isolates belonged to two major lineages, Clusters A and B, responsible for two of the hospital outbreaks. Enhanced concern and vigilance are necessary in the OXA-244-Ec surveillance.

show abstract

A genetic cluster of OXA-244 carbapenemase-producing Escherichia coli ST38 with putative uropathogenicity factors in the Netherlands

Abstract: Emergence of urine-associated Escherichia coli ST38 producing OXA-244 harbouring putative uropathogenicity factors in the Netherlands.

Cited by 8 publications

References 10 publications

Large hospital outbreak caused by OXA-244-producing Escherichia coli sequence type 38, Poland, 2023

Large hospital outbreak caused by OXA-244-producing Escherichia coli sequence type 38, Poland, 2023

OXA-48–Producing Uropathogenic Escherichia coli Sequence Type 127, the Netherlands, 2015–2022

Several epidemic and multiple sporadic genotypes of OXA-244-producing Escherichia coli in Poland; predominance of the ST38 clone

Contact Info

Product

Resources

About