A genetic linkage map for <i>Tribolium confusum</i> based on random amplified polymorphic DNAs and recombinant inbred lines

Yezerski, Ann; Stevens, Lori; Ametrano, J.

doi:10.1046/j.1365-2583.2003.00438.x

Cited by 5 publications

(3 citation statements)

References 67 publications

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“…It is unknown which specific variables led to the generally low infection levels in this study, although the choice of using one particular recombinant inbred line may have contributed. A repeat of the study using several inbred lines (such as recombinant inbred lines we created previously (Yezerski et al ., 2003)) could further elucidate relationships amongst the variables as well as the genetic components.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of multiple variables on the levels of infection of the rat tapeworm,Hymenolepis diminuta, in its intermediate host, the confused flour beetleTribolium confusum

2020

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The interaction of the rat tapeworm, Hymenolepis diminuta, with its intermediate beetle host, Tribolium confusum, is a well-studied model system. However, there is so much variation in the methods and designs of the studies that it is difficult to draw comparisons. This study simultaneously compared several aspects of the infection protocol including beetle age, sex, density and mating status; parasite egg condition, infection environment humidity and the times for the three steps of infection: starvation, feeding and post-feeding development. Although statistically limited by low prevalence and intensity levels, we were able to detect the relative effects of the variables. The effects of these variables on prevalence (percent infection) and intensity (mean number of cysticercoids) do not necessarily correlate with each other. Egg condition, reduced starvation times, higher beetle density and longer development times reduced prevalence. However, differences in intensity were only detected with older beetles. When coupled with survivorship data, our study found that our current infection protocol is optimal for infection success. However, the results suggest extending the study to other intermediate hosts and the inclusion of additional variables.

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Section: Discussionmentioning

confidence: 99%

Comparison of the effects of multiple variables on the levels of infection of the rat tapeworm,Hymenolepis diminuta, in its intermediate host, the confused flour beetleTribolium confusum

2020

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“…In insects, RAPD and AFLP markers have been extensively used to generate genetic maps. RAPD‐based linkage maps have been constructed for genomes of honeybee (Hunt & Page 1995), silkworm (Yasukochi 1998), beetle (Beeman & Brown 1999; Yezerski et al . 2003) and sawfly (Nishimori et al .…”

Section: Major Applications Of Molecular Markers In Studying Insect Ementioning

confidence: 99%

Molecular marker systems in insects: current trends and future avenues

2006

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Insects comprise the largest species composition in the entire animal kingdom and possess a vast undiscovered genetic diversity and gene pool that can be better explored using molecular marker techniques. Current trends of application of DNA marker techniques in diverse domains of insect ecological studies show that mitochondrial DNA (mtDNA), microsatellites, random amplified polymorphic DNA (RAPD), expressed sequence tags (EST) and amplified fragment length polymorphism (AFLP) markers have contributed significantly for progresses towards understanding genetic basis of insect diversity and for mapping medically and agriculturally important genes and quantitative trait loci in insect pests. Apart from these popular marker systems, other novel approaches including transposon display, sequence-specific amplification polymorphism (S-SAP), repeat-associated polymerase chain reaction (PCR) markers have been identified as alternate marker systems in insect studies. Besides, whole genome microarray and single nucleotide polymorphism (SNP) assays are becoming more popular to screen genome-wide polymorphisms in fast and cost effective manner. However, use of such methodologies has not gained widespread popularity in entomological studies. The current study highlights the recent trends of applications of molecular markers in insect studies and explores the technological advancements in molecular marker tools and modern high throughput genotyping methodologies that may be applied in entomological researches for better understanding of insect ecology at molecular level.

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“…Two of these were from stocks containing 100 g of flour kept at 29°C and contained either beetles from the b‐IV or b‐Pakistan strains. Two other T. confusum samples came from vials containing about 10 g of flour kept at room temperature that contained beetles from one of two randomly chosen recombinant inbred (RI) lines (see Yezerski et al. 2003 for construction of RI lines).…”

Section: Source Of Bacterial Culturesmentioning

confidence: 99%

The effects of the presence of stored product pests on the microfauna of a flour community

et al. 2005

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Aims: To determine the effect that the presence of some beetles have on the species of bacteria found in their flour. Methods and Results: Bacteria were isolated from flour that either did not contain beetles, contained Tribolium beetles in different environments, or contained either Stegobium paniceum or Lasioderma serricorne. These bacteria were tentatively identified by both the gas chromatography-fatty acid methyl esters (GC-FAME) method and partial sequencing of the 16S rRNA gene. All samples contained Bacillus species including the controls, but the nonTribolium beetles and a Tribolium beetle line known to have low benzoquinones also contained Enterococcus and Enterobacter species. Additionally an unidentified bacteria isolate in the Enterobacteriaceae was also found in the L. serricorne sample. Our results also suggest incongruent identifications when using the GC-FAME method vs sequencing. Conclusions: Certain species of bacteria can be introduced by the presence of insect pests, but the diversity of species is far less in stocks of Tribolium beetles. Significance and Impact of the Study: Stored product pests can alter the bacterial community. Isolated species from this study show a strong genetic relationship to each other, suggesting an isolated evolving system. A unique bacteria was also isolated. GC and sequencing methods of identification are compared.

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A genetic linkage map for Tribolium confusum based on random amplified polymorphic DNAs and recombinant inbred lines

Cited by 5 publications

References 67 publications

Comparison of the effects of multiple variables on the levels of infection of the rat tapeworm,Hymenolepis diminuta, in its intermediate host, the confused flour beetleTribolium confusum

Comparison of the effects of multiple variables on the levels of infection of the rat tapeworm,Hymenolepis diminuta, in its intermediate host, the confused flour beetleTribolium confusum

Molecular marker systems in insects: current trends and future avenues

The effects of the presence of stored product pests on the microfauna of a flour community

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