A genetic method for sex determination in Ovis spp. by interruption of the zinc finger protein, Y-linked (ZFY) gene on the Y chromosome

Zhang, Yong Sheng; Du, Ying Chun; Sun, Li Rong; Wang, Xu Hai; Liu, Shuai Bing; Xi, Ji Feng; Li, Chao Cheng; Ying, Rui; Song, Jukun; Wang, Xiang Zu; Hong, Shaodong; Jia, Bin

doi:10.1071/rd17339

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…A further strategy is based on the injection of testis with shRNA vectors, which was performed to target the zinc finger protein Y-linked (ZFY) gene. This strategy was applied as a genetic method of sex control and to bias the sex ratio toward females in sheep (Zhang et al, 2018b). In general, RNAi-based approaches are an interesting tool for mediating loss of gene expression by targeting the products of gene transcription (mRNA).…”

Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning

confidence: 99%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important model animals in agricultural, pharmaceutical, and biomedical research. Genome engineering has been widely applied to sheep and goat research. Pronuclear injection and somatic cell nuclear transfer represent the two primary procedures for the generation of genetically modified sheep and goats. Further assisted tools have emerged to enhance the efficiency of genetic modification and to simplify the generation of genetically modified founders. These tools include sperm-mediated gene transfer, viral vectors, RNA interference, recombinases, transposons, and endonucleases. Of these tools, the four classes of site-specific endonucleases (meganucleases, ZFNs, TALENs, and CRISPRs) have attracted wide attention due to their DNA double-strand break-inducing role, which enable desired DNA modifications based on the stimulation of native cellular DNA repair mechanisms. Currently, CRISPR systems dominate the field of genome editing. Gene-edited sheep and goats, generated using these tools, provide valuable models for investigations on gene functions, improving animal breeding, producing pharmaceuticals in milk, improving animal disease resistance, recapitulating human diseases, and providing hosts for the growth of human organs. In addition, more promising derivative tools of CRISPR systems have emerged such as base editors which enable the induction of single-base alterations without any requirements for homology-directed repair or DNA donor. These precise editors are helpful for revealing desirable phenotypes and correcting genetic diseases controlled by single bases. This review highlights the advances of genome engineering in sheep and goats over the past four decades with particular emphasis on the application of CRISPR/Cas9 systems.

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Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning

confidence: 99%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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“…The formation of X or Y sperm during spermatogenesis plays an important role in sex determination of offspring [19]. Previous studies in our laboratory found that Zfx or Zfy siRNA can change the sex ratio of animal offspring, such as cows, mouse, sheep, swine [14,17,20]. Cervus elaphus is a special economic animal and its antler has great market economic value.…”

Section: Discussionmentioning

confidence: 95%

“…At present, there are only a little research on Zfx gene silencing to make the sex shift of offspring, so the mechanism of action of Zfx in sperm is not clear. Some studies have shown that reducing the level of Zfx/Zfy mRNA expression will make the physiological function of X/Y sperm worse, which can influence offspring gender [14,17,[20][21][22]. Zfy is closely related to sperm head and tail formation and neck development [23], which is highly expressed between meiosis I and II.…”

Section: Discussionmentioning

confidence: 99%

The RNAi vecter construction and verification of the functions in sexual control of Cervus elaphus Zfx gene

Wei

Song

et al. 2019

Preprint

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Background: Zinc finger protein X-linked (Zfx) was regarded to be a sex determination factor, and plays a critical role in spermatogenesis. RNAi is an effective method of silencing Zfx/Zfy mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of Cervus elaphus. The objective of this study was first to explore an efficient method to alter the Cervus elaphus offspring sex-ratio by silencing the genes Zfx during spermatogenesis.Results: Three recombinant expression vectors pLL3.7/A, pLL3.7/B and pLL3.7/C were constructed to interrupt the Zfx gene. The results showed that the expression of Zfx mRNA was significantly silenced by pLL3.7/A (P < 0.01), compared with the control group. The group injected with pLL3.7/A produced 94 Cervus elaphus, including 68 males and 26 females. The male rates (72.34%) were significantly higher than the control groups (P < 0.01).Conclusions: Our experiment suggest that the Zfx gene plays a significant role in the process of X-sperm formation. Zfx siRNA may be a useful approach to control offspring sex in Cervus elaphus.

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“…2015 ). Sheep with interrupted ZFY showed abnormal sperm morphology and biased sex ratio in offspring ( Zhang et al. 2018 ), and Zfy1 and Zfy2 double knockout mice were infertile ( Nakasuji et al.…”

Section: Discussionmentioning

confidence: 99%

Deep sequencing of fosmid clones indicates gene conversion in the male-specific region of the giant panda Y chromosome

Han

Guang

Wan

et al. 2018

Genome Biology and Evolution

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The giant panda (Ailuropoda melanoleuca) is popular around the world and is widely recognized as a symbol of nature conservation. A draft genome of the giant panda is now available, but its Y chromosome has not been sequenced. Y chromosome data are necessary for study of sex chromosome evolution, male development, and spermatogenesis. Thus, in the present study, we sequenced two parts of the giant panda Y chromosome utilizing a male giant panda fosmid library. The sequencing data were assembled into two contigs, each ∼100 kb in length with no gaps, providing high-quality resources for studying the giant panda Y chromosome. Annotation and transposable element comparison indicates varied evolutionary pressure in different regions of the Y chromosome. Two genes, zinc finger protein, Y-linked (ZFY) and lysine demethylase 5D (KDM5D), were annotated and gene conversion was observed for ZFY exon 7. Phylogenetic analysis also revealed that this gene conversion event happened independently in multiple mammalian lineages, indicating a putative mechanism to maintain the function of this particular gene on the Y chromosome. Furthermore, a transposition event, discovered through comparative alignment with the giant panda X chromosome sequence, may be involved in the process of gaining new genes on the Y chromosome. Thus, these newly obtained Y chromosome sequences provide valuable insights into the genomic patterns of the giant panda.

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A genetic method for sex determination in Ovis spp. by interruption of the zinc finger protein, Y-linked (ZFY) gene on the Y chromosome

Cited by 9 publications

References 37 publications

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

The RNAi vecter construction and verification of the functions in sexual control of Cervus elaphus Zfx gene

Deep sequencing of fosmid clones indicates gene conversion in the male-specific region of the giant panda Y chromosome

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