A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au

Abstract

Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.

A Review on the Development of Gold and Silver Nanoparticles-Based Biosensor as a Detection Strategy of Emerging and Pathogenic RNA Virus

“…Another study that implemented the mechanism of fluorescence quenching was observed through the application of carbon dots (CDs) and iron magnetic nanoparticle-capped Au (Fe 3 O 4 @Au) [87]. In the study, Fe 3 O 4 @Au was used to adsorb the CDs on its surface.…”

Comparison between various biosensor methods for human T-lymphotropic virus-1 (HTLV-1) detection

“…Ghobadi et al employed the photoluminescence method to immobilize the HTLV-1 Tax gene on the platform modified using carbon dots and Fe 3 O 4 -capped Au. The detection limit of HTLV-1 Tax DNA was found at 10 nM with a linear range of 10-320 nM [31]. In parallel work, this group fabricated a new platform to detect the 122-base segment of HTLV-1 Tax gene based on the graphene oxide (GO) and gold nanoparticles (AuNPs).…”

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

“…The detection limit of 15 fM and 5 fM were acquired from an ssDNA-CD-AuNPs complex and ssDNA-CD-AuNPs/GO assembly, respectively. Zarei-Ghobadi et al introduced a genosensor through quenching the CDs fluorescence in the attendance of Fe 3 O 4 -capped Au (Fe 3 O 4 @Au) for diagnosis of a special region of HTLV-1 genome [69]. Two dedicated probes were designed to diagnosis target DNA.…”

“…The higher photocurrent response of HTLV-1 DNA was observed than those of mismatch DNA, which confirmed that the constructed biosensor could efficiently discern various DNA sequences. The fabricated photoelectrochemical biosensor showed an ultrasensitive diagnosis of HTLV-1 [ 60] FRET between CDs and Fe 3 O 4 @Au NPs 10 nM 10-320 nM [69] CdTe QDs tagged complementary DNA probe 10 pg/ml 10 pg/ml -21 ng/ml [70] Hairpin shaped probe tagged with AG NCs 8.5 nM 10-200 nM [75] Photoelectrochemical Cascade photoactive materials and triple helix molecular switch 0.65 fM 1 fM -1 nM [115] DNA (11.3 aM). A PEC biosensor was fabricated by Wang et.al.…”