A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells

Abstract

A lectin exhibiting antiproliferative activity on tumor cell lines but devoid of antifungal activity has been purified from Phaseolus vulgaris cv. Green Dragon no. 8 seeds. The lectin was a 60 kDa dimeric protein with two 30 kDa subunits. It was a glucosamine-specific lectin as implied from the inhibitory effect of glucosamine on hemagglutinating activity of the lectin. The steps for isolation of the lectin involved Affi-gel blue gel (affinity gel), Mono Q (anion exchanger), and Superdex 75 column (size exclusion). The lectin was purified 20.8-fold from the crude extract of the beans. The purified lectin showed antiproliferative activity on breast cancer MCF7 cell line and nasopharyngeal cancer HONE1 and CNE2 cell lines, but a low activity on normal skin fibroblast HSF98 cell line. The lectin was shown to induce apoptosis on HONE1 cells, as indicated by increased phosphatidylserine externalization and mitochondrial depolarization. It also blocked HONE1 cell division and kept the cells at the G2/M phase of the cell cycle.

Leguminosae Lectins as Biological Tools in Medical Research: a Review

“…A lectin from Phaseolus vulgaris L. 'French bean number 35' induce apoptosis in MCF-7 cells [91]; and a polygalacturonic acid-specific lectin, named BTKL, isolated from seeds of Phaseolus vulgaris L. "Blue tiger king", exerted a potent antiproliferative action on Hep G2 cells [92]; and Phaseolus vulgaris L. 'Green Dragon number 8' beans contain a glucosamine-binding lectin that could inhibit nasopharyngeal carcinoma HONE1 cells after treatment for 24 h (IC50 = 62.3 µM), 48 h (IC50 = 31.6 µM), and 72 h (IC50 = 28.7 µM), preventing the cells from dividing and kept them at the G2/M phase. It also had an effect on nasopharyngeal carcinoma CNE2 cells, after 24 and 48 h treatment [93]. The EAPL showed antitumor activity against nasopharyngeal carcinoma (CNE-1, CNE-2, HNE-2) cell lines, breast cancer cells (MCF-7) e hepatocellular carcinoma (HepG2), inhibiting their proliferation in a dose dependent manner.…”

Optimalisasi Sayuran Buncis Sebagai Upaya Peningkatan Perekonomian Keluarga Di Desa Meunasah Kulam

“…Hasil penelitian lainnya menyatakan bahwa buncis dapat diolah sebagai obat antidiabetes dikarenakan buncis kaya kandungan senyawa bioaktif yang mampu menghidrolisis enzim sehingga protein membentuk senyawa peptida bioaktif yang mengandung antioksidan yang yang tinggi sehingga usus tidak banyak menyerap glukosa dan merangsang sekresi insulin (de Souza Rocha et al, 2015;Maripa et al, 2019). Sayuran buncis dapat digunakan sebagai obat antikanker karena mengandung senyawa glucosamine (Chan et al, 2015).…”