A GLUTs/GSH cascade targeting-responsive bioprobe for the detection of circulating tumor cells

Abstract

A GLUTs/GSH cascade targeting-responsive bioprobe, GluCC, was rationally designed and synthesized for the first time via the coordination of copper ions with glucose modified coumarin derivative ligand (GluC). GluCC can...

Detection of S2− in Water by a Glucose Enhanced Water-Soluble Fluorescent Bioprobe

“…in an aqueous solution was 0.018 and 0.104. What is more, according to our previous study [ 22 ] and a Job’s plot ( Figure S12 ), GluC and Cu 2+ have formed the 1:1 complex. Besides, a peak of Cu[GluC] was found at 834.2602 in the mass spectrum ( Figure S10 ), which is corresponding to [GluC + Cu 2+ ].…”

“…Therefore, the fluorescence stability of Cu[GluC] and GluC were studied in water with different pHs. According to our previous study [ 22 ], both Cu[GluC] and GluC displayed excellent stability in the pH range of 6–11, which is better than other S 2− fluorescent probes [ 23 , 28 , 30 , 31 ]. This result indicates that Cu[GluC] is suitable for the S 2− detection in water with a wide pH range from 6 to 11.…”

“…Thus, all the above results suggest that the binding ratio of GluC and Cu 2+ is 1:1. The K d for Cu[GluC] was calculated to be 2.033 μM in our previous study [ 22 ], which indicates the good stability of Cu[GluC].…”

“…Finally, GluC reacted with Cu(ClO 4 ) 2 ·6H 2 O to obtain Cu[GluC]. The detailed synthesis route was provided in Supplementary Material according to our previous study [ 22 ] with some changes. 1 H NMR and HRMS of the synthesized compounds were provided in Figures S1–S10 .…”

“…The excitation is carried out at 450 nm, and the slit width of emission and excitation is 5 nm. Besides, the calculation of quantum yield (Φ), binding constant ( K α ), dissociation constant ( K d ) in the experiment refers to the methods provided by our previous study [ 22 ]. Limit of detection (LOD) and limit of quantification (LOQ) are calculated as follows: “LOD = 3 σ/k”, “LOQ = 10 σ/k”, where σ is the standard deviation of blank and k is the linear correlation slope of fluorescence intensity at 494 nm relative to the concentrations of S 2− .…”