A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Abstract

Sirolimus, is a potent macrolide immunosuppressant, with a long half-life, narrow therapeutic window, and large individual variations in pharmacokinetics. In this study, an indirect enzyme-linked immunosorbent assay (ic-ELISA) and a...

A paper sensor for unbound valproic acid detection in human serum

“…The GNP-labeled mAbs were prepared as described in a previous report. 38 After adding 0.1 M potassium carbonate dropwise to adjust the pH to 8–9, 10 mL of the GNP solution was mixed with mAb 3G10 and allowed to stand at RT for 1 h. Electrostatic interactions occurred between the GNPs and mAbs. Next, 10% BSA (5 mL) was added to the solution and allowed to stand at RT for 2 h to achieve the purpose of blocking GNPs.…”

A gold-based immunochromatographic strip for the specific detection of tacrolimus in whole blood

“…As described in previous reports with some modifications, the hydroxyl and carbon groups of tacrolimus were reacted with succinic anhydride and CMO, respectively. 37,38 The resulting tacrolimus derivatives were named as tacrolimus-1 and tacrolimus-2, with carboxyl groups at different positions. The derivation procedures of the two haptens were as follows:…”

Monoclonal antibody production and development of immunochromatographic strip assays for screening of the herbicide bispyribac-sodium in rice

“…2a shows the components and assembly of the ICSA. 29 The goat anti-mouse antibody and BIS-OVA were sprayed onto the control (C) and test (T) lines of the NC membrane at 0.9 Analytical Methods Paper mL cm −1 . Finally, the assembled base card was cut into 3 mm strips and inserted into the card case as previously reported.…”