A Gold Nanoparticle‐Based Aptamer Target Binding Readout for ATP Assay

Wang, Jing; Wang, Lihua; Liu, Xingfen; Liang, Zhiqiang; Song, Shiping; Li, Wenxin; Li, Genxi; Fan, Chunhai

doi:10.1002/adma.200602256

Cited by 382 publications

(265 citation statements)

References 25 publications

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“…22,23 The generally accepted adsorption model for this process is shown in Figure 1B, where DNA wraps around the AuNP. 22,[24][25][26] The adsorption of non-thiolated DNA has also found important applications in analytical chemistry, [27][28][29][30][31][32][33][34] polymerase chain reactions, 35,36 nanoparticle synthesis, 37,38 and medicine. 37,39 We recently reported that at neutral pH, each 13 nm AuNP adsorbs just around twenty 12-mer DNAs even after overnight incubation, 40 which is consistent with the results from the literature using polyadenosine (e.g A20) as an anchoring block for DNA adsorption with the salt aging approach.…”

Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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The last 16 years have witnessed the landmark development of polyvalent thiolated DNA functionalized gold nanoparticles (AuNPs) possessing striking properties within the emerging field of nanobiotechnology. Many novel properties of this hybrid nanomaterial are attributed to the dense DNA shell. However, the question of whether non-thiolated polyvalent DNA-AuNP could be fabricated with high DNA density and similar properties as its thiolated counterpart has not been explored in detail.Herein, we report that by simply tuning the pH of the DNA/AuNP mixture, an ultrahigh capacity of nonthiolated DNA can be conjugated to AuNPs in a few minutes, resulting in polyvalent DNA-AuNP conjugates with cooperative melting behavior, a typical property for polyvalent thiolated DNA functionalized AuNPs. With this method, large AuNPs (e.g., 50 nm) can be functionalized to achieve colorimetric detection of sub-nM DNA. Further, this fast and stable DNA loading was employed to separate AuNPs of different size. We propose that a large fraction of the attached DNAs are adsorbed via one or a few terminal bases to afford the high loading capacity and the ability to hybridize with the complementary DNA. This discovery not only offers a time-and cost-effective way to functionalize AuNPs with a high density of non-thiolated DNA, but also provides new insights into the fundamental understanding of how DNA strands with different sequences interact with AuNPs.

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Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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“…23,25 For the other approach, ss-DNA is tightly adsorbed onto both nanomaterials, [18][19][20][21] where AuNPs adsorb DNA bases via strong chemical bonding interactions while GO adsorbs DNA via aromatic stacking and hydrophobic interactions. 17 Such adsorption has also been used in biosensor development, [18][19][20][21][26][27][28][29][30][31][32][33] PCR optimization, 34 and materials synthesis. 35,36 A recent research direction is to use these hybrid materials for cellular and in vivo systems.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

et al. 2012

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Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular proteins are present, another layer of complexity is added. In this context, we employ polyethylene glycol (PEG) as a model polymer to mimic the excluded volume effect of cellular proteins and test its effect on DNA adsorption and hybridization on gold nanoparticles (AuNPs) and graphene oxide (GO), both of which show great promise for designing intracellular biosensors and drug delivery systems. We show that PEG 20,000 (e.g. 4%) accelerates DNA hybridization to DNAfunctionalized AuNPs by 50-100%, but this enhanced hybridization kinetics has not been observed with free DNA. Therefore, this rate enhancement is attributed to the surface blocking effect by PEG instead of the macromolecular crowding effect. On the other hand, DNA adsorption on citrate-capped AuNP surfaces is impeded even in the presence of a trace level (i.e., parts-per-billion) of PEG, confirming PEG competes with DNA for surface binding sites. Additional insights have been obtained by studying the adsorption of a thiolated DNA and a peptide nucleic acid. In these cases, the steric effects of PEG to impede adsorption are observed. Similar observations have also been made with GO. Therefore, PEG may be used as an effective blocking agent for both hydrophilic AuNP and for GO that also contains hydrophobic domains.

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“…Therefore, AuNPs have been used as a probe for DNA secondary structure and related analytical applications. [25][26][27][28][29][30][31][32] DNA adsorption has also been used to control the growth of AuNPs, 33,34 tune catalytic activities of AuNPs, 35,36 and improve the specificity of polymerase chain reactions (PCR). 37 47 Finally, applications related to drug delivery can also be envisioned.…”

Section: Introductionmentioning

confidence: 99%

Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles

2012

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Single-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental interaction of this adsorption reaction remains unclear. In this study, we measured DNA adsorption kinetics, capacity, and isotherms, demonstrating that the adsorption process is governed by electrostatic forces. The charge repulsion among DNA strands and between DNA and AuNPs can be reduced by adding salt, reducing pH or by using non-charged peptide nucleic acid (PNA).Langmuir adsorption isotherms are obtained, indicating the presence of both adsorption and desorption of DNA from AuNPs. While increasing salt concentration facilitates DNA adsorption, the desorption rate is also enhanced in higher salt due to DNA compaction. DNA adsorption capacity is determined by both DNA oligomer length, DNA concentration and salt. Previous studies indicated faster adsorption of short DNA oligomers by AuNPs, we find that once adsorbed, longer DNAs are much more effective in protecting AuNPs from aggregation. DNA adsorption is also facilitated by using low pH buffers and high alcohol concentrations. A model based on electrostatic repulsion on

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A Gold Nanoparticle‐Based Aptamer Target Binding Readout for ATP Assay

Cited by 382 publications

References 25 publications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles

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