A gold nanoparticle strip for simultaneously evaluating FMDV immunized antibody level and discriminating FMDV vaccinated animals from infected animals

Abstract

A gold nanoparticle strip was developed for rapidly evaluating FMDV type O antibody level and simultaneously discriminating FMDV vaccinated animals from infected animals.

Lateral flow immunoassays for antigens, antibodies and haptens detection

“…In particular, low sensitivity to trace specific antibody often cause false negative results. A gold nanoparticle strip discriminating Foot-and-Mouth disease virus (FMDV) vaccinated animals from infected animals was established [ 25 ]. SPA conjugated with colloidal gold nanoparticles were used as a probe.…”

Application of Noble Metals in the Advances in Animal Disease Diagnostics

“…Foot-and-Mouth Disease (FMD) is one of the most devastating and highly contagious disease of cloven-hoofed animals (e.g. ruminants and swine) that may threaten food security [34]. The causative agent, Foot-and-Mouth Disease virus (FMDV) has multiple modes of transmission, fast replication rate and viral excretion that makes FMD one of the acute and highly contagious diseases of cloven-hoofed animals [34,35].…”

“…ruminants and swine) that may threaten food security [34]. The causative agent, Foot-and-Mouth Disease virus (FMDV) has multiple modes of transmission, fast replication rate and viral excretion that makes FMD one of the acute and highly contagious diseases of cloven-hoofed animals [34,35]. Southeast and East Asian countries such as Cambodia, Laos, Thailand, Vietnam and China show varying FMD prevalence [34].…”

“…Conventionally, serological methods such as FMD structural proteins (SPs)-based virus neutralization test (VNT), liquid phase blocking enzyme-linked immunosorbent assay (LPB-ELISA) and solid-phase competition ELISA (SPCE) can evaluate the antibody level and non-structural proteins (NSPs)-based ELISA can discriminate naturally infected from immunized animals. However, with the advent and success of immunochromatographic strip (ICS) in the field due to its high specificity, sensitivity, rapidity, low cost and portability for field detection and high sample throughput, Yang et al [34] developed an immunochromatographic gold nanoparticle strip that can differentiate FMD type O-naturally infected from immunized animals using serum. Both epitopes of FMDVs SPs (T1) and NSPs (T2) were dispensed in the nitrocellulose membranes to be the two test lines and as for control line a goat anti-pig antibody IgG was used.…”