A Graphene Oxide-Based Fluorescent Aptasensor for the Turn-on Detection of CCRF-CEM

Tan, Jie; Lai, Zongqiang; Zhong, Liping; Zhang, Zhenghua; Zheng, Rong; Su, Jing; Huang, Yong; Huang, Panpan; Song, Hui; Yang, Nuo; Zhou, Sufang; Zhao, Yongxiang

doi:10.1186/s11671-017-2403-3

Cited by 24 publications

(16 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tan et al 51 have shown that the fluorescence quenching of FAM-aptamer occurs rapidly following its binding to the GO surface (i.e. the formation of FAM-aptamer-GO composite) and tends to decrease significantly after 2 min.…”

Section: Discussionmentioning

confidence: 99%

DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

Pourhajibagher

Etemad‐Moghadam

Alaeddini

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The aim of this study was to design and evaluate the specificity of a targeted bio-theragnostic system based on DNA-aptamer-nanographene oxide (NGO) against Porphyromonas gingivalis during antimicrobial photodynamic therapy (aPDT). Following synthesis and confirmation of NGO, the binding of selected labeled DNA-aptamer to NGO was performed and its hemolytic activity, cytotoxic effect, and release times were evaluated. The specificity of DNA-aptamer-NGO to P. gingivalis was determined. The antimicrobial effect, anti-biofilm potency, and anti-metabolic activity of aPDT were then assessed after the determination of the bacteriostatic and bactericidal concentrations of DNA-aptamer-NGO against P. gingivalis. Eventually, the apoptotic effect and anti-virulence capacity of aPDT based on DNA-aptamer-NGO were investigated. The results showed that NGO with a flaky, scale-like, and layered structure in non-cytotoxic DNA-aptamer-NGO has a continuous release in the weak-acid environment within a period of 240 h. The binding specificity of DNA-aptamer-NGO to P. gingivalis was confirmed by flow cytometry. When irradiated, non-hemolytic DNA-aptamer-NGO were photoactivated, generated ROS, and led to a significant decrease in the cell viability of P. gingivalis (P < 0.05). Also, the data indicated that DNA-aptamer-NGO-mediated aPDT led to a remarkable reduction of biofilms and metabolic activity of P. gingivalis compared to the control group (P < 0.05). In addition, the number of apoptotic cells increased slightly (P > 0.05) and the expression level of genes involved in bacterial biofilm formation and response to oxidative stress changed significantly after exposure to aPDT. It is concluded that aPDT using DNA-aptamer-NGO as a targeted bio-theragnostic system is a promising approach to detect and eliminate P. gingivalis as one of the main bacteria involved in periodontitis in periopathogenic complex in real-time and in situ.

show abstract

Section: Discussionmentioning

confidence: 99%

DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

Pourhajibagher

Etemad‐Moghadam

Alaeddini

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Recently, graphene oxide (GO) has gained a great deal of interest for virus detection due to its large surface area, 2D carbon structure, electrical conductivity, thermal robustness, flexibility, optical transparency, and mechanical characteristics, with high chemical stability [ 29 , 30 , 31 , 32 , 33 , 34 ]. Aptamers have demonstrated that graphene possesses unique attachment, molecular recognition, and biocompatibility via linking them with graphene to enhance the sensitivity and selectivity of the manufactured biosensors.…”

Section: Graphene Oxide Fluorescent Biosensor For Human Virus Detectionmentioning

confidence: 99%

Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials

2022

View full text Add to dashboard Cite

Two-dimensional carbon nanomaterials have been commonly employed in the field of biosensors to improve their sensitivity/limits of detection and shorten the analysis time. These nanomaterials act as efficient transducers because of their unique characteristics, such as high surface area and optical, electrical, and magnetic properties, which in turn have been exploited to create simple, quick, and low-cost biosensing platforms. In this review, graphene and two-dimensional carbon material-based fluorescent biosensors are covered between 2010 and 2021, for the detection of different human viruses. This review specifically focuses on the new developments in graphene and two-dimensional carbon nanomaterials for fluorescent biosensing based on the Förster resonance energy transfer (FRET) mechanism. The high-efficiency quenching capability of graphene via the FRET mechanism enhances the fluorescent-based biosensors. The review provides a comprehensive reference for the different types of carbon nanomaterials employed for the detection of viruses such as Rotavirus, Ebola virus, Influenza virus H3N2, HIV, Hepatitis C virus (HCV), and Hepatitis B virus (HBV). This review covers the various multiplexing detection technologies as a new direction in the development of biosensing platforms for virus detection. At the end of the review, the different challenges in the use of fluorescent biosensors, as well as some insights into how to overcome them, are highlighted.

show abstract

“…The LOD correlates with those of many previous reports on sgc8 aptamermediated CCRF-CEM cell detection, indicating that our design maintained the selectivity and sensitivity of the aptamer as the key catching agent. 56,57 In summary, we have developed a miniature iCatch device for cell sorting integrated with thermo-responsive aptamer catch-release and PNIPAAm hydrogel swelling and contraction to realize the target cell spatial movement in a synergistic way. It is observed that the iCatch device shows high throughput (888 cells mm À1 s À1 ), efficiency (39.4% in 15 s) and effectiveness (470% recovery rate) on CCRF-CEM cell sorting.…”

Section: Evaluation Of Icatch Capability Of Selective Cell Catchtrans...mentioning

confidence: 99%

A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Zhang

Wang

et al. 2021

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

A Graphene Oxide-Based Fluorescent Aptasensor for the Turn-on Detection of CCRF-CEM

Cited by 24 publications

References 39 publications

DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials

A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Contact Info

Product

Resources

About